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The map label for this gene is ispD

Identifier: 21672677

GI number: 21672677

Start: 457661

End: 458371

Strand: Reverse

Name: ispD

Synonym: BUsg405

Alternate gene names: 21672677

Gene position: 458371-457661 (Counterclockwise)

Preceding gene: 21672678

Following gene: 21672676

Centisome position: 71.46

GC content: 26.86

Gene sequence:

>711_bases
ATGAAATTAATTAATTCACCTAAACTAAAAATTATAGCTATAGTACCAGCTGCTGGAATAGGTAGAAGGATGAAATTAGA
TTTTCCAAAACAATATATAAAAATAAAAGATTGTACTATTCTTGAATATACTTTAAAAACATTATTATCGCATCCTAATA
TAGTTCGTATTGTGGTAAGTTTGCATCAAGAAGATAATTTTTTTCAAAAATTATCTATATCATCTGATTTACGTGTTTTT
TCTGTATTAGGCGGTAACGAAAGAATACATTCAGTTTTATCTGGTTTAATTATAACAACAGATGCTAAATGGGTGATAAT
TCATGATGCAGTTCGTCCTTGTTTAAGTTATCAAGATTTAGAAAATTTAATTGCTATCACTAAAAATACTAAAGTAGGTG
GAATTTTAGCACGACCTGTATGTGATACTATCAAATATAGTAATCGAAAAAATAAAACAATATTACATACTATTCCCAGA
AATCAGTTGTGGCATGCTTTAACTCCTCAGTTGTTTCCGATCAATTTATTACGGTTTTGTTTAAAGAAGATTGTTGAAGA
TAAAATAAATATAACAGATGAAGCTTCAGCATTAGAATATTGCGGATATCATCCATTAATTGTTTTAGGAAGTTACAAAA
ATATTAAGATAACTTATCCTGAAGATCTCATCTTTGCAGAATTTTATCTTAAAGAACTATTAAAAAATTAA

Upstream 100 bases:

>100_bases
AATATTAGAAATTGAACGTTTAAATAATGCAATTAAAAATTAAAAAACAATATCTATTTTTTAAATATAATGAATAAAAA
TTATTTTTTTTGGATATTAC

Downstream 100 bases:

>100_bases
GAGGATGTATCGATAATGAGAATTGGATACGGTTTTGATATTCATGCTTTCGGTAGTATAAAACCTTTGATCATTGGAGG
TGTTCAAATACCTTATAATA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKLINSPKLKIIAIVPAAGIGRRMKLDFPKQYIKIKDCTILEYTLKTLLSHPNIVRIVVSLHQEDNFFQKLSISSDLRVF
SVLGGNERIHSVLSGLIITTDAKWVIIHDAVRPCLSYQDLENLIAITKNTKVGGILARPVCDTIKYSNRKNKTILHTIPR
NQLWHALTPQLFPINLLRFCLKKIVEDKINITDEASALEYCGYHPLIVLGSYKNIKITYPEDLIFAEFYLKELLKN

Sequences:

>Translated_236_residues
MKLINSPKLKIIAIVPAAGIGRRMKLDFPKQYIKIKDCTILEYTLKTLLSHPNIVRIVVSLHQEDNFFQKLSISSDLRVF
SVLGGNERIHSVLSGLIITTDAKWVIIHDAVRPCLSYQDLENLIAITKNTKVGGILARPVCDTIKYSNRKNKTILHTIPR
NQLWHALTPQLFPINLLRFCLKKIVEDKINITDEASALEYCGYHPLIVLGSYKNIKITYPEDLIFAEFYLKELLKN
>Mature_236_residues
MKLINSPKLKIIAIVPAAGIGRRMKLDFPKQYIKIKDCTILEYTLKTLLSHPNIVRIVVSLHQEDNFFQKLSISSDLRVF
SVLGGNERIHSVLSGLIITTDAKWVIIHDAVRPCLSYQDLENLIAITKNTKVGGILARPVCDTIKYSNRKNKTILHTIPR
NQLWHALTPQLFPINLLRFCLKKIVEDKINITDEASALEYCGYHPLIVLGSYKNIKITYPEDLIFAEFYLKELLKN

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=235, Percent_Identity=28.0851063829787, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1789104, Length=222, Percent_Identity=50.4504504504504, Blast_Score=248, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_BUCAP (Q8K9D6)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660744.1
- ProteinModelPortal:   Q8K9D6
- SMR:   Q8K9D6
- EnsemblBacteria:   EBBUCT00000000196
- GeneID:   1005755
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg405
- GeneTree:   EBGT00050000007877
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- ProtClustDB:   PRK00155
- BioCyc:   BAPH198804:BUSG405-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26967; Mature: 26967

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLINSPKLKIIAIVPAAGIGRRMKLDFPKQYIKIKDCTILEYTLKTLLSHPNIVRIVVS
CCCCCCCCEEEEEEECCCCCCCEEEECCCHHHEEECCCCHHHHHHHHHHCCCCEEEEEEE
LHQEDNFFQKLSISSDLRVFSVLGGNERIHSVLSGLIITTDAKWVIIHDAVRPCLSYQDL
HHCCCCHHHHHCCCCCCEEEEEECCCHHHHHHHHCEEEECCCCEEEEEHHHHHHHCHHHH
ENLIAITKNTKVGGILARPVCDTIKYSNRKNKTILHTIPRNQLWHALTPQLFPINLLRFC
HHHEEEECCCCCCCEEHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCCHHHHHHHH
LKKIVEDKINITDEASALEYCGYHPLIVLGSYKNIKITYPEDLIFAEFYLKELLKN
HHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLINSPKLKIIAIVPAAGIGRRMKLDFPKQYIKIKDCTILEYTLKTLLSHPNIVRIVVS
CCCCCCCCEEEEEEECCCCCCCEEEECCCHHHEEECCCCHHHHHHHHHHCCCCEEEEEEE
LHQEDNFFQKLSISSDLRVFSVLGGNERIHSVLSGLIITTDAKWVIIHDAVRPCLSYQDL
HHCCCCHHHHHCCCCCCEEEEEECCCHHHHHHHHCEEEECCCCEEEEEHHHHHHHCHHHH
ENLIAITKNTKVGGILARPVCDTIKYSNRKNKTILHTIPRNQLWHALTPQLFPINLLRFC
HHHEEEECCCCCCCEEHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCCHHHHHHHH
LKKIVEDKINITDEASALEYCGYHPLIVLGSYKNIKITYPEDLIFAEFYLKELLKN
HHHHHHHHCCCCCCHHHHHHCCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438