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The map label for this gene is greA

Identifier: 21672645

GI number: 21672645

Start: 425415

End: 425894

Strand: Reverse

Name: greA

Synonym: BUsg371

Alternate gene names: 21672645

Gene position: 425894-425415 (Counterclockwise)

Preceding gene: 21672651

Following gene: 21672644

Centisome position: 66.4

GC content: 28.54

Gene sequence:

>480_bases
ATGATTAATTTAATTCCAATGACTGTTAGAGGTGCCGAAAAACTTCGTGAAGAACTTGAACAATTAAAAAATGTAAAACG
TCCTCGCATTACTGTCCAAATAGCAGAAGCTAGAAAGCACGGCGACTTAAAAGAAAATGCTGAATATCATTCAGCACGAG
AAGAACAAAGTTTTTGTGAAGGACGTATACAAGAAATTGAATCCAAATTATCTAATTCTCAAATTATAGATATAACAAAA
ATATCAAATGATGGACGAGTAGTTTTTGGTTCTACAGTAACTATTTTAAATATAAAGAACAATGCAGTGTTTACTTATCA
GATTGTTGGTGATGATGAATCAGATTTTAAAAAAAATTTAATTTCTATTAATTCCCCGATGTCAAGAGGTTTAATTGGTA
AAACTGTTAACACCATTGCTATTATACATACACCATCAGGTAATGTTAAATATAAAATTTTAAAAATAGATTATATTTAA

Upstream 100 bases:

>100_bases
AAATTTATAAAAATAATAGTGTTTTATAAATATTTTTTTATGCTTAAAAAAAGATTCGTTTTATATTTTTTTTAAGAAGT
AACATAATGAGGTATTTTAT

Downstream 100 bases:

>100_bases
ATATATACTTTTTTATAATAAGAAATACAATCTTCTATAATATAAAGATATTTGATAAAAATATGATTTCTAAAAAAAAA
TCAAATAGTTCTAATCGTTG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MINLIPMTVRGAEKLREELEQLKNVKRPRITVQIAEARKHGDLKENAEYHSAREEQSFCEGRIQEIESKLSNSQIIDITK
ISNDGRVVFGSTVTILNIKNNAVFTYQIVGDDESDFKKNLISINSPMSRGLIGKTVNTIAIIHTPSGNVKYKILKIDYI

Sequences:

>Translated_159_residues
MINLIPMTVRGAEKLREELEQLKNVKRPRITVQIAEARKHGDLKENAEYHSAREEQSFCEGRIQEIESKLSNSQIIDITK
ISNDGRVVFGSTVTILNIKNNAVFTYQIVGDDESDFKKNLISINSPMSRGLIGKTVNTIAIIHTPSGNVKYKILKIDYI
>Mature_159_residues
MINLIPMTVRGAEKLREELEQLKNVKRPRITVQIAEARKHGDLKENAEYHSAREEQSFCEGRIQEIESKLSNSQIIDITK
ISNDGRVVFGSTVTILNIKNNAVFTYQIVGDDESDFKKNLISINSPMSRGLIGKTVNTIAIIHTPSGNVKYKILKIDYI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=62.6582278481013, Blast_Score=228, Evalue=2e-61,
Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=34.1935483870968, Blast_Score=96, Evalue=1e-21,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_BUCAP (Q8K9G6)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660712.1
- ProteinModelPortal:   Q8K9G6
- SMR:   Q8K9G6
- EnsemblBacteria:   EBBUCT00000000350
- GeneID:   1005786
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg371
- GeneTree:   EBGT00050000007852
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   BAPH198804:BUSG371-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17957; Mature: 17957

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLIPMTVRGAEKLREELEQLKNVKRPRITVQIAEARKHGDLKENAEYHSAREEQSFCE
CCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GRIQEIESKLSNSQIIDITKISNDGRVVFGSTVTILNIKNNAVFTYQIVGDDESDFKKNL
HHHHHHHHHHCCCEEEEEEEECCCCEEEEECEEEEEEECCCEEEEEEEECCCHHHHHHHH
ISINSPMSRGLIGKTVNTIAIIHTPSGNVKYKILKIDYI
EECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEEECC
>Mature Secondary Structure
MINLIPMTVRGAEKLREELEQLKNVKRPRITVQIAEARKHGDLKENAEYHSAREEQSFCE
CCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GRIQEIESKLSNSQIIDITKISNDGRVVFGSTVTILNIKNNAVFTYQIVGDDESDFKKNL
HHHHHHHHHHCCCEEEEEEEECCCCEEEEECEEEEEEECCCEEEEEEEECCCHHHHHHHH
ISINSPMSRGLIGKTVNTIAIIHTPSGNVKYKILKIDYI
EECCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438