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The map label for this gene is metK

Identifier: 21672667

GI number: 21672667

Start: 445326

End: 446465

Strand: Reverse

Name: metK

Synonym: BUsg393

Alternate gene names: 21672667

Gene position: 446465-445326 (Counterclockwise)

Preceding gene: 21672669

Following gene: 21672662

Centisome position: 69.6

GC content: 32.11

Gene sequence:

>1140_bases
ATGACTGAATATCTTTTTACATCAGAATCAGTTTCAGAAGGACATCCTGATAAAATTGCAGATCAAATTTCTGATGCTTT
ATTAGATGAAATTATTAAACAAGATTTAAAAGCTAGAGTAGCTTGTGAAACTTATGTAAAAACAGGTATGGTTTTAATTG
GAGGTGAAATTACAACTACAGCTTGGGTTGATGTTGAAGAAATTACTAGAAATACTATTAACAATATTGGTTATATTAAT
TCTGAAACAGGTTTTGACGCGAATTCTTGTGCAGTACTTAGTACTATAGGAAAGCAGTCTCCTGATATTACCCAAGGGGT
AGATCGTTGTAATCCCTTAGAACAAGGAGCAGGAGATCAAGGGATTATTTTTGGTTACGCTACTAATGAAACAGAAGTTT
TAATGCCTGCACCTATTACCTATGCTCATTTATTAGTAAAAAAACAATCTGAGTTAAGAAAAAAAAATATTTTACACTGG
CTTAGACCAGATGCTAAAAGCCAAGTAACATTTAAATATAAAAATGGACGTATTATTGGAATAGATACCGTAGTTTTTTC
TACTCAACATAAAGAAAGTATTACGCAGGATGTTTTAAAAGAAGCTGTTATGGAAGAAATTATAAAACCAGTTTTACCAA
ATAAATGGTTAACTAAAAATACAAAATTTTTTATAAATCCTACAGGTAGGTTTGTTATTGGAGGTCCTATGGGAGACTGT
GGTTTAACAGGGCGTAAAATTATTGTTGATACTTATGGTGGAATGTCAAGACATGGAGGAGGTGCTTTTTCTGGTAAAGA
TCCTTCAAAAGTGGATCGTTCTGCTGCCTATGCAGCAAGATATGTAGCTAAAAATATTGTTGCAGCAGGATTAGCAGATC
GTTGTGAAATTCAATTATCATATGCTATTGGAATTGCTGAACCTACTTCAATTATGATAGAAACTTTTAGAACAGGGAAA
ATAAGTAATAAGTCTTTAATTAATTTAGTACGTAACATTTTTGATTTACGTCCTTATGGATTAATTGAAATGCTTGATTT
GCTTCGACCAATTTATCTTAATACAGCAGTATACGGACATTTTGGAAGGGAGGAGTTTCCTTGGGAAAAATTAGATAAAG
TAGACGAATTATTGCAGTAA

Upstream 100 bases:

>100_bases
TTATTTTTAATAATATTCTAAATTAGATAGAAATATTAGCATATCTTTCATTGAAATATTTTTTAATATTTCATAATTTT
AAAAAAAGGTAAATAAAAAA

Downstream 100 bases:

>100_bases
AAATTATTGGTATTTTTTTCTTATAGCAAACAAATAAATTAAATATATTATATGTATTTACTAGTATAGTAAAATGAACT
TAATACGGAAAATTATGTTG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTEYLFTSESVSEGHPDKIADQISDALLDEIIKQDLKARVACETYVKTGMVLIGGEITTTAWVDVEEITRNTINNIGYIN
SETGFDANSCAVLSTIGKQSPDITQGVDRCNPLEQGAGDQGIIFGYATNETEVLMPAPITYAHLLVKKQSELRKKNILHW
LRPDAKSQVTFKYKNGRIIGIDTVVFSTQHKESITQDVLKEAVMEEIIKPVLPNKWLTKNTKFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGMSRHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGIAEPTSIMIETFRTGK
ISNKSLINLVRNIFDLRPYGLIEMLDLLRPIYLNTAVYGHFGREEFPWEKLDKVDELLQ

Sequences:

>Translated_379_residues
MTEYLFTSESVSEGHPDKIADQISDALLDEIIKQDLKARVACETYVKTGMVLIGGEITTTAWVDVEEITRNTINNIGYIN
SETGFDANSCAVLSTIGKQSPDITQGVDRCNPLEQGAGDQGIIFGYATNETEVLMPAPITYAHLLVKKQSELRKKNILHW
LRPDAKSQVTFKYKNGRIIGIDTVVFSTQHKESITQDVLKEAVMEEIIKPVLPNKWLTKNTKFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGMSRHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGIAEPTSIMIETFRTGK
ISNKSLINLVRNIFDLRPYGLIEMLDLLRPIYLNTAVYGHFGREEFPWEKLDKVDELLQ
>Mature_378_residues
TEYLFTSESVSEGHPDKIADQISDALLDEIIKQDLKARVACETYVKTGMVLIGGEITTTAWVDVEEITRNTINNIGYINS
ETGFDANSCAVLSTIGKQSPDITQGVDRCNPLEQGAGDQGIIFGYATNETEVLMPAPITYAHLLVKKQSELRKKNILHWL
RPDAKSQVTFKYKNGRIIGIDTVVFSTQHKESITQDVLKEAVMEEIIKPVLPNKWLTKNTKFFINPTGRFVIGGPMGDCG
LTGRKIIVDTYGGMSRHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGIAEPTSIMIETFRTGKI
SNKSLINLVRNIFDLRPYGLIEMLDLLRPIYLNTAVYGHFGREEFPWEKLDKVDELLQ

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=56.989247311828, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI5174529, Length=372, Percent_Identity=55.6451612903226, Blast_Score=401, Evalue=1e-112,
Organism=Escherichia coli, GI1789311, Length=377, Percent_Identity=79.5755968169761, Blast_Score=644, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538498, Length=374, Percent_Identity=54.8128342245989, Blast_Score=393, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17551082, Length=374, Percent_Identity=54.2780748663102, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538494, Length=374, Percent_Identity=54.2780748663102, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25145633, Length=377, Percent_Identity=53.5809018567639, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565905, Length=374, Percent_Identity=48.9304812834225, Blast_Score=327, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI32565911, Length=327, Percent_Identity=52.5993883792049, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI25145635, Length=330, Percent_Identity=51.2121212121212, Blast_Score=318, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI32565903, Length=300, Percent_Identity=52.3333333333333, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=59.0361445783133, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323209, Length=372, Percent_Identity=57.5268817204301, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6320710, Length=377, Percent_Identity=55.9681697612732, Blast_Score=402, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45552159, Length=372, Percent_Identity=55.1075268817204, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580529, Length=372, Percent_Identity=55.1075268817204, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580537, Length=372, Percent_Identity=55.1075268817204, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580533, Length=372, Percent_Identity=55.1075268817204, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580535, Length=372, Percent_Identity=55.1075268817204, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580531, Length=372, Percent_Identity=55.1075268817204, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580527, Length=373, Percent_Identity=54.9597855227882, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580525, Length=373, Percent_Identity=54.9597855227882, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=52.6737967914438, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=55.4621848739496, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_BUCAP (Q8K9E5)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660734.1
- ProteinModelPortal:   Q8K9E5
- SMR:   Q8K9E5
- EnsemblBacteria:   EBBUCT00000000461
- GeneID:   1005765
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg393
- GeneTree:   EBGT00050000008145
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   BAPH198804:BUSG393-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42004; Mature: 41872

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYLFTSESVSEGHPDKIADQISDALLDEIIKQDLKARVACETYVKTGMVLIGGEITTT
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEE
AWVDVEEITRNTINNIGYINSETGFDANSCAVLSTIGKQSPDITQGVDRCNPLEQGAGDQ
EEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHCCCCC
GIIFGYATNETEVLMPAPITYAHLLVKKQSELRKKNILHWLRPDAKSQVTFKYKNGRIIG
CEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEE
IDTVVFSTQHKESITQDVLKEAVMEEIIKPVLPNKWLTKNTKFFINPTGRFVIGGPMGDC
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGMSRHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGIAEPTSIMIETFRTGKISNKSLINLVRNIFDLRPYGLIEMLDLLRPIYLNTAVYGH
EEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEC
FGREEFPWEKLDKVDELLQ
CCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure 
TEYLFTSESVSEGHPDKIADQISDALLDEIIKQDLKARVACETYVKTGMVLIGGEITTT
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEE
AWVDVEEITRNTINNIGYINSETGFDANSCAVLSTIGKQSPDITQGVDRCNPLEQGAGDQ
EEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHCCCCC
GIIFGYATNETEVLMPAPITYAHLLVKKQSELRKKNILHWLRPDAKSQVTFKYKNGRIIG
CEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEE
IDTVVFSTQHKESITQDVLKEAVMEEIIKPVLPNKWLTKNTKFFINPTGRFVIGGPMGDC
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGMSRHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGIAEPTSIMIETFRTGKISNKSLINLVRNIFDLRPYGLIEMLDLLRPIYLNTAVYGH
EEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEC
FGREEFPWEKLDKVDELLQ
CCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438