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The map label for this gene is purN [H]

Identifier: 21909559

GI number: 21909559

Start: 43504

End: 44058

Strand: Direct

Name: purN [H]

Synonym: SpyM3_0023

Alternate gene names: 21909559

Gene position: 43504-44058 (Clockwise)

Preceding gene: 21909558

Following gene: 21909560

Centisome position: 2.29

GC content: 47.03

Gene sequence:

>555_bases
ATGAAAATCGCTGTTTTTGCTTCTGGTAATGGTTCCAACTTTCAGGTCATAGCAGAGCAGTTTCCAGTTAGTTTTGTCTT
TTCAGATCATCGTGATGCCTATGTTTTAGAGCGTGCTCAGAATTTAGCCATTCCAAGCTTCGCTTTTGAACTCAAGGAGT
TTGAGAATAAGGCGGCTTACGAGCAAGCCATCGTTGATTTGTTGGACAAACACGAGATTGATTTGGTCTGTCTGGCGGGC
TATATGAAGATTGTTGGAGAAACCTTACTCTTGGCCTATGAGGGGCGTATTATCAATATTCACCCAGCCTACCTGCCTGA
ATTTCCAGGTGCCCACGGTATCGAGGATGCTTGGGAAGCTGGTGTTGACCAGTCTGGCGTGACCATCCACTGGGTTGACT
CTGGTGTGGATACCGGTCAGGTCATCCAACAAGTGCGCGTGCCACGCCTAGCAGACGATAGCCTAGAAAGCTTTGAAACC
CGTATCCATGAAACCGAATATCAACTCTACCCAGCTGTCTTAGATAGCTTGGGAGTGGAGAGGAAAGTAATCTAG

Upstream 100 bases:

>100_bases
AGGAAAAAAGATAAAACGACTTAGACGCAAGCGTCGTTGTTCGTTTTTCTATTTTTCTACGAAATTTTTCGGCAAGCCGA
ATCATCCCTTTGTATCGAAT

Downstream 100 bases:

>100_bases
CCTTTATCTTAAAATTTTAGGTAAAGTTAGGAAAGATAGTAAAGAAGAATTAACGTTCCAAAAAGAAAGGAATTGAACCC
GTCTGGAAGGCACAGAAAAA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAIPSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLAG
YMKIVGETLLLAYEGRIINIHPAYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
RIHETEYQLYPAVLDSLGVERKVI

Sequences:

>Translated_184_residues
MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAIPSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLAG
YMKIVGETLLLAYEGRIINIHPAYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
RIHETEYQLYPAVLDSLGVERKVI
>Mature_184_residues
MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAIPSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLAG
YMKIVGETLLLAYEGRIINIHPAYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
RIHETEYQLYPAVLDSLGVERKVI

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=180, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=4e-28,
Organism=Homo sapiens, GI209869995, Length=180, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=4e-28,
Organism=Homo sapiens, GI209869993, Length=180, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=4e-28,
Organism=Escherichia coli, GI1788846, Length=181, Percent_Identity=36.4640883977901, Blast_Score=128, Evalue=2e-31,
Organism=Escherichia coli, GI1787483, Length=98, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=31.4917127071823, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=29.7979797979798, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582400, Length=185, Percent_Identity=37.2972972972973, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20554; Mature: 20554

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAIPSFAFELKEFENKAAY
CEEEEEECCCCCCEEEEHHHCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EQAIVDLLDKHEIDLVCLAGYMKIVGETLLLAYEGRIINIHPAYLPEFPGAHGIEDAWEA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEECCEEEEECCCCCCCCCCCCCCCHHHHC
GVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFETRIHETEYQLYPAVLDSLGVE
CCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RKVI
CCCC
>Mature Secondary Structure
MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAIPSFAFELKEFENKAAY
CEEEEEECCCCCCEEEEHHHCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EQAIVDLLDKHEIDLVCLAGYMKIVGETLLLAYEGRIINIHPAYLPEFPGAHGIEDAWEA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEECCEEEEECCCCCCCCCCCCCCCHHHHC
GVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFETRIHETEYQLYPAVLDSLGVE
CCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RKVI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]