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The map label for this gene is aroE

Identifier: 21672739

GI number: 21672739

Start: 543669

End: 544490

Strand: Reverse

Name: aroE

Synonym: BUsg474

Alternate gene names: 21672739

Gene position: 544490-543669 (Counterclockwise)

Preceding gene: 21672740

Following gene: 21672738

Centisome position: 84.88

GC content: 25.06

Gene sequence:

>822_bases
ATGTGTAAACTTGAAAAGTTTAATTACGCTTTGTTTGGAAATCCTATTGATCACAGTCAATCTCCTAAAATTCATAATTT
TTTTGCTACACAAACAGGTATTTTGCATATTTATAAAGCTATTAACATTCCATTAGATCAATTTTCTTCAGTAGTTTCAG
ATTTTTTTAAAAAAAATATTAAAGGTGCTAATGTAACAGCACCTTTTAAAAAAGAAGCGTATTTTTTTTCTGATAAGTTA
ACTGAAAGAGCGAAGATTGCACAATCTGTAAATACTTTAAAGAAAATAAGCGATAAATGTATTTTAGGAGATAATACTGA
CGGTATAGGATTATTATCTGATTTAGTTAGATTAAATTTTATAAAAAAAAATTTTTCTATATTAATACTTGGTGCAGGAG
GTGCTGTGAAAGGTGTTCTTTTGCCACTTTTATCTTTGGGATGTTCAGTTTACATTTTAAATCGAACTATTTTAAATGCT
AAAATTTTAGTTAAACAATTTAATAAATATGGAAAAATTTTTGTTTTTGATAGACAAAATTTTAAACAACAAAATTTTGA
TTTAGTTATCAATGCTATGTCAAGAAATACTGAGAAAAAAAATTTTTTACCCTTATCTTTAATTACTTCTAAAACTTTTT
TTTACGATATGAATTATAGTACACGCAATACACCCTTTATTAATTGGTGTTCTAAAGCAGGAGGAAGCTTTATTTCTAAT
GGAATAGGGATGCTGGTTTTTCAAGCAGCTTATTCTTTTTTAGAATGGCACAATGTACTTCCAGAAATAAATTATATTAT
TAATATTTTAAATATAAAATAA

Upstream 100 bases:

>100_bases
AAGGACTTTGGAAAAGATTTTCCAGTATTGTATGGTCATATAGGAAATGAAAGTCATCCCTCTAAAATAATTAATATTGT
TAATGGAAAATTAATTCGTT

Downstream 100 bases:

>100_bases
TTTTTTTAAAAAATGTTTAATATTTATTTTTTTTATATATTCTCTTCTTAATTTTAAAGAAAATACTTGACTCTTTTTTT
TCGATCTGTATTATTTAATG

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MCKLEKFNYALFGNPIDHSQSPKIHNFFATQTGILHIYKAINIPLDQFSSVVSDFFKKNIKGANVTAPFKKEAYFFSDKL
TERAKIAQSVNTLKKISDKCILGDNTDGIGLLSDLVRLNFIKKNFSILILGAGGAVKGVLLPLLSLGCSVYILNRTILNA
KILVKQFNKYGKIFVFDRQNFKQQNFDLVINAMSRNTEKKNFLPLSLITSKTFFYDMNYSTRNTPFINWCSKAGGSFISN
GIGMLVFQAAYSFLEWHNVLPEINYIINILNIK

Sequences:

>Translated_273_residues
MCKLEKFNYALFGNPIDHSQSPKIHNFFATQTGILHIYKAINIPLDQFSSVVSDFFKKNIKGANVTAPFKKEAYFFSDKL
TERAKIAQSVNTLKKISDKCILGDNTDGIGLLSDLVRLNFIKKNFSILILGAGGAVKGVLLPLLSLGCSVYILNRTILNA
KILVKQFNKYGKIFVFDRQNFKQQNFDLVINAMSRNTEKKNFLPLSLITSKTFFYDMNYSTRNTPFINWCSKAGGSFISN
GIGMLVFQAAYSFLEWHNVLPEINYIINILNIK
>Mature_273_residues
MCKLEKFNYALFGNPIDHSQSPKIHNFFATQTGILHIYKAINIPLDQFSSVVSDFFKKNIKGANVTAPFKKEAYFFSDKL
TERAKIAQSVNTLKKISDKCILGDNTDGIGLLSDLVRLNFIKKNFSILILGAGGAVKGVLLPLLSLGCSVYILNRTILNA
KILVKQFNKYGKIFVFDRQNFKQQNFDLVINAMSRNTEKKNFLPLSLITSKTFFYDMNYSTRNTPFINWCSKAGGSFISN
GIGMLVFQAAYSFLEWHNVLPEINYIINILNIK

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=265, Percent_Identity=48.6792452830189, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1787983, Length=277, Percent_Identity=24.5487364620939, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320332, Length=188, Percent_Identity=33.5106382978723, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_BUCAP (P46240)

Other databases:

- EMBL:   U09230
- EMBL:   AE013218
- RefSeq:   NP_660806.1
- ProteinModelPortal:   P46240
- SMR:   P46240
- EnsemblBacteria:   EBBUCT00000000257
- GeneID:   1005693
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg474
- GeneTree:   EBGT00050000008051
- HOGENOM:   HBG553408
- OMA:   DQYVVFG
- ProtClustDB:   PRK00258
- BioCyc:   BAPH198804:BUSG474-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 30885; Mature: 30885

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: ACT_SITE 70-70

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKLEKFNYALFGNPIDHSQSPKIHNFFATQTGILHIYKAINIPLDQFSSVVSDFFKKNI
CCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
KGANVTAPFKKEAYFFSDKLTERAKIAQSVNTLKKISDKCILGDNTDGIGLLSDLVRLNF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHH
IKKNFSILILGAGGAVKGVLLPLLSLGCSVYILNRTILNAKILVKQFNKYGKIFVFDRQN
HCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCC
FKQQNFDLVINAMSRNTEKKNFLPLSLITSKTFFYDMNYSTRNTPFINWCSKAGGSFISN
CCCCCHHEEEEEHHCCCCCCCCEEEEEEECEEEEEEECCCCCCCCCEEHHHHCCCHHHHC
GIGMLVFQAAYSFLEWHNVLPEINYIINILNIK
CHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECC
>Mature Secondary Structure
MCKLEKFNYALFGNPIDHSQSPKIHNFFATQTGILHIYKAINIPLDQFSSVVSDFFKKNI
CCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
KGANVTAPFKKEAYFFSDKLTERAKIAQSVNTLKKISDKCILGDNTDGIGLLSDLVRLNF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHH
IKKNFSILILGAGGAVKGVLLPLLSLGCSVYILNRTILNAKILVKQFNKYGKIFVFDRQN
HCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCC
FKQQNFDLVINAMSRNTEKKNFLPLSLITSKTFFYDMNYSTRNTPFINWCSKAGGSFISN
CCCCCHHEEEEEHHCCCCCCCCEEEEEEECEEEEEEECCCCCCCCCEEHHHHCCCHHHHC
GIGMLVFQAAYSFLEWHNVLPEINYIINILNIK
CHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7535281; 12089438