Click here to switch to the map view.

The map label for this gene is ispF

Identifier: 21672676

GI number: 21672676

Start: 457142

End: 457645

Strand: Reverse

Name: ispF

Synonym: BUsg404

Alternate gene names: 21672676

Gene position: 457645-457142 (Counterclockwise)

Preceding gene: 21672677

Following gene: 21672675

Centisome position: 71.34

GC content: 25.79

Gene sequence:

>504_bases
ATGAGAATTGGATACGGTTTTGATATTCATGCTTTCGGTAGTATAAAACCTTTGATCATTGGAGGTGTTCAAATACCTTA
TAATAAAGGTTTGATCGCTCATTCTAATGGTGATTTACTAATACATTCTTTAATAGATGCATTACTTGGTGCTACTGCTA
TGGGTGATATTGGTACGTTTTTTCCCAGCGAAGATAAAAAATATAAAAATATTAATAGTAGAATTTTATTAAAATATATT
TGGAAAAGAATTTATTTAAAAAACTATCGTATATCTAATATTGATATTACTATTATCACTGAAACTCCTAAGATATTATC
TTATATTTTTCTTATGAGATCAAATATAGCATCAGATCTTAATATTAAAATAGAAAAGATTAGTGTAAAATCTACTAGTT
CTAAAATGATAGGTTGTATCGGAAGAAAAGAAGGAATAGCTTGTCAATCTCTTGTCATGCTTGTAAAATCTAAAAATACT
GATCGAAAAAATAATATAATTTAA

Upstream 100 bases:

>100_bases
AGGAAGTTACAAAAATATTAAGATAACTTATCCTGAAGATCTCATCTTTGCAGAATTTTATCTTAAAGAACTATTAAAAA
ATTAAGAGGATGTATCGATA

Downstream 100 bases:

>100_bases
TATGTATTATTTTTTTTTAAGTATAATATAATGTTTATATTAAATGAAGTTATTACTTGATTTTATTAATTATGAATTAA
ATGGTATAATTTAATGCAAT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MRIGYGFDIHAFGSIKPLIIGGVQIPYNKGLIAHSNGDLLIHSLIDALLGATAMGDIGTFFPSEDKKYKNINSRILLKYI
WKRIYLKNYRISNIDITIITETPKILSYIFLMRSNIASDLNIKIEKISVKSTSSKMIGCIGRKEGIACQSLVMLVKSKNT
DRKNNII

Sequences:

>Translated_167_residues
MRIGYGFDIHAFGSIKPLIIGGVQIPYNKGLIAHSNGDLLIHSLIDALLGATAMGDIGTFFPSEDKKYKNINSRILLKYI
WKRIYLKNYRISNIDITIITETPKILSYIFLMRSNIASDLNIKIEKISVKSTSSKMIGCIGRKEGIACQSLVMLVKSKNT
DRKNNII
>Mature_167_residues
MRIGYGFDIHAFGSIKPLIIGGVQIPYNKGLIAHSNGDLLIHSLIDALLGATAMGDIGTFFPSEDKKYKNINSRILLKYI
WKRIYLKNYRISNIDITIITETPKILSYIFLMRSNIASDLNIKIEKISVKSTSSKMIGCIGRKEGIACQSLVMLVKSKNT
DRKNNII

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=52.2292993630573, Blast_Score=174, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_BUCAP (Q8K9D7)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660743.1
- ProteinModelPortal:   Q8K9D7
- SMR:   Q8K9D7
- EnsemblBacteria:   EBBUCT00000000523
- GeneID:   1005756
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg404
- GeneTree:   EBGT00050000008014
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   BAPH198804:BUSG404-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 18707; Mature: 18707

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGYGFDIHAFGSIKPLIIGGVQIPYNKGLIAHSNGDLLIHSLIDALLGATAMGDIGTF
CCCCCCCCEEECCCCCEEEEECEECCCCCCEEEECCCCEEHHHHHHHHHCCHHCCCCCCC
FPSEDKKYKNINSRILLKYIWKRIYLKNYRISNIDITIITETPKILSYIFLMRSNIASDL
CCCCCCHHHCCCHHHHHHHHHHHHHHCCEEECCEEEEEEECCHHHHHHHHHHHHCCCCCC
NIKIEKISVKSTSSKMIGCIGRKEGIACQSLVMLVKSKNTDRKNNII
EEEEEEEEECCCCCHHEEECCCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRIGYGFDIHAFGSIKPLIIGGVQIPYNKGLIAHSNGDLLIHSLIDALLGATAMGDIGTF
CCCCCCCCEEECCCCCEEEEECEECCCCCCEEEECCCCEEHHHHHHHHHCCHHCCCCCCC
FPSEDKKYKNINSRILLKYIWKRIYLKNYRISNIDITIITETPKILSYIFLMRSNIASDL
CCCCCCHHHCCCHHHHHHHHHHHHHHCCEEECCEEEEEEECCHHHHHHHHHHHHCCCCCC
NIKIEKISVKSTSSKMIGCIGRKEGIACQSLVMLVKSKNTDRKNNII
EEEEEEEEECCCCCHHEEECCCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438