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The map label for this gene is ribH

Identifier: 21672708

GI number: 21672708

Start: 506724

End: 507203

Strand: Direct

Name: ribH

Synonym: BUsg443

Alternate gene names: 21672708

Gene position: 506724-507203 (Clockwise)

Preceding gene: 21672707

Following gene: 21672709

Centisome position: 79.0

GC content: 25.0

Gene sequence:

>480_bases
ATGAATATAATTCAATCAGGAATCAACGTTAAAAATGCTTCTATTACTATAATAATAGCAAGATTTAATGAATTTATTAA
TAAAAATTTATTATCTGGAGCAATAGACACACTAACAAGAATAGGTCAAATAGACAAAGAGAAAATTTTAACAATATATG
TTCCTGGAACTTATGAAATACCTATAGTAGCAAGTTATATTGCAAATACTAATAAATATGACGCTATAATTGCAATTGGT
ACAATTATTAAAGGTAGTACTGATCATTTTAAACATATTGCGAATGATGCTTATAGTAATCTTTCGAGAATTAGCACAAA
ATATTTTATACCTATTACAATGGGAATATTAACTACAGAAAACATTCAACAATCTATTGAAAGGTCTGGTACAAAAATGG
GTAATAAGGGATCTGAAGCTGCATTAGCTGCATTAGAAATGATTAATATTATGAAAAAATTAAAAAAAATAAATAATTAA

Upstream 100 bases:

>100_bases
CGGAGAAAAAGCTGGTAAATTGGCAGGTTTTTATAACCATTATGGATACGCTTGGGTACTGAAAATTTAACTCAAAAAAC
ATAAACAAAAAGGAAAAAAA

Downstream 100 bases:

>100_bases
ATTATGGAATATAACGAATTTGAAATTATATCTAAATTTTTTAAAAATCATCAAAAAAAAGATAAAAATCAAATTAAGGG
AATTGGAGACGATAGTGCCT

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNIIQSGINVKNASITIIIARFNEFINKNLLSGAIDTLTRIGQIDKEKILTIYVPGTYEIPIVASYIANTNKYDAIIAIG
TIIKGSTDHFKHIANDAYSNLSRISTKYFIPITMGILTTENIQQSIERSGTKMGNKGSEAALAALEMINIMKKLKKINN

Sequences:

>Translated_159_residues
MNIIQSGINVKNASITIIIARFNEFINKNLLSGAIDTLTRIGQIDKEKILTIYVPGTYEIPIVASYIANTNKYDAIIAIG
TIIKGSTDHFKHIANDAYSNLSRISTKYFIPITMGILTTENIQQSIERSGTKMGNKGSEAALAALEMINIMKKLKKINN
>Mature_159_residues
MNIIQSGINVKNASITIIIARFNEFINKNLLSGAIDTLTRIGQIDKEKILTIYVPGTYEIPIVASYIANTNKYDAIIAIG
TIIKGSTDHFKHIANDAYSNLSRISTKYFIPITMGILTTENIQQSIERSGTKMGNKGSEAALAALEMINIMKKLKKINN

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=155, Percent_Identity=53.5483870967742, Blast_Score=187, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6324429, Length=142, Percent_Identity=32.3943661971831, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_BUCAP (Q8K9A6)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660775.1
- ProteinModelPortal:   Q8K9A6
- SMR:   Q8K9A6
- EnsemblBacteria:   EBBUCT00000000120
- GeneID:   1005721
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg443
- GeneTree:   EBGT00050000008162
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- BioCyc:   BAPH198804:BUSG443-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 17521; Mature: 17521

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIQSGINVKNASITIIIARFNEFINKNLLSGAIDTLTRIGQIDKEKILTIYVPGTYEI
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCH
PIVASYIANTNKYDAIIAIGTIIKGSTDHFKHIANDAYSNLSRISTKYFIPITMGILTTE
HHHHHHHCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHH
NIQQSIERSGTKMGNKGSEAALAALEMINIMKKLKKINN
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIIQSGINVKNASITIIIARFNEFINKNLLSGAIDTLTRIGQIDKEKILTIYVPGTYEI
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCH
PIVASYIANTNKYDAIIAIGTIIKGSTDHFKHIANDAYSNLSRISTKYFIPITMGILTTE
HHHHHHHCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHH
NIQQSIERSGTKMGNKGSEAALAALEMINIMKKLKKINN
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438