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The map label for this gene is gshB

Identifier: 21672792

GI number: 21672792

Start: 584745

End: 585707

Strand: Direct

Name: gshB

Synonym: BUsg529

Alternate gene names: 21672792

Gene position: 584745-585707 (Clockwise)

Preceding gene: 21672791

Following gene: 30410827

Centisome position: 91.16

GC content: 26.38

Gene sequence:

>963_bases
ATGAGAAAAAAAAAGAATTTAAAAATCGGAATAGTAATGGATTCTATTACATTAATTAACATCAAAAAAGACTCAAGTTT
TGCTATATTATTAGAAGCTCAAAAAAGACAACATGAAATTTATTATATGGAAATGAATGATCTCTATTTAAGGAAAGGTC
AATCGTATGCAACAACAAAATCAATAGAAATTCAAAAAAATCAAAATAATTATTTTAAATTTATTCAAAAAAAAGATATT
TCATTAAATGAGTTAGATGCTATTTTAATGCGAAAAGATCCTCCATTTAATACTGAATTTATTTACGCAACATATATTCT
CGAACGTGCAGAAGAAAAAGGTGTATTAGTAATTAACAAACCCAAAAGTTTAAGAGACTGCAACGAAAAAATATTTATAT
CATGGTTTTCTAGATTTACTACAGACACGTTAGTTACAAGAAAATTATCTAAAATACATAATTTTTGGAAAGAAAAAAAC
GACATAATATTAAAACCCTTAGATGCAATGGGTGGGAAAGGTGTCTTTCGTATTAAGAAAGATGACCCTAATTTTTCTGT
TATTGTAGAAACACTTACAAATTACGAAAAGAAATATTGTATGATTCAAACTTATTTACCAGAAGTACAATTCGGTGACA
AAAGAATTTTAATTGTAAATGGAAAACCTATACCTTGGTCTTTAACACGTATTCCTAAACATGGAGAAACAAGAGCAAAT
TTGGCTGTTGGAGGAGAAGGAAGGGTACAGAAACTAAACGATAAAGATTGGGAAATAGCTAATTATTTGGCTCCAATTTT
AAAAAAAAGAGGATTAATTCTTGTTGGATTAGATGTAATAGGTGATAAATTAACAGAAATAAATGTTACAAGTCCAACAT
GTATTTGTGAAATTGAAGAAAAGAAAAATATTTCTATTACAGGTATTTTAATCGATTATATTGAAGATAAAATATATAAA
TAG

Upstream 100 bases:

>100_bases
TTAACAACTAATTTTAATTAAGAAAATTGTAATTTTAAAAAAATGCGTTTTGTATTATATTTACAACACATAATTTAATT
TAGTATTGAGTTATAAACAT

Downstream 100 bases:

>100_bases
AGATCAAAAAATGATAGTAATAGCTTTTGATTTTGGTTTAAAGAATATTGGAGTAGCAGTCGGAGAAAATATCCTTAAAA
AAGGCAGAGCTTTAAATAAA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRKKKNLKIGIVMDSITLINIKKDSSFAILLEAQKRQHEIYYMEMNDLYLRKGQSYATTKSIEIQKNQNNYFKFIQKKDI
SLNELDAILMRKDPPFNTEFIYATYILERAEEKGVLVINKPKSLRDCNEKIFISWFSRFTTDTLVTRKLSKIHNFWKEKN
DIILKPLDAMGGKGVFRIKKDDPNFSVIVETLTNYEKKYCMIQTYLPEVQFGDKRILIVNGKPIPWSLTRIPKHGETRAN
LAVGGEGRVQKLNDKDWEIANYLAPILKKRGLILVGLDVIGDKLTEINVTSPTCICEIEEKKNISITGILIDYIEDKIYK

Sequences:

>Translated_320_residues
MRKKKNLKIGIVMDSITLINIKKDSSFAILLEAQKRQHEIYYMEMNDLYLRKGQSYATTKSIEIQKNQNNYFKFIQKKDI
SLNELDAILMRKDPPFNTEFIYATYILERAEEKGVLVINKPKSLRDCNEKIFISWFSRFTTDTLVTRKLSKIHNFWKEKN
DIILKPLDAMGGKGVFRIKKDDPNFSVIVETLTNYEKKYCMIQTYLPEVQFGDKRILIVNGKPIPWSLTRIPKHGETRAN
LAVGGEGRVQKLNDKDWEIANYLAPILKKRGLILVGLDVIGDKLTEINVTSPTCICEIEEKKNISITGILIDYIEDKIYK
>Mature_320_residues
MRKKKNLKIGIVMDSITLINIKKDSSFAILLEAQKRQHEIYYMEMNDLYLRKGQSYATTKSIEIQKNQNNYFKFIQKKDI
SLNELDAILMRKDPPFNTEFIYATYILERAEEKGVLVINKPKSLRDCNEKIFISWFSRFTTDTLVTRKLSKIHNFWKEKN
DIILKPLDAMGGKGVFRIKKDDPNFSVIVETLTNYEKKYCMIQTYLPEVQFGDKRILIVNGKPIPWSLTRIPKHGETRAN
LAVGGEGRVQKLNDKDWEIANYLAPILKKRGLILVGLDVIGDKLTEINVTSPTCICEIEEKKNISITGILIDYIEDKIYK

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1789316, Length=314, Percent_Identity=59.2356687898089, Blast_Score=415, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSHB_BUCAP (Q8K931)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660859.1
- ProteinModelPortal:   Q8K931
- SMR:   Q8K931
- EnsemblBacteria:   EBBUCT00000000077
- GeneID:   1005649
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg529
- GeneTree:   EBGT00050000007955
- HOGENOM:   HBG624526
- OMA:   WMRKDPP
- ProtClustDB:   PRK05246
- BioCyc:   BAPH198804:BUSG529-MONOMER
- HAMAP:   MF_00162
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01380

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.3

Molecular weight: Translated: 37167; Mature: 37167

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKKNLKIGIVMDSITLINIKKDSSFAILLEAQKRQHEIYYMEMNDLYLRKGQSYATTK
CCCCCCCEEEEEEEEEEEEEEECCCCEEEEEEECCCCCEEEEEEECCEEEECCCCEEEEE
SIEIQKNQNNYFKFIQKKDISLNELDAILMRKDPPFNTEFIYATYILERAEEKGVLVINK
EEEEEECCCCEEEEEEECCCCHHHHHEEEEECCCCCCCEEEEEEEHHHHHCCCCEEEEEC
PKSLRDCNEKIFISWFSRFTTDTLVTRKLSKIHNFWKEKNDIILKPLDAMGGKGVFRIKK
CCHHHHCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCEEEEEE
DDPNFSVIVETLTNYEKKYCMIQTYLPEVQFGDKRILIVNGKPIPWSLTRIPKHGETRAN
CCCCCEEEEEEHHCCCCCEEEEEEECCCCEECCEEEEEECCCCCCEEHEECCCCCCCCEE
LAVGGEGRVQKLNDKDWEIANYLAPILKKRGLILVGLDVIGDKLTEINVTSPTCICEIEE
EEECCCCCEEECCCCCCHHHHHHHHHHHHCCEEEEEHHHHCCCEEEEECCCCEEEEEECC
KKNISITGILIDYIEDKIYK
CCCEEEEEEEEEHHHHHHCC
>Mature Secondary Structure
MRKKKNLKIGIVMDSITLINIKKDSSFAILLEAQKRQHEIYYMEMNDLYLRKGQSYATTK
CCCCCCCEEEEEEEEEEEEEEECCCCEEEEEEECCCCCEEEEEEECCEEEECCCCEEEEE
SIEIQKNQNNYFKFIQKKDISLNELDAILMRKDPPFNTEFIYATYILERAEEKGVLVINK
EEEEEECCCCEEEEEEECCCCHHHHHEEEEECCCCCCCEEEEEEEHHHHHCCCCEEEEEC
PKSLRDCNEKIFISWFSRFTTDTLVTRKLSKIHNFWKEKNDIILKPLDAMGGKGVFRIKK
CCHHHHCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCEEEEEE
DDPNFSVIVETLTNYEKKYCMIQTYLPEVQFGDKRILIVNGKPIPWSLTRIPKHGETRAN
CCCCCEEEEEEHHCCCCCEEEEEEECCCCEECCEEEEEECCCCCCEEHEECCCCCCCCEE
LAVGGEGRVQKLNDKDWEIANYLAPILKKRGLILVGLDVIGDKLTEINVTSPTCICEIEE
EEECCCCCEEECCCCCCHHHHHHHHHHHHCCEEEEEHHHHCCCEEEEECCCCEEEEEECC
KKNISITGILIDYIEDKIYK
CCCEEEEEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438