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The map label for this gene is yheN

Identifier: 21672778

GI number: 21672778

Start: 566485

End: 566871

Strand: Reverse

Name: yheN

Synonym: BUsg513

Alternate gene names: 21672778

Gene position: 566871-566485 (Counterclockwise)

Preceding gene: 21672779

Following gene: 30410825

Centisome position: 88.37

GC content: 28.42

Gene sequence:

>387_bases
ATGAATTATACAGTTTTAGTTACAGGTTCCGCTTATGGAACACAAAATGCCAGTACAGCCTTCCTATTCTGCCAATCATT
GATAAAGATGTCTCATATATTAAATAGTGTTTTCTTTTACTGTGATGGAGTTCTTAATGCTAATAATTTTAATAAACCAT
CTGTCGACGAATTTGATCTACTTTCTGCATGGAAACAATTAAATGAAAAAAATAAGGTAAAATTATATGTTTGTGTTAGT
GCTGCTTCAAGACGAGGTGTCATAGAAGATGAAAAAATATCAAACACAAAAATTAAAAATGGTAATTTTGCTTCTTTTTT
TCAGTCAAGCGGATTATTAGAATTAGCATATTCTATAAAAATTTCTGATCGTATAATACAATTTTAA

Upstream 100 bases:

>100_bases
TATTAGATATCAAAAATATTTAATATTAGTATTCTTTTAACATTTATAGAAATTATTTTTTAGTTCAAATAATTTTAATT
TTGAATCAAGATATTTTAAT

Downstream 100 bases:

>100_bases
TATGTTTATTTCAATGGAAAAAATTGCTTTTGTTTTTTCTCATGCGCCGCATGGGACTAGTTTTGGTAAAGAAGGATTGG
ATGTTATTTTAGGTGTTTCT

Product: sulfur transfer complex subunit TusD

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein D

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MNYTVLVTGSAYGTQNASTAFLFCQSLIKMSHILNSVFFYCDGVLNANNFNKPSVDEFDLLSAWKQLNEKNKVKLYVCVS
AASRRGVIEDEKISNTKIKNGNFASFFQSSGLLELAYSIKISDRIIQF

Sequences:

>Translated_128_residues
MNYTVLVTGSAYGTQNASTAFLFCQSLIKMSHILNSVFFYCDGVLNANNFNKPSVDEFDLLSAWKQLNEKNKVKLYVCVS
AASRRGVIEDEKISNTKIKNGNFASFFQSSGLLELAYSIKISDRIIQF
>Mature_128_residues
MNYTVLVTGSAYGTQNASTAFLFCQSLIKMSHILNSVFFYCDGVLNANNFNKPSVDEFDLLSAWKQLNEKNKVKLYVCVS
AASRRGVIEDEKISNTKIKNGNFASFFQSSGLLELAYSIKISDRIIQF

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Accepts sulfur from tusA and transfers it in turn to tusE

COG id: COG1553

COG function: function code P; Uncharacterized conserved protein involved in intracellular sulfur reduction

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrE/tusD family

Homologues:

Organism=Escherichia coli, GI1789743, Length=128, Percent_Identity=47.65625, Blast_Score=130, Evalue=3e-32,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): TUSD_BUCAP (Q8K944)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660845.1
- ProteinModelPortal:   Q8K944
- SMR:   Q8K944
- EnsemblBacteria:   EBBUCT00000000312
- GeneID:   1005658
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg513
- GeneTree:   EBGT00050000008175
- HOGENOM:   HBG538485
- OMA:   QDGVYNS
- ProtClustDB:   PRK00207
- BioCyc:   BAPH198804:BUSG513-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00390
- InterPro:   IPR003787
- InterPro:   IPR017463
- TIGRFAMs:   TIGR03012

Pfam domain/function: PF02635 DrsE

EC number: NA

Molecular weight: Translated: 14342; Mature: 14342

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: ACT_SITE 78-78

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTVLVTGSAYGTQNASTAFLFCQSLIKMSHILNSVFFYCDGVLNANNFNKPSVDEFDL
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
LSAWKQLNEKNKVKLYVCVSAASRRGVIEDEKISNTKIKNGNFASFFQSSGLLELAYSIK
HHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCCHHHHHHCCCEEEEEEEEE
ISDRIIQF
ECCEEECC
>Mature Secondary Structure
MNYTVLVTGSAYGTQNASTAFLFCQSLIKMSHILNSVFFYCDGVLNANNFNKPSVDEFDL
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
LSAWKQLNEKNKVKLYVCVSAASRRGVIEDEKISNTKIKNGNFASFFQSSGLLELAYSIK
HHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCCHHHHHHCCCEEEEEEEEE
ISDRIIQF
ECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438