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The map label for this gene is tpiA

Identifier: 21672574

GI number: 21672574

Start: 340759

End: 341523

Strand: Reverse

Name: tpiA

Synonym: BUsg297

Alternate gene names: 21672574

Gene position: 341523-340759 (Counterclockwise)

Preceding gene: 21672575

Following gene: 21672567

Centisome position: 53.24

GC content: 24.05

Gene sequence:

>765_bases
ATGAAAAAAAAAATTATTGCAGCTAATTGGAAATTAAATGGTAGTATAAAAACTATTTCTTATTTTTTAACTTTTTTAAA
ATCACAAATTTCATCTTTTTTAAAAAACAATATTATTATAATTGCACCTCCGACTGTATTTTTAGAAAGAGTATACAAAG
ATATAAATAGTATAAATATTCATCTTGCTGCACAAAATATAGATGTAAATCTAACAGGTGCATTTACTGGTGAGAACTCT
GCTTTAATGATGAAAGATATTGGTGTTAAATATATTATTATTGGTCATTCTGAAAGACGTTTACTTCATAATGAAAACAA
TGAAATCATTGCGAAAAAATTTTGTTTAGTAAAAAATTTGAATTTAGTGCCTATTTTATGTATAGGTGAAACTGAAGCAG
AAAAAAAATCTAACAAAACTGAAAAAATTTTAAAAGAACAATTAAATTCTATATTTAATTCTTTCGGAGAAAAAGCATTT
AGAAATGCAGTTATTGCATATGAACCTATTTGGTCTATTGGAACAGGTGTTTCAGCGGATCCTAAAAATGTACAACTGAT
ACATAAATTTATAAAAAATTATATAAAAAAATATGACATTGTCAGTGCAGAAAATTTGATTGTACAATATGGAGGTTCTG
TTACTTCATTAAATGCAGGAAATTTTCTTAAACAACCTGATATTGATGGATTATTAATCGGTAGTGCATCTTTAAAACAT
GAAGAATTTTTAAAAATTATAAAAATATCAGATAGTTTTTTATAA

Upstream 100 bases:

>100_bases
TAAATAGTATATTTTAATACTTGTTGAATAGAAACTAAGTTGATATAATTTTTTATAAAAAACTTAGTTTCTATAACGTT
AAAAAATTGAGTATATTAAT

Downstream 100 bases:

>100_bases
AAAACTATTTTTTTATATAAAGGATATATATCCATCCACCTAAATTGATACCGATAATAGGAGCAATAGTGGGTATTAAA
AAATAAGGAAAAATGATATT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKKKIIAANWKLNGSIKTISYFLTFLKSQISSFLKNNIIIIAPPTVFLERVYKDINSINIHLAAQNIDVNLTGAFTGENS
ALMMKDIGVKYIIIGHSERRLLHNENNEIIAKKFCLVKNLNLVPILCIGETEAEKKSNKTEKILKEQLNSIFNSFGEKAF
RNAVIAYEPIWSIGTGVSADPKNVQLIHKFIKNYIKKYDIVSAENLIVQYGGSVTSLNAGNFLKQPDIDGLLIGSASLKH
EEFLKIIKISDSFL

Sequences:

>Translated_254_residues
MKKKIIAANWKLNGSIKTISYFLTFLKSQISSFLKNNIIIIAPPTVFLERVYKDINSINIHLAAQNIDVNLTGAFTGENS
ALMMKDIGVKYIIIGHSERRLLHNENNEIIAKKFCLVKNLNLVPILCIGETEAEKKSNKTEKILKEQLNSIFNSFGEKAF
RNAVIAYEPIWSIGTGVSADPKNVQLIHKFIKNYIKKYDIVSAENLIVQYGGSVTSLNAGNFLKQPDIDGLLIGSASLKH
EEFLKIIKISDSFL
>Mature_254_residues
MKKKIIAANWKLNGSIKTISYFLTFLKSQISSFLKNNIIIIAPPTVFLERVYKDINSINIHLAAQNIDVNLTGAFTGENS
ALMMKDIGVKYIIIGHSERRLLHNENNEIIAKKFCLVKNLNLVPILCIGETEAEKKSNKTEKILKEQLNSIFNSFGEKAF
RNAVIAYEPIWSIGTGVSADPKNVQLIHKFIKNYIKKYDIVSAENLIVQYGGSVTSLNAGNFLKQPDIDGLLIGSASLKH
EEFLKIIKISDSFL

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=248, Percent_Identity=36.2903225806452, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI4507645, Length=248, Percent_Identity=36.2903225806452, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI1790353, Length=252, Percent_Identity=47.6190476190476, Blast_Score=271, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=36.8421052631579, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6320255, Length=245, Percent_Identity=40, Blast_Score=176, Evalue=3e-45,
Organism=Drosophila melanogaster, GI28572008, Length=251, Percent_Identity=39.4422310756972, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI28572006, Length=251, Percent_Identity=39.4422310756972, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI28572004, Length=251, Percent_Identity=39.4422310756972, Blast_Score=176, Evalue=1e-44,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_BUCAP (Q59179)

Other databases:

- EMBL:   L43549
- EMBL:   AE013218
- RefSeq:   NP_660641.1
- ProteinModelPortal:   Q59179
- SMR:   Q59179
- EnsemblBacteria:   EBBUCT00000000218
- GeneID:   1005501
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg297
- GeneTree:   EBGT00050000007988
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   BAPH198804:BUSG297-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 28459; Mature: 28459

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 168-168 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIIAANWKLNGSIKTISYFLTFLKSQISSFLKNNIIIIAPPTVFLERVYKDINSINI
CCCCEEEECEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHCCEEE
HLAAQNIDVNLTGAFTGENSALMMKDIGVKYIIIGHSERRLLHNENNEIIAKKFCLVKNL
EEEEEEEEEEEEEEEECCCCEEEEEECCEEEEEEECCCHHHHHCCCCCCHHHHHHHHCCC
NLVPILCIGETEAEKKSNKTEKILKEQLNSIFNSFGEKAFRNAVIAYEPIWSIGTGVSAD
CEEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHCCCCCCCC
PKNVQLIHKFIKNYIKKYDIVSAENLIVQYGGSVTSLNAGNFLKQPDIDGLLIGSASLKH
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCCCCCEEEECCCCCCH
EEFLKIIKISDSFL
HHHHHHHEECCCCC
>Mature Secondary Structure
MKKKIIAANWKLNGSIKTISYFLTFLKSQISSFLKNNIIIIAPPTVFLERVYKDINSINI
CCCCEEEECEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHCCEEE
HLAAQNIDVNLTGAFTGENSALMMKDIGVKYIIIGHSERRLLHNENNEIIAKKFCLVKNL
EEEEEEEEEEEEEEEECCCCEEEEEECCEEEEEEECCCHHHHHCCCCCCHHHHHHHHCCC
NLVPILCIGETEAEKKSNKTEKILKEQLNSIFNSFGEKAFRNAVIAYEPIWSIGTGVSAD
CEEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHCCCCCCCC
PKNVQLIHKFIKNYIKKYDIVSAENLIVQYGGSVTSLNAGNFLKQPDIDGLLIGSASLKH
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCCCCCEEEECCCCCCH
EEFLKIIKISDSFL
HHHHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438