Click here to switch to the map view.

The map label for this gene is lepB

Identifier: 21672531

GI number: 21672531

Start: 284909

End: 285847

Strand: Reverse

Name: lepB

Synonym: BUsg250

Alternate gene names: 21672531

Gene position: 285847-284909 (Counterclockwise)

Preceding gene: 21672532

Following gene: 21672530

Centisome position: 44.56

GC content: 22.47

Gene sequence:

>939_bases
ATGCGTAATATATTGACTATTTTTTTATTAACAAGCACTTTTTTTACAGGTATATTATGGATCATTGATCATATTCTATT
AATTAAAAATTACTTTTATAATAAAAAAAAAACAAAAAATAATAATACAATTTTAATAAATAAAGTGATTTTAGAAAATA
AAAAATGTTTTTTTCGATCTTTATCATCTCTATTTCCAACTTTTTTTATTGTTTTCATAATACGTTCCTTTATTTATGAA
CCTTTTCAAATTCCTTCAGGTTCAATGATGCCGACTCTTTTGATAGGAGATTTTATTTTAGTAAAAAAATTTTCATACGG
TATTAAGGAACCTATTACTAATAAAACTATAATTAAAATGAATCTTCCTCAACGTGGTGATATTGTAGTTTTTAAACATC
CTAAAAATAATATTGATTATATAAAACGTGTAGTAGGATTACCTGGAGATAAAATTCAATATGATATAAATAGAAAAAAA
ATAAAAATTTGTATTAATTATACTAATCAAAAAAACTGTGAAAACAAGTTGTTTATTACATATTCTAAACCTAAATTAAG
CAACTTTTTTCAGAAAATATATCTTTTGAAATCAAGAACAAATGAAGAAGAGAAAGTATACAATTCTATTTATTTTAAGA
AAGTTGAAGAAAAAATTAATAATTTAAAACATAATATACTGATATTAGACGGAATTAACAGTAAAATAAACGATTATTAT
CAGCAAAAGGGTATGCCTAAATTAATATGGATAGTACCTAAGAATAAATATTTTATGATGGGGGATAATCGTGATAATAG
TTTAGATAGTCGTTATTGGGGTTTTGTACCTGAAGAGAATTTATTAGGAAAAGCTACAAAAATATGGATGAGTTTTGAAA
AAAAAGAAAATGAATGGCCTACAGGTATACGTATTAAAAGAATTGGAAACATATATTAA

Upstream 100 bases:

>100_bases
GGTAAGAAAAGAATGAAAAAAATAGGAAACGTCAATATGCCAAAAACAGCTTTTCTTGACATTTTAAATATCCATAAGGA
TTAAAACATAAGGAGAAATA

Downstream 100 bases:

>100_bases
AAATAATAATTAATATAGCAGAATATAATCAGTATTATATATTAATATTTGAAATATATCTTCAATGTTATCAAAATTTT
ACTGTTTTTCCTGTTATATG

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MRNILTIFLLTSTFFTGILWIIDHILLIKNYFYNKKKTKNNNTILINKVILENKKCFFRSLSSLFPTFFIVFIIRSFIYE
PFQIPSGSMMPTLLIGDFILVKKFSYGIKEPITNKTIIKMNLPQRGDIVVFKHPKNNIDYIKRVVGLPGDKIQYDINRKK
IKICINYTNQKNCENKLFITYSKPKLSNFFQKIYLLKSRTNEEEKVYNSIYFKKVEEKINNLKHNILILDGINSKINDYY
QQKGMPKLIWIVPKNKYFMMGDNRDNSLDSRYWGFVPEENLLGKATKIWMSFEKKENEWPTGIRIKRIGNIY

Sequences:

>Translated_312_residues
MRNILTIFLLTSTFFTGILWIIDHILLIKNYFYNKKKTKNNNTILINKVILENKKCFFRSLSSLFPTFFIVFIIRSFIYE
PFQIPSGSMMPTLLIGDFILVKKFSYGIKEPITNKTIIKMNLPQRGDIVVFKHPKNNIDYIKRVVGLPGDKIQYDINRKK
IKICINYTNQKNCENKLFITYSKPKLSNFFQKIYLLKSRTNEEEKVYNSIYFKKVEEKINNLKHNILILDGINSKINDYY
QQKGMPKLIWIVPKNKYFMMGDNRDNSLDSRYWGFVPEENLLGKATKIWMSFEKKENEWPTGIRIKRIGNIY
>Mature_312_residues
MRNILTIFLLTSTFFTGILWIIDHILLIKNYFYNKKKTKNNNTILINKVILENKKCFFRSLSSLFPTFFIVFIIRSFIYE
PFQIPSGSMMPTLLIGDFILVKKFSYGIKEPITNKTIIKMNLPQRGDIVVFKHPKNNIDYIKRVVGLPGDKIQYDINRKK
IKICINYTNQKNCENKLFITYSKPKLSNFFQKIYLLKSRTNEEEKVYNSIYFKKVEEKINNLKHNILILDGINSKINDYY
QQKGMPKLIWIVPKNKYFMMGDNRDNSLDSRYWGFVPEENLLGKATKIWMSFEKKENEWPTGIRIKRIGNIY

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family

Homologues:

Organism=Escherichia coli, GI1788921, Length=333, Percent_Identity=47.4474474474475, Blast_Score=309, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEP_BUCAP (Q8K9R0)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660598.1
- ProteinModelPortal:   Q8K9R0
- SMR:   Q8K9R0
- MEROPS:   S26.001
- EnsemblBacteria:   EBBUCT00000000254
- GeneID:   1005452
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg250
- GeneTree:   EBGT00050000007850
- HOGENOM:   HBG596607
- OMA:   VAVFKYP
- ProtClustDB:   CLSK315834
- BioCyc:   BAPH198804:BUSG250-MONOMER
- GO:   GO:0006508
- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- InterPro:   IPR019766
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:2.170.230.10
- PRINTS:   PR00727
- TIGRFAMs:   TIGR02227

Pfam domain/function: PF00717 Peptidase_S24; PF10502 Peptidase_S26; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.89

Molecular weight: Translated: 37103; Mature: 37103

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS00501 SPASE_I_1; PS00760 SPASE_I_2; PS00761 SPASE_I_3

Important sites: ACT_SITE 88-88 ACT_SITE 142-142

Signals:

None

Transmembrane regions:

HASH(0x4048bfc4)-; HASH(0xc3f7c90)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNILTIFLLTSTFFTGILWIIDHILLIKNYFYNKKKTKNNNTILINKVILENKKCFFRS
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHH
LSSLFPTFFIVFIIRSFIYEPFQIPSGSMMPTLLIGDFILVKKFSYGIKEPITNKTIIKM
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
NLPQRGDIVVFKHPKNNIDYIKRVVGLPGDKIQYDINRKKIKICINYTNQKNCENKLFIT
ECCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEECCCEEEEEEEEECCCCCCCCCEEEE
YSKPKLSNFFQKIYLLKSRTNEEEKVYNSIYFKKVEEKINNLKHNILILDGINSKINDYY
ECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
QQKGMPKLIWIVPKNKYFMMGDNRDNSLDSRYWGFVPEENLLGKATKIWMSFEKKENEWP
HHCCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHEEEECCCCCCCC
TGIRIKRIGNIY
CCEEEEECCCCH
>Mature Secondary Structure
MRNILTIFLLTSTFFTGILWIIDHILLIKNYFYNKKKTKNNNTILINKVILENKKCFFRS
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHH
LSSLFPTFFIVFIIRSFIYEPFQIPSGSMMPTLLIGDFILVKKFSYGIKEPITNKTIIKM
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
NLPQRGDIVVFKHPKNNIDYIKRVVGLPGDKIQYDINRKKIKICINYTNQKNCENKLFIT
ECCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEECCCEEEEEEEEECCCCCCCCCEEEE
YSKPKLSNFFQKIYLLKSRTNEEEKVYNSIYFKKVEEKINNLKHNILILDGINSKINDYY
ECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
QQKGMPKLIWIVPKNKYFMMGDNRDNSLDSRYWGFVPEENLLGKATKIWMSFEKKENEWP
HHCCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHEEEECCCCCCCC
TGIRIKRIGNIY
CCEEEEECCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12089438