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The map label for this gene is mesJ [H]

Identifier: 21909546

GI number: 21909546

Start: 11049

End: 12236

Strand: Direct

Name: mesJ [H]

Synonym: SpyM3_0010

Alternate gene names: 21909546

Gene position: 11049-12236 (Clockwise)

Preceding gene: 21909545

Following gene: 21909547

Centisome position: 0.58

GC content: 28.62

Gene sequence:

>1188_bases
ATGAATTTATTGCACTTTTTATACCTGTTTCAAGACAAATTGAAAATTCGTATTGGTATTGCTCATGTTAATCACAAACA
AAGATCAGAATCAGATTCAGAAGAAGCTTATTTAAAGTGTTGGGCTAAAAAACATGACATACCTATTTATGTTTCTAATT
TTGAAGGAATATTTTCAGAAAAAGCAGCACGTGATTGGCGCTATGCTTTTTTTAAGAGTATTATGTTAAAAAATAACTAT
TCAGCTTTAGTAACAGCTCACCATTCAGATGATCAAGCAGAAACTATTTTGATGAGGCTAATTAGAGGAAGCCGATTGAG
GCACTTGTCGGGTATAAAAAGCGTGCAACCATTTGCCAATGGTCAACTTATAAGACCTTTTTTAACTTTCTCAAAAAAGG
ACTTGCCAGAAATTTTTCATTTTGAAGATTCTTCAAATAGGGAATTATCATTTTTAAGAAATAGAGTTAGAAATAATTAT
TTACCATTGTTAAAGCAGGAAAATCCTAGGTTCATTCAGGGATTAAATCAATTAGCACTTGAAAATAGCTTACTTTTTCA
AGCTTTTAAGGAGCTAACAAATCATATTACTACCACTGATTTAACAGAATTTAATGAGCAATCTAAGTCTATTCAATACT
TTTTGTTACAAGATTATTTAGAAGGTTTTCCAGATTTAGATTTAAAAAAATCTCAATTTACTCAATTATTGCAAATTATC
CAAACTGCTAAACAAGGCTATTATTATCTCAAAAAAGACTATTATATATTCATTGATAAATTCTCTTTTAAAATTACAAA
AATCGTTCCTAAGACTGAGTTAGTTAAAGATGAAAAAATGTTAGAATACGATAGTAACTTATGCTACAGAGATTATTGTT
TTTCATTTATGCCAAAAAGTAACGAAGATCAAGGACAGGTAAGTATACCTTTATTTAGTTTATCGTCTATTAAATTAAGA
AGTCGCCAATCTGGAGATTATATTTCTTTTGGTCACTTTTCAAAAAAAATAAGACGTTTATTTATAGATGAAAAATTCAC
AATAGCTGAACGCCAAAATGCTATTATTGGAGAGCAAGATGAGCAGATTATATTTGTATTGATAGGTAATAAAACTTATT
TGAGAAAAGCTTGTAAACATGATATAATGTTGGCTAAACTGTATATTGATAAATTAGAAAAAGGGTGA

Upstream 100 bases:

>100_bases
AATGATGACCTATCAAGAAATTTTTAATGAAATAAAAAATAAAGCCTACTTTAAAAATCATAGACATGTTTTAATAGCCG
TATCTGGTGGAGTAGATTCA

Downstream 100 bases:

>100_bases
CAGCATGCTCGAGCAAGATATTCAAAAAATTCTTTACTCTGAAAATGATATTATTCGGAAAACCAAAAAATTAGGAGAAC
AATTAACAAAAGATTATCAA

Product: putative cell-cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNLLHFLYLFQDKLKIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSEKAARDWRYAFFKSIMLKNNY
SALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFANGQLIRPFLTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNY
LPLLKQENPRFIQGLNQLALENSLLFQAFKELTNHITTTDLTEFNEQSKSIQYFLLQDYLEGFPDLDLKKSQFTQLLQII
QTAKQGYYYLKKDYYIFIDKFSFKITKIVPKTELVKDEKMLEYDSNLCYRDYCFSFMPKSNEDQGQVSIPLFSLSSIKLR
SRQSGDYISFGHFSKKIRRLFIDEKFTIAERQNAIIGEQDEQIIFVLIGNKTYLRKACKHDIMLAKLYIDKLEKG

Sequences:

>Translated_395_residues
MNLLHFLYLFQDKLKIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSEKAARDWRYAFFKSIMLKNNY
SALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFANGQLIRPFLTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNY
LPLLKQENPRFIQGLNQLALENSLLFQAFKELTNHITTTDLTEFNEQSKSIQYFLLQDYLEGFPDLDLKKSQFTQLLQII
QTAKQGYYYLKKDYYIFIDKFSFKITKIVPKTELVKDEKMLEYDSNLCYRDYCFSFMPKSNEDQGQVSIPLFSLSSIKLR
SRQSGDYISFGHFSKKIRRLFIDEKFTIAERQNAIIGEQDEQIIFVLIGNKTYLRKACKHDIMLAKLYIDKLEKG
>Mature_395_residues
MNLLHFLYLFQDKLKIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSEKAARDWRYAFFKSIMLKNNY
SALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFANGQLIRPFLTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNY
LPLLKQENPRFIQGLNQLALENSLLFQAFKELTNHITTTDLTEFNEQSKSIQYFLLQDYLEGFPDLDLKKSQFTQLLQII
QTAKQGYYYLKKDYYIFIDKFSFKITKIVPKTELVKDEKMLEYDSNLCYRDYCFSFMPKSNEDQGQVSIPLFSLSSIKLR
SRQSGDYISFGHFSKKIRRLFIDEKFTIAERQNAIIGEQDEQIIFVLIGNKTYLRKACKHDIMLAKLYIDKLEKG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=182, Percent_Identity=32.4175824175824, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 46649; Mature: 46649

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLHFLYLFQDKLKIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSE
CCHHHHHHHHHCCEEEEEEEEECCCHHCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHH
KAARDWRYAFFKSIMLKNNYSALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFAN
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCC
GQLIRPFLTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNYLPLLKQENPRFIQGLNQLAL
CCEEHHHHHCCCCCCCHHEECCCCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHH
ENSLLFQAFKELTNHITTTDLTEFNEQSKSIQYFLLQDYLEGFPDLDLKKSQFTQLLQII
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
QTAKQGYYYLKKDYYIFIDKFSFKITKIVPKTELVKDEKMLEYDSNLCYRDYCFSFMPKS
HHHHCCEEEEECCEEEEEEECCEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
NEDQGQVSIPLFSLSSIKLRSRQSGDYISFGHFSKKIRRLFIDEKFTIAERQNAIIGEQD
CCCCCEEEEEEEECCCEEEECCCCCCEEEHHHHHHHHHHHHCCCCEEHHHHCCCCCCCCC
EQIIFVLIGNKTYLRKACKHDIMLAKLYIDKLEKG
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLLHFLYLFQDKLKIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSE
CCHHHHHHHHHCCEEEEEEEEECCCHHCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHH
KAARDWRYAFFKSIMLKNNYSALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFAN
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCC
GQLIRPFLTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNYLPLLKQENPRFIQGLNQLAL
CCEEHHHHHCCCCCCCHHEECCCCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHH
ENSLLFQAFKELTNHITTTDLTEFNEQSKSIQYFLLQDYLEGFPDLDLKKSQFTQLLQII
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
QTAKQGYYYLKKDYYIFIDKFSFKITKIVPKTELVKDEKMLEYDSNLCYRDYCFSFMPKS
HHHHCCEEEEECCEEEEEEECCEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
NEDQGQVSIPLFSLSSIKLRSRQSGDYISFGHFSKKIRRLFIDEKFTIAERQNAIIGEQD
CCCCCEEEEEEEECCCEEEECCCCCCEEEHHHHHHHHHHHHCCCCEEHHHHCCCCCCCCC
EQIIFVLIGNKTYLRKACKHDIMLAKLYIDKLEKG
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]