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The map label for this gene is dnaB

Identifier: 21672791

GI number: 21672791

Start: 583185

End: 584582

Strand: Direct

Name: dnaB

Synonym: BUsg528

Alternate gene names: 21672791

Gene position: 583185-584582 (Clockwise)

Preceding gene: 21672781

Following gene: 21672792

Centisome position: 90.92

GC content: 30.76

Gene sequence:

>1398_bases
ATGGTATATCCAATATATATGGCAAAAAATAAATCATATTTACATCAAATTAATAGATTAAAAATTCCACCACATTCATT
AGAAGCAGAACAATCAGTATTAGGTGGATTAATGTTAGATAATGAACAATGGGATTCTGTTTCAGAACACGTAGTAGCTG
ATGATTTTTTTAGTAAACCACATCGTTTAATATTTCAAGAGATGCAACAATTATTAGATTTAGGACATCCTATTGATTTA
ATTACATTATCAGAATCGTTAGAACAAAAGGGAAAATTAGAAAGTGTAGGTAGATTTTCTTATTTAGCAGAATTATCAAA
AAACACTCCTAGCACAGCAAATATTATAGCATATGCTGATATAATACGCGAAAGAGCAATAGTAAGGGAAATGATATTAG
TAGCCAACAAAATAGCAAATGCTGGATATGATACTCAAGGGAGAAAAAGCGAAGAATTATTAGATTATGCAGAATCTAGT
GTATTTAAAATTGCCGAAAAACGTTTTAAAAAAGATTCAGGTCCCAAAAATATTGAACAAATTCTCGATGAAACTGTTAC
TAGTATTGAAAAATTATTTTTATCTCCTAATGATGGAGTAACAGGAATAAATACAGGATATCAAGATTTAAATAAAAAAA
CATCAGGTTTGCAGCCTTCAGAGCTTATTATTATTGCAGCAAGACCTTCAATGGGTAAAACAACTTTTGCAATGAACTTA
TGTGAAAATGCTGCTATGCTATATGATAAACCAGTATTAATTTTTAGTTTAGAAATGCCTGGAGAGCAAATCATGATGCG
TATGTTAGCATCTTTATCCAGAGTTAATCAGGCACGTATTCGAACAGGACAATTAAATGATGAAGATTGGTCTCGCATGT
CTGGTACTATCAATGTTTTACTTAAAAAAAAGAATATTTATATAGATGATTCTTCAGCTCTTACTCCTAGTGAAGTTCGT
TCTAGAGCACGTCGTATTTATCGTGAAAATAAGGGATTAACTTTAATAATGGTTGATTATTTACAACTAATGAGAGTTCC
ATCTCTTTCTGATAACAGAACTCTTGAAATTGCTGAAATTTCAAGAACTTTAAAAGCATTGGCAAAAGAATTACAAGTAC
CAGTTATAGCATTATCACAATTGAATCGTTCTTTAGAACAAAGATCAGATAAAAGACCAGTAAATTCAGATTTACGCGAA
TCTGGTTCTCTAGAACAAGATGCAGATTTAATAATGTTTATATATCGCGACGAAATATATCACGAAAACAGTGATTTTAA
AGGTGTGGCAGAGATTATAATAGGGAAACAAAGAAATGGACCAATTGGGACAATATGCTTAACATTTAACGGACACTGGT
CTAGATTTGATAATTATTGTGGTCCTAAATATGATTGA

Upstream 100 bases:

>100_bases
TCGATTTCTTAAAATAAGATAAAAAACTAAATTGGATTAGTGAATAAATAGTCTACTATTTAAAATACTATAATATAATT
TTACAATGATTCACTAAATA

Downstream 100 bases:

>100_bases
ATAATACAATAATTTTAGATTAAGATACCAATCTTATTCAAATTAATTTTTATTTTTAAAATTTAACAACTAATTTTAAT
TAAGAAAATTGTAATTTTAA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MVYPIYMAKNKSYLHQINRLKIPPHSLEAEQSVLGGLMLDNEQWDSVSEHVVADDFFSKPHRLIFQEMQQLLDLGHPIDL
ITLSESLEQKGKLESVGRFSYLAELSKNTPSTANIIAYADIIRERAIVREMILVANKIANAGYDTQGRKSEELLDYAESS
VFKIAEKRFKKDSGPKNIEQILDETVTSIEKLFLSPNDGVTGINTGYQDLNKKTSGLQPSELIIIAARPSMGKTTFAMNL
CENAAMLYDKPVLIFSLEMPGEQIMMRMLASLSRVNQARIRTGQLNDEDWSRMSGTINVLLKKKNIYIDDSSALTPSEVR
SRARRIYRENKGLTLIMVDYLQLMRVPSLSDNRTLEIAEISRTLKALAKELQVPVIALSQLNRSLEQRSDKRPVNSDLRE
SGSLEQDADLIMFIYRDEIYHENSDFKGVAEIIIGKQRNGPIGTICLTFNGHWSRFDNYCGPKYD

Sequences:

>Translated_465_residues
MVYPIYMAKNKSYLHQINRLKIPPHSLEAEQSVLGGLMLDNEQWDSVSEHVVADDFFSKPHRLIFQEMQQLLDLGHPIDL
ITLSESLEQKGKLESVGRFSYLAELSKNTPSTANIIAYADIIRERAIVREMILVANKIANAGYDTQGRKSEELLDYAESS
VFKIAEKRFKKDSGPKNIEQILDETVTSIEKLFLSPNDGVTGINTGYQDLNKKTSGLQPSELIIIAARPSMGKTTFAMNL
CENAAMLYDKPVLIFSLEMPGEQIMMRMLASLSRVNQARIRTGQLNDEDWSRMSGTINVLLKKKNIYIDDSSALTPSEVR
SRARRIYRENKGLTLIMVDYLQLMRVPSLSDNRTLEIAEISRTLKALAKELQVPVIALSQLNRSLEQRSDKRPVNSDLRE
SGSLEQDADLIMFIYRDEIYHENSDFKGVAEIIIGKQRNGPIGTICLTFNGHWSRFDNYCGPKYD
>Mature_465_residues
MVYPIYMAKNKSYLHQINRLKIPPHSLEAEQSVLGGLMLDNEQWDSVSEHVVADDFFSKPHRLIFQEMQQLLDLGHPIDL
ITLSESLEQKGKLESVGRFSYLAELSKNTPSTANIIAYADIIRERAIVREMILVANKIANAGYDTQGRKSEELLDYAESS
VFKIAEKRFKKDSGPKNIEQILDETVTSIEKLFLSPNDGVTGINTGYQDLNKKTSGLQPSELIIIAARPSMGKTTFAMNL
CENAAMLYDKPVLIFSLEMPGEQIMMRMLASLSRVNQARIRTGQLNDEDWSRMSGTINVLLKKKNIYIDDSSALTPSEVR
SRARRIYRENKGLTLIMVDYLQLMRVPSLSDNRTLEIAEISRTLKALAKELQVPVIALSQLNRSLEQRSDKRPVNSDLRE
SGSLEQDADLIMFIYRDEIYHENSDFKGVAEIIIGKQRNGPIGTICLTFNGHWSRFDNYCGPKYD

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain

Homologues:

Organism=Escherichia coli, GI1790486, Length=450, Percent_Identity=78.6666666666667, Blast_Score=749, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAB_BUCAP (Q8K932)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660858.1
- ProteinModelPortal:   Q8K932
- SMR:   Q8K932
- EnsemblBacteria:   EBBUCT00000000021
- GeneID:   1005472
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg528
- GeneTree:   EBGT00050000008138
- HOGENOM:   HBG734566
- OMA:   TGYTDLD
- ProtClustDB:   CLSK316468
- BioCyc:   BAPH198804:BUSG528-MONOMER
- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- Gene3D:   G3DSA:1.10.860.10
- SMART:   SM00382
- TIGRFAMs:   TIGR00665

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C; SSF48024 DNA_helicase_DnaB-like_N

EC number: =3.6.4.12

Molecular weight: Translated: 52722; Mature: 52722

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS51199 SF4_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYPIYMAKNKSYLHQINRLKIPPHSLEAEQSVLGGLMLDNEQWDSVSEHVVADDFFSKP
CEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHCCCEECCCCHHHHHHHHHHHHHHCCH
HRLIFQEMQQLLDLGHPIDLITLSESLEQKGKLESVGRFSYLAELSKNTPSTANIIAYAD
HHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IIRERAIVREMILVANKIANAGYDTQGRKSEELLDYAESSVFKIAEKRFKKDSGPKNIEQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ILDETVTSIEKLFLSPNDGVTGINTGYQDLNKKTSGLQPSELIIIAARPSMGKTTFAMNL
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHH
CENAAMLYDKPVLIFSLEMPGEQIMMRMLASLSRVNQARIRTGQLNDEDWSRMSGTINVL
HHCCHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEE
LKKKNIYIDDSSALTPSEVRSRARRIYRENKGLTLIMVDYLQLMRVPSLSDNRTLEIAEI
EEECEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCEEEHHHH
SRTLKALAKELQVPVIALSQLNRSLEQRSDKRPVNSDLRESGSLEQDADLIMFIYRDEIY
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEEEHHHH
HENSDFKGVAEIIIGKQRNGPIGTICLTFNGHWSRFDNYCGPKYD
CCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHCCCCCCCC
>Mature Secondary Structure
MVYPIYMAKNKSYLHQINRLKIPPHSLEAEQSVLGGLMLDNEQWDSVSEHVVADDFFSKP
CEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHCCCEECCCCHHHHHHHHHHHHHHCCH
HRLIFQEMQQLLDLGHPIDLITLSESLEQKGKLESVGRFSYLAELSKNTPSTANIIAYAD
HHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IIRERAIVREMILVANKIANAGYDTQGRKSEELLDYAESSVFKIAEKRFKKDSGPKNIEQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ILDETVTSIEKLFLSPNDGVTGINTGYQDLNKKTSGLQPSELIIIAARPSMGKTTFAMNL
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHH
CENAAMLYDKPVLIFSLEMPGEQIMMRMLASLSRVNQARIRTGQLNDEDWSRMSGTINVL
HHCCHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEE
LKKKNIYIDDSSALTPSEVRSRARRIYRENKGLTLIMVDYLQLMRVPSLSDNRTLEIAEI
EEECEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCEEEHHHH
SRTLKALAKELQVPVIALSQLNRSLEQRSDKRPVNSDLRESGSLEQDADLIMFIYRDEIY
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEEEHHHH
HENSDFKGVAEIIIGKQRNGPIGTICLTFNGHWSRFDNYCGPKYD
CCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438