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The map label for this gene is purB [H]

Identifier: 21909566

GI number: 21909566

Start: 52051

End: 53343

Strand: Direct

Name: purB [H]

Synonym: SpyM3_0030

Alternate gene names: 21909566

Gene position: 52051-53343 (Clockwise)

Preceding gene: 21909565

Following gene: 21909567

Centisome position: 2.74

GC content: 44.86

Gene sequence:

>1293_bases
ATGATTGAACGTTATTCACGCCCTGAGATGGCGGCAATTTGGACAGAGGAAAATAAATACCGTGCTTGGTTGGAGGTTGA
GATTCTGGCTGACGAGGCATGGGCAGAGTTGGGTGAGATTCCTAAGGAGGATGTGGCTAAGATTCGTGAGAAGGCGGATT
TTGACATTGACCGCATTCTTGAGATTGAGCAGGAGACGCGTCACGATGTGGTGGCTTTCACGCGTGCAGTTTCTGAGACG
CTTGGTGAGGAGCGCAAGTGGGTGCACTACGGTTTGACCTCGACTGACGTGGTGGACACTGCCTACGGTTACCTCTACAA
GCAAGCTAACGACATTATCCGTCGCGATCTTGAGAATTTCACCAATATCGTGGCAGACAAGGCGCGTGAGCACAAAATGA
CCATCATGATGGGTCGTACCCACGGTGTTCATGCCGAGCCAACGACTTTCGGTCTTAAGTTGGCGACTTGGTACAGCGAG
ATGAAACGTAATCAGGAGCGTTTTGAACATGCTGCCGCAGGTGTGGAAGCTGGTAAGATTTCAGGTGCCGTTGGTAACTT
TGCTAATATCCCTCTATTTGTGGAACAATATGTCTGTGACAAATTAGGCATTCGTTCACAAGAGATTTCAACACAAGTTC
TTCCACGTGACCTCCACGCAGAATATTTTGCAGTGCTTGCAAGTATTGCAACTTCTATCGAACGTATGGCGACAGAGATT
CGTGGTCTGCAAAAGTCAGAACAACGTGAAGTTGAAGAATTCTTTGCCAAAGGTCAGAAAGGTAGCTCTGCTATGCCTCA
CAAACGCAACCCAATCGGTTCAGAGAACATGACAGGGCTAGCGCGCGTGATTCGTGGTCACATGGTGACAGCTTATGAGA
ACGTGTCACTTTGGCATGAGCGTGATATTTCACACTCATCAGCTGAGCGTATCATCACACCTGACACAACTATCTTGATT
GACTACATGCTCAACCGCTTTGGCAATATCGTTAAGAACTTGACTGTCTTCCCGGAAAATATGATGCGCAATATGGAATC
AACTTTTGGTTTGATTTATAGTCAACGTGTTATGCTCAAATTGATTGAAAAAGGAATGACACGAGAAGAAGCTTATGACT
TAGTTCAACCTAAGACAGCTTATTCCTGGGACAATCAAGTGGATTTCAAACCACTTTTAGAAGAAGACACCAAAGTTACC
TCTTGTCTTACACAAGAAGAAATTGATGAACTATTTAATCCGATTTATTACACAAAACGTGTTGATGATATTTTTAAGCG
TTTAGGGATTTAA

Upstream 100 bases:

>100_bases
AGCGGAAAGCTCGGAATTTAGATAAACCGCCTAGGACGCAAGCGTCCATTGTTGGTTTCCTAAAATTCAGTCGCTTTCTG
GTCGCCCTTGGTATCTTAAA

Downstream 100 bases:

>100_bases
TATAAAAAAAAGAAGGTGATTCCCTTCTTTTTTATTTAAAAAACGAATGTTTTCTTTAAAAATATTAAAAATGTTAGTTT
TTAATTAGACGCTGTGATAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKEDVAKIREKADFDIDRILEIEQETRHDVVAFTRAVSET
LGEERKWVHYGLTSTDVVDTAYGYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
MKRNQERFEHAAAGVEAGKISGAVGNFANIPLFVEQYVCDKLGIRSQEISTQVLPRDLHAEYFAVLASIATSIERMATEI
RGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILI
DYMLNRFGNIVKNLTVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPLLEEDTKVT
SCLTQEEIDELFNPIYYTKRVDDIFKRLGI

Sequences:

>Translated_430_residues
MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKEDVAKIREKADFDIDRILEIEQETRHDVVAFTRAVSET
LGEERKWVHYGLTSTDVVDTAYGYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
MKRNQERFEHAAAGVEAGKISGAVGNFANIPLFVEQYVCDKLGIRSQEISTQVLPRDLHAEYFAVLASIATSIERMATEI
RGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILI
DYMLNRFGNIVKNLTVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPLLEEDTKVT
SCLTQEEIDELFNPIYYTKRVDDIFKRLGI
>Mature_430_residues
MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKEDVAKIREKADFDIDRILEIEQETRHDVVAFTRAVSET
LGEERKWVHYGLTSTDVVDTAYGYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
MKRNQERFEHAAAGVEAGKISGAVGNFANIPLFVEQYVCDKLGIRSQEISTQVLPRDLHAEYFAVLASIATSIERMATEI
RGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILI
DYMLNRFGNIVKNLTVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPLLEEDTKVT
SCLTQEEIDELFNPIYYTKRVDDIFKRLGI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=446, Percent_Identity=26.0089686098655, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI183227688, Length=398, Percent_Identity=27.3869346733668, Blast_Score=155, Evalue=9e-38,
Organism=Escherichia coli, GI1787376, Length=303, Percent_Identity=29.042904290429, Blast_Score=99, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17508577, Length=396, Percent_Identity=26.010101010101, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564234, Length=351, Percent_Identity=25.6410256410256, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323391, Length=430, Percent_Identity=27.6744186046512, Blast_Score=156, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24647570, Length=391, Percent_Identity=24.2966751918159, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49588; Mature: 49588

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKEDVAKIREKADFDIDRIL
CCCCCCCCCEEEEEECCCCEEEEEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCCHHHHH
EIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAYGYLYKQANDIIRRDLENF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSEMKRNQERFEHAAAGVEAGKI
HHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
SGAVGNFANIPLFVEQYVCDKLGIRSQEISTQVLPRDLHAEYFAVLASIATSIERMATEI
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMVTAYENVSLWHE
HHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCHHHHH
RDISHSSAERIITPDTTILIDYMLNRFGNIVKNLTVFPENMMRNMESTFGLIYSQRVMLK
HCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LIEKGMTREEAYDLVQPKTAYSWDNQVDFKPLLEEDTKVTSCLTQEEIDELFNPIYYTKR
HHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
VDDIFKRLGI
HHHHHHHHCC
>Mature Secondary Structure
MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKEDVAKIREKADFDIDRIL
CCCCCCCCCEEEEEECCCCEEEEEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCCHHHHH
EIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAYGYLYKQANDIIRRDLENF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSEMKRNQERFEHAAAGVEAGKI
HHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
SGAVGNFANIPLFVEQYVCDKLGIRSQEISTQVLPRDLHAEYFAVLASIATSIERMATEI
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMVTAYENVSLWHE
HHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCHHHHH
RDISHSSAERIITPDTTILIDYMLNRFGNIVKNLTVFPENMMRNMESTFGLIYSQRVMLK
HCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LIEKGMTREEAYDLVQPKTAYSWDNQVDFKPLLEEDTKVTSCLTQEEIDELFNPIYYTKR
HHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
VDDIFKRLGI
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]