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The map label for this gene is ftsJ

Identifier: 21672644

GI number: 21672644

Start: 424732

End: 425352

Strand: Reverse

Name: ftsJ

Synonym: BUsg370

Alternate gene names: 21672644

Gene position: 425352-424732 (Counterclockwise)

Preceding gene: 21672645

Following gene: 21672643

Centisome position: 66.31

GC content: 27.86

Gene sequence:

>621_bases
ATGATTTCTAAAAAAAAATCAAATAGTTCTAATCGTTGGTTGTCAGAACATTTTCAAGATCAATATGTTAAGGAGGCTAA
AAAGCAAAAAATACGTTCAAGATCTTGGTTTAAATTAGAAGAAATTGATAAAAATAATAGATTGTTTAAACCTGGAATGA
ATGTTCTTGATTTAGGATCTTCTCCTGGGGGTTGGTCTCAATATGCCGCTAAAAAAATAGGGAAAAAAGGATATATTTTA
GCTTGTGATATACTACCTATGAAAAAAATAAAAGGTGTTGATTTTTTTCAAGGAGATTTTTGTAATAAAAAAACATTAAA
TTTGATACTAACCAATTTGTCTAATGTTAATTTAAATTTAATTATGTCTGATATGGCACCTAATATCACAGGTTGTTCAT
CTATTGATATGCCACGGATTATTGAAATATGTAAAACAGTTTTTACAATATCCGATCATGTACTATCTCGAAATGGTGTT
GTATTAGTAAAATCATTTCAAGGAGAAGGTTTTAATGAATTTTTTGCGCAGATGAAAAATTTGTTTTCAAAAATCAAAAT
TTGTAAACCTAAAACTTCTCGATCAAGATCTCGAGAAATATTCATGCTAGCAACCAGATAA

Upstream 100 bases:

>100_bases
ATGTTAAATATAAAATTTTAAAAATAGATTATATTTAAATATATACTTTTTTATAATAAGAAATACAATCTTCTATAATA
TAAAGATATTTGATAAAAAT

Downstream 100 bases:

>100_bases
AAAAATAATAATCTGTGTTGTTCTTTAAAAATAGATGTTAATGAGAGGTTGTTCCCTTGAGTGACATGGTTAAAAACCTG
ATCTTCTGGTTAGTTATTAC

Product: cell division protein FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MISKKKSNSSNRWLSEHFQDQYVKEAKKQKIRSRSWFKLEEIDKNNRLFKPGMNVLDLGSSPGGWSQYAAKKIGKKGYIL
ACDILPMKKIKGVDFFQGDFCNKKTLNLILTNLSNVNLNLIMSDMAPNITGCSSIDMPRIIEICKTVFTISDHVLSRNGV
VLVKSFQGEGFNEFFAQMKNLFSKIKICKPKTSRSRSREIFMLATR

Sequences:

>Translated_206_residues
MISKKKSNSSNRWLSEHFQDQYVKEAKKQKIRSRSWFKLEEIDKNNRLFKPGMNVLDLGSSPGGWSQYAAKKIGKKGYIL
ACDILPMKKIKGVDFFQGDFCNKKTLNLILTNLSNVNLNLIMSDMAPNITGCSSIDMPRIIEICKTVFTISDHVLSRNGV
VLVKSFQGEGFNEFFAQMKNLFSKIKICKPKTSRSRSREIFMLATR
>Mature_206_residues
MISKKKSNSSNRWLSEHFQDQYVKEAKKQKIRSRSWFKLEEIDKNNRLFKPGMNVLDLGSSPGGWSQYAAKKIGKKGYIL
ACDILPMKKIKGVDFFQGDFCNKKTLNLILTNLSNVNLNLIMSDMAPNITGCSSIDMPRIIEICKTVFTISDHVLSRNGV
VLVKSFQGEGFNEFFAQMKNLFSKIKICKPKTSRSRSREIFMLATR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=212, Percent_Identity=33.0188679245283, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI29029591, Length=187, Percent_Identity=32.0855614973262, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI29029589, Length=187, Percent_Identity=32.0855614973262, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI7110661, Length=187, Percent_Identity=32.0855614973262, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI194097365, Length=185, Percent_Identity=26.4864864864865, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1789569, Length=205, Percent_Identity=49.2682926829268, Blast_Score=244, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17554650, Length=188, Percent_Identity=35.6382978723404, Blast_Score=121, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17553474, Length=209, Percent_Identity=31.5789473684211, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71987550, Length=185, Percent_Identity=27.5675675675676, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71987561, Length=185, Percent_Identity=27.5675675675676, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17553860, Length=185, Percent_Identity=27.5675675675676, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319535, Length=195, Percent_Identity=31.2820512820513, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=106, Percent_Identity=45.2830188679245, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319796, Length=185, Percent_Identity=28.1081081081081, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356387, Length=210, Percent_Identity=30.4761904761905, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647580, Length=197, Percent_Identity=28.9340101522843, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648639, Length=194, Percent_Identity=28.8659793814433, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18859957, Length=185, Percent_Identity=26.4864864864865, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_BUCAP (Q8K9G7)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660711.1
- ProteinModelPortal:   Q8K9G7
- SMR:   Q8K9G7
- EnsemblBacteria:   EBBUCT00000000395
- GeneID:   1005787
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg370
- GeneTree:   EBGT00050000008203
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK316048
- BioCyc:   BAPH198804:BUSG370-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23545; Mature: 23545

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: NA

Important sites: ACT_SITE 164-164 BINDING 63-63 BINDING 65-65 BINDING 83-83 BINDING 99-99 BINDING 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISKKKSNSSNRWLSEHFQDQYVKEAKKQKIRSRSWFKLEEIDKNNRLFKPGMNVLDLGS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCEECCCCCEEECCC
SPGGWSQYAAKKIGKKGYILACDILPMKKIKGVDFFQGDFCNKKTLNLILTNLSNVNLNL
CCCCHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCCCCCCCCCHHHHHHEECCCCCEEEE
IMSDMAPNITGCSSIDMPRIIEICKTVFTISDHVLSRNGVVLVKSFQGEGFNEFFAQMKN
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
LFSKIKICKPKTSRSRSREIFMLATR
HHHHHEECCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MISKKKSNSSNRWLSEHFQDQYVKEAKKQKIRSRSWFKLEEIDKNNRLFKPGMNVLDLGS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCEECCCCCEEECCC
SPGGWSQYAAKKIGKKGYILACDILPMKKIKGVDFFQGDFCNKKTLNLILTNLSNVNLNL
CCCCHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCCCCCCCCCHHHHHHEECCCCCEEEE
IMSDMAPNITGCSSIDMPRIIEICKTVFTISDHVLSRNGVVLVKSFQGEGFNEFFAQMKN
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
LFSKIKICKPKTSRSRSREIFMLATR
HHHHHEECCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438