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The map label for this gene is aroA

Identifier: 21672577

GI number: 21672577

Start: 344439

End: 345725

Strand: Reverse

Name: aroA

Synonym: BUsg301

Alternate gene names: 21672577

Gene position: 345725-344439 (Counterclockwise)

Preceding gene: 21672578

Following gene: 21672576

Centisome position: 53.9

GC content: 29.99

Gene sequence:

>1287_bases
ATGCAAAAATTTTTGGAGTTAAAACCAGTTTCTTATATTAATGGAACGATTTATTTACCTGGTTCAAAAAGTATATCGAA
CAGAGTTTTACTGTTATCTGCTATGGCTAATGGTATTACTTGTTTGACAAATTTACTAGATAGTCAAGATACTCAATATA
TGTTAAATGCTTTAAGAAAAATTGGGATCAAATTTTTTTTATCAAATAATAATACTACTTGTCATGTCCATGGTATTGGA
AAAGCTTTTCATTTATCGCATCCTATTTCATTATTTTTAGGCAATGCGGGAACAGCTATGCGACCACTTCTTGCTGCTTT
ATCGTTATATGAAAACAATGTTGTCTTAAGTGGAGATGACAGAATGCATGAAAGACCGATTGCACATCTTGTTGATGCTC
TCAAACAAGGTGGAGCAACTCTTGAATATAAAAAAGGTATAGGATATCCTCCTGTTTTGACGAAAGGTGGGTTTAAAGGT
GGTTCTATTATGCTAGATGGAAGTATTTCTAGTCAATTTTTAACATCTTTATTAATGGTTGCACCACTAGCATTACAAAA
TACTAATATATTTATTAAAGGAAATTTGGTTTCTAAACCTTACATTGATATCACATTAAATCTCATGAAATCTTTTGGTG
TCAATATTGTAAATGATTGTTATAAATCTTTTTATATAAAAGGGAATCAAAAATATGAATCACCAGGAAATTACTTAGTT
GAAGGCGATGCATCTTCAGCATCATATTTTTTAGCTGCTGCTGCTATTAAAGGTGGTTCGGTAAAAGTTGTTGGAGTTGG
TAAAAAAAGCGTTCAGGGTGATATAAAATTTGCTGATGTACTTGAAAAAATGGGAGCTATTATTGATTGGGGTGATTCTT
TCATTGTTTGCAGGCATAATAAATTAGAAAAAATAGATTTAGATATGAATCATATCCCTGATGCAGCAATGACTATTGCA
ATAGTAGCTCTTTTTGCAAAAGGAACTTCAATTATTAAAAATATATATAACTGGCGTGTTAAAGAAACTGATCGATTATC
TGCAATGAGTAAAGAATTAAAAAAAGTTGGTGCAATAATTAAAGAAGGAAGAGATTGTTTATCTATTACTCCTCCTAATT
TTTTCAAATTTGCAGAAATTGATACATATAATGATCATCGTATGGCTATGTGTTTTTCTCTCATATGTTTATCTGGAATA
AGTGTTAGGATACTTAATCCAAATTGTATTTCTAAAACTTTTCCATCTTATTTTGAAAATTTTTTAAAGATTAGCAGATT
TGATTGA

Upstream 100 bases:

>100_bases
AAATTTATGTTATATTTTGAAAAAAAGTATGGATAAAACATTTTTTTATTTTTGAAGAAATAAATTAAAAATTTTATTTT
AATTAGAGCGTATTTTTATT

Downstream 100 bases:

>100_bases
TTTTTAACATTATAGATAGTTTTATATGTTTAAATTTAATTTTTTTGTACTTTTATAAAAATGAAAAATAAAACTACCTT
TTTATCATTAAAGGTTCTGG

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MQKFLELKPVSYINGTIYLPGSKSISNRVLLLSAMANGITCLTNLLDSQDTQYMLNALRKIGIKFFLSNNNTTCHVHGIG
KAFHLSHPISLFLGNAGTAMRPLLAALSLYENNVVLSGDDRMHERPIAHLVDALKQGGATLEYKKGIGYPPVLTKGGFKG
GSIMLDGSISSQFLTSLLMVAPLALQNTNIFIKGNLVSKPYIDITLNLMKSFGVNIVNDCYKSFYIKGNQKYESPGNYLV
EGDASSASYFLAAAAIKGGSVKVVGVGKKSVQGDIKFADVLEKMGAIIDWGDSFIVCRHNKLEKIDLDMNHIPDAAMTIA
IVALFAKGTSIIKNIYNWRVKETDRLSAMSKELKKVGAIIKEGRDCLSITPPNFFKFAEIDTYNDHRMAMCFSLICLSGI
SVRILNPNCISKTFPSYFENFLKISRFD

Sequences:

>Translated_428_residues
MQKFLELKPVSYINGTIYLPGSKSISNRVLLLSAMANGITCLTNLLDSQDTQYMLNALRKIGIKFFLSNNNTTCHVHGIG
KAFHLSHPISLFLGNAGTAMRPLLAALSLYENNVVLSGDDRMHERPIAHLVDALKQGGATLEYKKGIGYPPVLTKGGFKG
GSIMLDGSISSQFLTSLLMVAPLALQNTNIFIKGNLVSKPYIDITLNLMKSFGVNIVNDCYKSFYIKGNQKYESPGNYLV
EGDASSASYFLAAAAIKGGSVKVVGVGKKSVQGDIKFADVLEKMGAIIDWGDSFIVCRHNKLEKIDLDMNHIPDAAMTIA
IVALFAKGTSIIKNIYNWRVKETDRLSAMSKELKKVGAIIKEGRDCLSITPPNFFKFAEIDTYNDHRMAMCFSLICLSGI
SVRILNPNCISKTFPSYFENFLKISRFD
>Mature_428_residues
MQKFLELKPVSYINGTIYLPGSKSISNRVLLLSAMANGITCLTNLLDSQDTQYMLNALRKIGIKFFLSNNNTTCHVHGIG
KAFHLSHPISLFLGNAGTAMRPLLAALSLYENNVVLSGDDRMHERPIAHLVDALKQGGATLEYKKGIGYPPVLTKGGFKG
GSIMLDGSISSQFLTSLLMVAPLALQNTNIFIKGNLVSKPYIDITLNLMKSFGVNIVNDCYKSFYIKGNQKYESPGNYLV
EGDASSASYFLAAAAIKGGSVKVVGVGKKSVQGDIKFADVLEKMGAIIDWGDSFIVCRHNKLEKIDLDMNHIPDAAMTIA
IVALFAKGTSIIKNIYNWRVKETDRLSAMSKELKKVGAIIKEGRDCLSITPPNFFKFAEIDTYNDHRMAMCFSLICLSGI
SVRILNPNCISKTFPSYFENFLKISRFD

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=422, Percent_Identity=63.9810426540284, Blast_Score=559, Evalue=1e-160,
Organism=Saccharomyces cerevisiae, GI6320332, Length=446, Percent_Identity=37.219730941704, Blast_Score=237, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_BUCAP (Q59178)

Other databases:

- EMBL:   L43549
- EMBL:   AE013218
- RefSeq:   NP_660644.1
- ProteinModelPortal:   Q59178
- SMR:   Q59178
- EnsemblBacteria:   EBBUCT00000000096
- GeneID:   1005505
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg301
- GeneTree:   EBGT00050000008084
- HOGENOM:   HBG646626
- OMA:   GADIEWG
- ProtClustDB:   PRK02427
- BioCyc:   BAPH198804:BUSG301-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 47030; Mature: 47030

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKFLELKPVSYINGTIYLPGSKSISNRVLLLSAMANGITCLTNLLDSQDTQYMLNALRK
CCCCCCCCCCEEECCEEEECCCCCCCCCEEEEEHHHCHHHHHHHHHCCCCHHHHHHHHHH
IGIKFFLSNNNTTCHVHGIGKAFHLSHPISLFLGNAGTAMRPLLAALSLYENNVVLSGDD
CCEEEEEECCCCEEEEEECCCEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCEEECCCC
RMHERPIAHLVDALKQGGATLEYKKGIGYPPVLTKGGFKGGSIMLDGSISSQFLTSLLMV
CHHCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHH
APLALQNTNIFIKGNLVSKPYIDITLNLMKSFGVNIVNDCYKSFYIKGNQKYESPGNYLV
HHHEECCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHEEECCCCCCCCCCCEEE
EGDASSASYFLAAAAIKGGSVKVVGVGKKSVQGDIKFADVLEKMGAIIDWGDSFIVCRHN
ECCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEECCCCEEEEECC
KLEKIDLDMNHIPDAAMTIAIVALFAKGTSIIKNIYNWRVKETDRLSAMSKELKKVGAII
CCEEEECCHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KEGRDCLSITPPNFFKFAEIDTYNDHRMAMCFSLICLSGISVRILNPNCISKTFPSYFEN
HCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
FLKISRFD
HHHHCCCC
>Mature Secondary Structure
MQKFLELKPVSYINGTIYLPGSKSISNRVLLLSAMANGITCLTNLLDSQDTQYMLNALRK
CCCCCCCCCCEEECCEEEECCCCCCCCCEEEEEHHHCHHHHHHHHHCCCCHHHHHHHHHH
IGIKFFLSNNNTTCHVHGIGKAFHLSHPISLFLGNAGTAMRPLLAALSLYENNVVLSGDD
CCEEEEEECCCCEEEEEECCCEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCEEECCCC
RMHERPIAHLVDALKQGGATLEYKKGIGYPPVLTKGGFKGGSIMLDGSISSQFLTSLLMV
CHHCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHH
APLALQNTNIFIKGNLVSKPYIDITLNLMKSFGVNIVNDCYKSFYIKGNQKYESPGNYLV
HHHEECCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHEEECCCCCCCCCCCEEE
EGDASSASYFLAAAAIKGGSVKVVGVGKKSVQGDIKFADVLEKMGAIIDWGDSFIVCRHN
ECCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEECCCCEEEEECC
KLEKIDLDMNHIPDAAMTIAIVALFAKGTSIIKNIYNWRVKETDRLSAMSKELKKVGAII
CCEEEECCHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KEGRDCLSITPPNFFKFAEIDTYNDHRMAMCFSLICLSGISVRILNPNCISKTFPSYFEN
HCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
FLKISRFD
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8574133; 12089438