Click here to switch to the map view.

The map label for this gene is pykA

Identifier: 21672587

GI number: 21672587

Start: 355536

End: 356981

Strand: Reverse

Name: pykA

Synonym: BUsg311

Alternate gene names: 21672587

Gene position: 356981-355536 (Counterclockwise)

Preceding gene: 21672592

Following gene: 21672584

Centisome position: 55.65

GC content: 29.94

Gene sequence:

>1446_bases
ATGTTAAATCGTGTTAGAAGAACAAAAATTGTAGCAACTTTAGGACCTTCTACAGATAAAGCAAATAATCTTGAAAAGAT
TATTTTATCTGGAGCAAATGTTCTTCGTTTAAATTTTTCTCATGGTTCATCTGAAGAACATAAAAAAAGAGCGAAAAAAG
CAAAAGAAATAATGTTTAAATTAAATTGTCATATTGCTTTATTAGGTGATTTACAAGGACCAAAAATCAGAATTTCTAAA
TTTAAAAAAAACAGTATTTTTTTAAAAGTTAATGACGTTTTTATATTAGATGCAAATTTAAATGAAAAAAATGGAAACAA
TGAAAGAGTCGGAATTGATTATAAAAAATTACCAAATGACCTAAATGTAAATGATATCTTGTTGTTAGACGACGGAAGAA
TACAATTAAAAGTAATAAAAATTGAAAAATGTGCAATATTTACAAAAGTTATTATAGGTGGCATTCTATCTAATAATAAG
GGTATTAATAAATTAGGTGGTGGTTTATCAGCAGATGCATTAACAGAAAAAGATAAAAAAGATATAATTCTTGCTTCTGA
AATTGATGTAGATTATTTGGCCGTATCGTTCCCTCGTTGTGCTGATGATTTAATAAAAGCCAGAGAGTTATTAAAAAAAT
CTGGTAGTAACGCTCAAATTATAGCTAAAATAGAAAGAGCCGAAGCTGTATTTGATCAAAATGCTATAGAAAATATAATA
TTGTCTTCAGATGCAATTATGATTGCTAGAGGAGACTTAGGTGTCGAAATTGGTGATGCAGAATTGGTAGGTATTCAAAA
AAAATTAATTAGAACTGCTAGAAAATTAAATAAGGTAGCAATTACTGCAACACAAATGATGGAATCAATGATTTTAAATC
CGTTACCTACTCGTGCAGAAGTTATGGATGTAGCCAATGCTGTGTTAGATGGAAGCGATGCAGTCATGCTGTCTGCGGAA
ACTGCATCTGGAAAATATCCATCTGAAACTGTGATAAGTATGGCTAAAGTTTGCAAAGGGGCAGAAAAAGTTCCTAGTAT
TAATGTTTCTAGACATCGTCTTAATGTAGAATTTCAAAGCATTGAGGAAGCCATTGCTATGTCATCAATGTATGCGGCGA
ATCACTTAAAAGGAATTACAGCAATTATTACTATGACAGAATCTGGTAAAACAGCATTAATGACTTCTAGAATTACTTCT
GGACTACCGATTTTTGCTTTATCAAAACATAAAAAAACTTTAAATTTAACTGCTCTCTATAGAGGTGTGATTCCTATTTA
TTTTGATAGTGATAAGGATGGTGTTGAAGCTGCTAATGAAGCAATAATTCTTTTGTGCAAGAAAAAATTTCTATTTAATA
ATGATTTAGTCATTGTCACTCAAGGTGACATAATGGGGAAAATAGGTAAAACGAATACCAGTAGAATTTTAAGAGTTACA
TATTAA

Upstream 100 bases:

>100_bases
TTTTATTAAAAACTGATTATAAAATTTCTTACTCTTTTATATTTCGATTAAATTTTTAAAATTTTAAAATACATATAATT
AATAAAATTAAGAGGTTTTT

Downstream 100 bases:

>100_bases
AAATTTCTATTAGAAAAACTTATTTTAATTTTGATAATATGTTTTTAGACTCTTTTAAATATTTGATTCTTTCACTTTTT
GTTAGCTGTGTCGACAAAAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MLNRVRRTKIVATLGPSTDKANNLEKIILSGANVLRLNFSHGSSEEHKKRAKKAKEIMFKLNCHIALLGDLQGPKIRISK
FKKNSIFLKVNDVFILDANLNEKNGNNERVGIDYKKLPNDLNVNDILLLDDGRIQLKVIKIEKCAIFTKVIIGGILSNNK
GINKLGGGLSADALTEKDKKDIILASEIDVDYLAVSFPRCADDLIKARELLKKSGSNAQIIAKIERAEAVFDQNAIENII
LSSDAIMIARGDLGVEIGDAELVGIQKKLIRTARKLNKVAITATQMMESMILNPLPTRAEVMDVANAVLDGSDAVMLSAE
TASGKYPSETVISMAKVCKGAEKVPSINVSRHRLNVEFQSIEEAIAMSSMYAANHLKGITAIITMTESGKTALMTSRITS
GLPIFALSKHKKTLNLTALYRGVIPIYFDSDKDGVEAANEAIILLCKKKFLFNNDLVIVTQGDIMGKIGKTNTSRILRVT
Y

Sequences:

>Translated_481_residues
MLNRVRRTKIVATLGPSTDKANNLEKIILSGANVLRLNFSHGSSEEHKKRAKKAKEIMFKLNCHIALLGDLQGPKIRISK
FKKNSIFLKVNDVFILDANLNEKNGNNERVGIDYKKLPNDLNVNDILLLDDGRIQLKVIKIEKCAIFTKVIIGGILSNNK
GINKLGGGLSADALTEKDKKDIILASEIDVDYLAVSFPRCADDLIKARELLKKSGSNAQIIAKIERAEAVFDQNAIENII
LSSDAIMIARGDLGVEIGDAELVGIQKKLIRTARKLNKVAITATQMMESMILNPLPTRAEVMDVANAVLDGSDAVMLSAE
TASGKYPSETVISMAKVCKGAEKVPSINVSRHRLNVEFQSIEEAIAMSSMYAANHLKGITAIITMTESGKTALMTSRITS
GLPIFALSKHKKTLNLTALYRGVIPIYFDSDKDGVEAANEAIILLCKKKFLFNNDLVIVTQGDIMGKIGKTNTSRILRVT
Y
>Mature_481_residues
MLNRVRRTKIVATLGPSTDKANNLEKIILSGANVLRLNFSHGSSEEHKKRAKKAKEIMFKLNCHIALLGDLQGPKIRISK
FKKNSIFLKVNDVFILDANLNEKNGNNERVGIDYKKLPNDLNVNDILLLDDGRIQLKVIKIEKCAIFTKVIIGGILSNNK
GINKLGGGLSADALTEKDKKDIILASEIDVDYLAVSFPRCADDLIKARELLKKSGSNAQIIAKIERAEAVFDQNAIENII
LSSDAIMIARGDLGVEIGDAELVGIQKKLIRTARKLNKVAITATQMMESMILNPLPTRAEVMDVANAVLDGSDAVMLSAE
TASGKYPSETVISMAKVCKGAEKVPSINVSRHRLNVEFQSIEEAIAMSSMYAANHLKGITAIITMTESGKTALMTSRITS
GLPIFALSKHKKTLNLTALYRGVIPIYFDSDKDGVEAANEAIILLCKKKFLFNNDLVIVTQGDIMGKIGKTNTSRILRVT
Y

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family

Homologues:

Organism=Homo sapiens, GI33286422, Length=511, Percent_Identity=35.812133072407, Blast_Score=245, Evalue=8e-65,
Organism=Homo sapiens, GI33286420, Length=511, Percent_Identity=35.812133072407, Blast_Score=245, Evalue=8e-65,
Organism=Homo sapiens, GI33286418, Length=511, Percent_Identity=35.6164383561644, Blast_Score=239, Evalue=5e-63,
Organism=Homo sapiens, GI10835121, Length=510, Percent_Identity=33.1372549019608, Blast_Score=229, Evalue=5e-60,
Organism=Homo sapiens, GI32967597, Length=510, Percent_Identity=33.1372549019608, Blast_Score=229, Evalue=5e-60,
Organism=Homo sapiens, GI310128732, Length=297, Percent_Identity=34.6801346801347, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI310128730, Length=297, Percent_Identity=34.6801346801347, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI310128736, Length=246, Percent_Identity=33.739837398374, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI310128734, Length=246, Percent_Identity=33.739837398374, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI310128738, Length=222, Percent_Identity=33.7837837837838, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788160, Length=479, Percent_Identity=69.5198329853862, Blast_Score=704, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=477, Percent_Identity=38.7840670859539, Blast_Score=290, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=492, Percent_Identity=34.7560975609756, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI71984406, Length=498, Percent_Identity=36.144578313253, Blast_Score=235, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17506829, Length=498, Percent_Identity=36.144578313253, Blast_Score=235, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI71984413, Length=498, Percent_Identity=36.144578313253, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17506831, Length=498, Percent_Identity=36.144578313253, Blast_Score=234, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6319279, Length=448, Percent_Identity=35.0446428571429, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6324923, Length=495, Percent_Identity=31.7171717171717, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=36.4372469635627, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=36.4372469635627, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24648966, Length=421, Percent_Identity=34.4418052256532, Blast_Score=215, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24581235, Length=433, Percent_Identity=31.8706697459584, Blast_Score=214, Evalue=7e-56,
Organism=Drosophila melanogaster, GI24646914, Length=281, Percent_Identity=34.5195729537367, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KPYK_BUCAP (Q8K9M3)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660654.1
- ProteinModelPortal:   Q8K9M3
- SMR:   Q8K9M3
- EnsemblBacteria:   EBBUCT00000000272
- GeneID:   1005515
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg311
- GeneTree:   EBGT00050000007890
- HOGENOM:   HBG734376
- OMA:   SRSRQLN
- ProtClustDB:   CLSK315882
- BioCyc:   BAPH198804:BUSG311-MONOMER
- GO:   GO:0006096
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806
- Gene3D:   G3DSA:2.40.33.10
- Gene3D:   G3DSA:3.20.20.60
- Gene3D:   G3DSA:3.40.1380.20
- PANTHER:   PTHR11817
- PRINTS:   PR01050
- TIGRFAMs:   TIGR01064

Pfam domain/function: PF00224 PK; PF02887 PK_C; SSF50800 PK_B_barrel_like; SSF52935 Pyruvate_kinase; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.1.40

Molecular weight: Translated: 52632; Mature: 52632

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: BINDING 36-36 BINDING 251-251 BINDING 252-252 BINDING 284-284

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNRVRRTKIVATLGPSTDKANNLEKIILSGANVLRLNFSHGSSEEHKKRAKKAKEIMFK
CCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHEEEE
LNCHIALLGDLQGPKIRISKFKKNSIFLKVNDVFILDANLNEKNGNNERVGIDYKKLPND
EEEEEEEEECCCCCEEEEEEECCCEEEEEEEEEEEEECCCCCCCCCCCEEECCHHHCCCC
LNVNDILLLDDGRIQLKVIKIEKCAIFTKVIIGGILSNNKGINKLGGGLSADALTEKDKK
CCCCEEEEEECCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHCCCCCC
DIILASEIDVDYLAVSFPRCADDLIKARELLKKSGSNAQIIAKIERAEAVFDQNAIENII
CEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHE
LSSDAIMIARGDLGVEIGDAELVGIQKKLIRTARKLNKVAITATQMMESMILNPLPTRAE
ECCCEEEEEECCCCEEECCCCEEHHHHHHHHHHHHHHHHEEEHHHHHHHHHCCCCCCHHH
VMDVANAVLDGSDAVMLSAETASGKYPSETVISMAKVCKGAEKVPSINVSRHRLNVEFQS
HHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHHCCCCCCEEEEEEEEHHH
IEEAIAMSSMYAANHLKGITAIITMTESGKTALMTSRITSGLPIFALSKHKKTLNLTALY
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEHHHCCCCCEEEEECCCCEEEEEEEE
RGVIPIYFDSDKDGVEAANEAIILLCKKKFLFNNDLVIVTQGDIMGKIGKTNTSRILRVT
CCCEEEEECCCCCCCHHCCCEEEEEEEHHHEECCCEEEEECCCEEECCCCCCCCEEEEEE
Y
C
>Mature Secondary Structure
MLNRVRRTKIVATLGPSTDKANNLEKIILSGANVLRLNFSHGSSEEHKKRAKKAKEIMFK
CCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHEEEE
LNCHIALLGDLQGPKIRISKFKKNSIFLKVNDVFILDANLNEKNGNNERVGIDYKKLPND
EEEEEEEEECCCCCEEEEEEECCCEEEEEEEEEEEEECCCCCCCCCCCEEECCHHHCCCC
LNVNDILLLDDGRIQLKVIKIEKCAIFTKVIIGGILSNNKGINKLGGGLSADALTEKDKK
CCCCEEEEEECCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHCCCCCC
DIILASEIDVDYLAVSFPRCADDLIKARELLKKSGSNAQIIAKIERAEAVFDQNAIENII
CEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHE
LSSDAIMIARGDLGVEIGDAELVGIQKKLIRTARKLNKVAITATQMMESMILNPLPTRAE
ECCCEEEEEECCCCEEECCCCEEHHHHHHHHHHHHHHHHEEEHHHHHHHHHCCCCCCHHH
VMDVANAVLDGSDAVMLSAETASGKYPSETVISMAKVCKGAEKVPSINVSRHRLNVEFQS
HHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHHCCCCCCEEEEEEEEHHH
IEEAIAMSSMYAANHLKGITAIITMTESGKTALMTSRITSGLPIFALSKHKKTLNLTALY
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEHHHCCCCCEEEEECCCCEEEEEEEE
RGVIPIYFDSDKDGVEAANEAIILLCKKKFLFNNDLVIVTQGDIMGKIGKTNTSRILRVT
CCCEEEEECCCCCCCHHCCCEEEEEEEHHHEECCCEEEEECCCEEECCCCCCCCEEEEEE
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438