Click here to switch to the map view.

The map label for this gene is murI

Identifier: 21672799

GI number: 21672799

Start: 590218

End: 590988

Strand: Direct

Name: murI

Synonym: BUsg536

Alternate gene names: 21672799

Gene position: 590218-590988 (Clockwise)

Preceding gene: 21672798

Following gene: 21672801

Centisome position: 92.01

GC content: 20.75

Gene sequence:

>771_bases
GTGCTTATAATCGATTCTGGTATAGGTGGGCTATCTATATTAGATAATATTAAAAAAAAATTTCCCCATATAAATTATAT
TTATATGCTAGATAACGAAGCTTTTCCTTATGGAAAAAAAAAAGAAAAATTTCTTATAGAAAGAAGCATAAAAATAATTA
ATGCAATAAAGAAAATTTATCCTATTAAGATGGTAATAATAGCTTGTAATACAGCAAGTACAATATCTTTACCTACTTTA
AAAAAAACTTTTAGTATTCCTATTATTGGAGTGTTACCTGTATTTAAACCTGCTATTAAAATAACAAAAAATAAAATTAT
CGGTTTAATAGCAACTCGATCAACTATTAATAGTTTATATATAAAAAAAACAATATATAAATATTCTCTTGAAAATACTA
TAAAAATAATAGCTACAAATGAACTAGCGGTAATAGCTGAAAAAAAAGTTAGAAAACTATCTATTTCAAATATAAAATTA
AAAAAAATCTTTCAATCGTGGATCATTCTTTCAATAAAACCTGATACTATCATCCTTGGATGTACTCATTTTTCTTTTCT
AAAAAAAGAGATTCAGCAGATATTTCATAAACCTATTAATTTTATAGACCCAGGAGATACCATTGTTAATAAAATTGAAA
AATATTTTTATCAAAAAAAAATAAAAAAAAATATTTTATTATGTTCGAAATATAATAAACAAATAAAACAACTAGTATTT
TTTTTAAAAAAATATAAATTTAAAAAAATTCAAGAAATTAACTTAAATTAA

Upstream 100 bases:

>100_bases
ATATATGAAATTATTTCTATTTAATAACGAATAGACACATTTTTCTATATAAATTTAAATTATTAATATAATTTCTTAAA
ATTATTAGCGTATTAAAAAA

Downstream 100 bases:

>100_bases
TATTTTTATAAAACACTTTTTTATATTTTTTAAAAACATACTTTAATAAATCAGATAATAAATTATTTTTTTTTACATCA
TAAGAATAAATTTTTAAAAG

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLIIDSGIGGLSILDNIKKKFPHINYIYMLDNEAFPYGKKKEKFLIERSIKIINAIKKIYPIKMVIIACNTASTISLPTL
KKTFSIPIIGVLPVFKPAIKITKNKIIGLIATRSTINSLYIKKTIYKYSLENTIKIIATNELAVIAEKKVRKLSISNIKL
KKIFQSWIILSIKPDTIILGCTHFSFLKKEIQQIFHKPINFIDPGDTIVNKIEKYFYQKKIKKNILLCSKYNKQIKQLVF
FLKKYKFKKIQEINLN

Sequences:

>Translated_256_residues
MLIIDSGIGGLSILDNIKKKFPHINYIYMLDNEAFPYGKKKEKFLIERSIKIINAIKKIYPIKMVIIACNTASTISLPTL
KKTFSIPIIGVLPVFKPAIKITKNKIIGLIATRSTINSLYIKKTIYKYSLENTIKIIATNELAVIAEKKVRKLSISNIKL
KKIFQSWIILSIKPDTIILGCTHFSFLKKEIQQIFHKPINFIDPGDTIVNKIEKYFYQKKIKKNILLCSKYNKQIKQLVF
FLKKYKFKKIQEINLN
>Mature_256_residues
MLIIDSGIGGLSILDNIKKKFPHINYIYMLDNEAFPYGKKKEKFLIERSIKIINAIKKIYPIKMVIIACNTASTISLPTL
KKTFSIPIIGVLPVFKPAIKITKNKIIGLIATRSTINSLYIKKTIYKYSLENTIKIIATNELAVIAEKKVRKLSISNIKL
KKIFQSWIILSIKPDTIILGCTHFSFLKKEIQQIFHKPINFIDPGDTIVNKIEKYFYQKKIKKNILLCSKYNKQIKQLVF
FLKKYKFKKIQEINLN

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=258, Percent_Identity=36.8217054263566, Blast_Score=216, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_BUCAP (Q8K924)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660866.1
- ProteinModelPortal:   Q8K924
- SMR:   Q8K924
- EnsemblBacteria:   EBBUCT00000000251
- GeneID:   1005570
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg536
- GeneTree:   EBGT00050000007930
- HOGENOM:   HBG645102
- OMA:   CGHIPYG
- ProtClustDB:   CLSK316489
- BioCyc:   BAPH198804:BUSG536-MONOMER
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 29628; Mature: 29628

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIDSGIGGLSILDNIKKKFPHINYIYMLDNEAFPYGKKKEKFLIERSIKIINAIKKIY
CEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
PIKMVIIACNTASTISLPTLKKTFSIPIIGVLPVFKPAIKITKNKIIGLIATRSTINSLY
CEEEEEEEECCCCEEECCCHHHHHCCCEEEHHHHHHHHHHHHHCCEEEEEEEHHHHHHHH
IKKTIYKYSLENTIKIIATNELAVIAEKKVRKLSISNIKLKKIFQSWIILSIKPDTIILG
HHHHHHHEECCCEEEEEEECCEEEEEHHHHHHHHCCCHHHHHHHHHHEEEEECCCEEEEE
CTHFSFLKKEIQQIFHKPINFIDPGDTIVNKIEKYFYQKKIKKNILLCSKYNKQIKQLVF
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHH
FLKKYKFKKIQEINLN
HHHHHHHHHHEECCCC
>Mature Secondary Structure
MLIIDSGIGGLSILDNIKKKFPHINYIYMLDNEAFPYGKKKEKFLIERSIKIINAIKKIY
CEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
PIKMVIIACNTASTISLPTLKKTFSIPIIGVLPVFKPAIKITKNKIIGLIATRSTINSLY
CEEEEEEEECCCCEEECCCHHHHHCCCEEEHHHHHHHHHHHHHCCEEEEEEEHHHHHHHH
IKKTIYKYSLENTIKIIATNELAVIAEKKVRKLSISNIKLKKIFQSWIILSIKPDTIILG
HHHHHHHEECCCEEEEEEECCEEEEEHHHHHHHHCCCHHHHHHHHHHEEEEECCCEEEEE
CTHFSFLKKEIQQIFHKPINFIDPGDTIVNKIEKYFYQKKIKKNILLCSKYNKQIKQLVF
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHH
FLKKYKFKKIQEINLN
HHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438