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The map label for this gene is ftsI

Identifier: 21672498

GI number: 21672498

Start: 246172

End: 247878

Strand: Reverse

Name: ftsI

Synonym: BUsg216

Alternate gene names: 21672498

Gene position: 247878-246172 (Counterclockwise)

Preceding gene: 21672499

Following gene: 21672497

Centisome position: 38.64

GC content: 26.77

Gene sequence:

>1707_bases
ATGAACTTATTTAAAGAAAAAAAAATAAAAAAAATAATTTATATAAATTGGCGTTTTGTCACATTATGCAGTATTGTTTT
TTTATTTTTAGTAATTTTAACATTACGCATAATATTTCTTCAAATAATAAATTCTAAAAAATTAGCATACGAAGGAGATC
GTAGAACACTAAGAATACAATCAGTAATAAATAGAAGAGGCATAATTAATGATCGATTAGGATACCCATTAGCTGTAGCT
GTTCCAGTCAATGCAGTATTTATAGATCCCACAATGATTACCAATAAAAACGATATAAAAAATAACATACGTTGGAAAGC
ATTATCAGAGATACTATCTATTCCATTAAATAAATTAATATTTTTTATTAATTCAGATAAAAATATTAAGTTTATTTATT
TAGCTCGTCAAATTAATCCAGAAATCGGAGATTATATTAAAGCATTAAAATTACCAGGAGTTTTTTTGATAGAAGAATCA
AAACGATACTATCCTACTGGTGCTATCGCAGCTCAATTAATTGGGATAAATAATATAGATGGAGAGGGAATTGAAGGTAT
AGAAAAAAGTTTTAATTCATATTTAACAGGCACACCAGGTAAAAGAAAAATAAGAAAAGATAATCAAGGACAAATAATCG
AAAACGAATCTTTAATTAATAAATCCAATTCTAATAATTTAATACTCAGTATTGACAAAAAATTACAAACAATCGTATAT
CAAAAATTGAATAATGCTGTTAACGAAAATCAAGCAGATTTTGGAATTGCAATTTTAATTAATATTGAAACTGGAGAAAT
ATTAGCTATGGCTAATAGCCCTTCATATAATCCAAATAATATGCAATACATGATTAACAAAAATCTTCGTAATAAAGCTA
TTACAGATATTTTTGAACCAGGATCGACAGTTAAACCAATAGTTATTATGGAAGCATTAAAAAGAGGAATAATTAAAAAA
AACTCAATAATTAACACAAAACCTTATTTTATAAAAAAACATAAAATAACAGATGTTGCATACCATGAAAAATTAAATAT
AACAGGAATATTAAAAAAATCTAGTAATGTTGGGGTATCAAAAATTGCATTATCTATGAATACCTCAGAACTGATTAATA
GCTATATTAAATTTGGATTAGGACAGCCAACTAATTTAGGATTAATCGGAGAACAAAAAGGTTTTCTTCCTAAGAAAAAA
AAATTATCAGATCTAGAAAAAGCGACTTTTTCTTTTGGATACGGACTTATGATAACTCCTCTGCAATTAGCTCGTCTATA
TACAATTATTGGAAGCTATGGAATTTATCGTCCTCTTTCAATTATTAAAATTGATCATCCGTTATATGAAAAAAGAATTT
TTCCTAAAAGGTATGTAAAAAATGTTATCCATATGATGGAAACTGTTGCTCACCCTGGAGAAGGTGGTTCTCAAGCAGCT
ATTAAAGGATATCGTGTTGCAATTAAAACAGGAACTGCGAAAAAAGTAGGTATTCACGGATACTATATCAAAAAATATAT
AGCCTATACTGCAGGAATAGCACCAGCTAGCAATCCAAAATTTTCTTTAACAATTATAATTGATAATCCTAAAGGAGAAA
AATATTATGGAGGTGCAGTATCTGCGCCAGTTTTTAGTAAAATAATGAAATTAGTACTGAAAGAAATGAAAATTAAACCA
GATAATTTAAAAAACAAATTATCGTAA

Upstream 100 bases:

>100_bases
ATGCTATTTCTATGCCTTCTATTCATTAAAAATCTATATTTTAAATCAAATAAAAAATATGTCTAAAATATATAGATAGA
TAAAAATATAAACGAAAAAA

Downstream 100 bases:

>100_bases
TATTCTAAAAAAATCAATGAAAAAAAATAACTTAAAATATATTCTATTTCCATGGATTAAAAACACTCCTCAAAAAAAAT
TTTCAAATCTATCCTTAGAT

Product: penicillin binding protein 3 precursor

Products: NA

Alternate protein names: Penicillin-binding protein 3; PBP-3; Peptidoglycan glycosyltransferase 3

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MNLFKEKKIKKIIYINWRFVTLCSIVFLFLVILTLRIIFLQIINSKKLAYEGDRRTLRIQSVINRRGIINDRLGYPLAVA
VPVNAVFIDPTMITNKNDIKNNIRWKALSEILSIPLNKLIFFINSDKNIKFIYLARQINPEIGDYIKALKLPGVFLIEES
KRYYPTGAIAAQLIGINNIDGEGIEGIEKSFNSYLTGTPGKRKIRKDNQGQIIENESLINKSNSNNLILSIDKKLQTIVY
QKLNNAVNENQADFGIAILINIETGEILAMANSPSYNPNNMQYMINKNLRNKAITDIFEPGSTVKPIVIMEALKRGIIKK
NSIINTKPYFIKKHKITDVAYHEKLNITGILKKSSNVGVSKIALSMNTSELINSYIKFGLGQPTNLGLIGEQKGFLPKKK
KLSDLEKATFSFGYGLMITPLQLARLYTIIGSYGIYRPLSIIKIDHPLYEKRIFPKRYVKNVIHMMETVAHPGEGGSQAA
IKGYRVAIKTGTAKKVGIHGYYIKKYIAYTAGIAPASNPKFSLTIIIDNPKGEKYYGGAVSAPVFSKIMKLVLKEMKIKP
DNLKNKLS

Sequences:

>Translated_568_residues
MNLFKEKKIKKIIYINWRFVTLCSIVFLFLVILTLRIIFLQIINSKKLAYEGDRRTLRIQSVINRRGIINDRLGYPLAVA
VPVNAVFIDPTMITNKNDIKNNIRWKALSEILSIPLNKLIFFINSDKNIKFIYLARQINPEIGDYIKALKLPGVFLIEES
KRYYPTGAIAAQLIGINNIDGEGIEGIEKSFNSYLTGTPGKRKIRKDNQGQIIENESLINKSNSNNLILSIDKKLQTIVY
QKLNNAVNENQADFGIAILINIETGEILAMANSPSYNPNNMQYMINKNLRNKAITDIFEPGSTVKPIVIMEALKRGIIKK
NSIINTKPYFIKKHKITDVAYHEKLNITGILKKSSNVGVSKIALSMNTSELINSYIKFGLGQPTNLGLIGEQKGFLPKKK
KLSDLEKATFSFGYGLMITPLQLARLYTIIGSYGIYRPLSIIKIDHPLYEKRIFPKRYVKNVIHMMETVAHPGEGGSQAA
IKGYRVAIKTGTAKKVGIHGYYIKKYIAYTAGIAPASNPKFSLTIIIDNPKGEKYYGGAVSAPVFSKIMKLVLKEMKIKP
DNLKNKLS
>Mature_568_residues
MNLFKEKKIKKIIYINWRFVTLCSIVFLFLVILTLRIIFLQIINSKKLAYEGDRRTLRIQSVINRRGIINDRLGYPLAVA
VPVNAVFIDPTMITNKNDIKNNIRWKALSEILSIPLNKLIFFINSDKNIKFIYLARQINPEIGDYIKALKLPGVFLIEES
KRYYPTGAIAAQLIGINNIDGEGIEGIEKSFNSYLTGTPGKRKIRKDNQGQIIENESLINKSNSNNLILSIDKKLQTIVY
QKLNNAVNENQADFGIAILINIETGEILAMANSPSYNPNNMQYMINKNLRNKAITDIFEPGSTVKPIVIMEALKRGIIKK
NSIINTKPYFIKKHKITDVAYHEKLNITGILKKSSNVGVSKIALSMNTSELINSYIKFGLGQPTNLGLIGEQKGFLPKKK
KLSDLEKATFSFGYGLMITPLQLARLYTIIGSYGIYRPLSIIKIDHPLYEKRIFPKRYVKNVIHMMETVAHPGEGGSQAA
IKGYRVAIKTGTAKKVGIHGYYIKKYIAYTAGIAPASNPKFSLTIIIDNPKGEKYYGGAVSAPVFSKIMKLVLKEMKIKP
DNLKNKLS

Specific function: Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786272, Length=550, Percent_Identity=57.4545454545455, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI1786854, Length=641, Percent_Identity=22.7769110764431, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FTSI_BUCAP (O85297)

Other databases:

- EMBL:   AF060492
- EMBL:   AE013218
- RefSeq:   NP_660565.1
- ProteinModelPortal:   O85297
- EnsemblBacteria:   EBBUCT00000000242
- GeneID:   1005415
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg216
- GeneTree:   EBGT00050000007834
- HOGENOM:   HBG712588
- OMA:   RTRVYVD
- ProtClustDB:   CLSK315801
- BioCyc:   BAPH198804:BUSG216-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase; SSF56519 PBP_dimer; SSF56601 PBP_transp_fold

EC number: =2.4.1.129

Molecular weight: Translated: 63940; Mature: 63940

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: ACT_SITE 302-302

Signals:

None

Transmembrane regions:

HASH(0xbf22f4c)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFKEKKIKKIIYINWRFVTLCSIVFLFLVILTLRIIFLQIINSKKLAYEGDRRTLRIQ
CCCCHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEHH
SVINRRGIINDRLGYPLAVAVPVNAVFIDPTMITNKNDIKNNIRWKALSEILSIPLNKLI
HHHHHCCCCCCCCCCCEEEEEECEEEEECCEEECCCHHHHHCHHHHHHHHHHHCCCCCEE
FFINSDKNIKFIYLARQINPEIGDYIKALKLPGVFLIEESKRYYPTGAIAAQLIGINNID
EEEECCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHEEECCCCC
GEGIEGIEKSFNSYLTGTPGKRKIRKDNQGQIIENESLINKSNSNNLILSIDKKLQTIVY
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCHHHCCCCCCCEEEEECHHHHHHHH
QKLNNAVNENQADFGIAILINIETGEILAMANSPSYNPNNMQYMINKNLRNKAITDIFEP
HHHHHHCCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHCC
GSTVKPIVIMEALKRGIIKKNSIINTKPYFIKKHKITDVAYHEKLNITGILKKSSNVGVS
CCCCCHHHHHHHHHHCCCCCCCEECCCCCEEEEECEEEEHHHCCCCEEEEEECCCCCCEE
KIALSMNTSELINSYIKFGLGQPTNLGLIGEQKGFLPKKKKLSDLEKATFSFGYGLMITP
EEEEECCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEHH
LQLARLYTIIGSYGIYRPLSIIKIDHPLYEKRIFPKRYVKNVIHMMETVAHPGEGGSQAA
HHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCE
IKGYRVAIKTGTAKKVGIHGYYIKKYIAYTAGIAPASNPKFSLTIIIDNPKGEKYYGGAV
EEEEEEEEEECCCCEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCEECCCC
SAPVFSKIMKLVLKEMKIKPDNLKNKLS
CHHHHHHHHHHHHHHHCCCCCCHHCCCH
>Mature Secondary Structure
MNLFKEKKIKKIIYINWRFVTLCSIVFLFLVILTLRIIFLQIINSKKLAYEGDRRTLRIQ
CCCCHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEHH
SVINRRGIINDRLGYPLAVAVPVNAVFIDPTMITNKNDIKNNIRWKALSEILSIPLNKLI
HHHHHCCCCCCCCCCCEEEEEECEEEEECCEEECCCHHHHHCHHHHHHHHHHHCCCCCEE
FFINSDKNIKFIYLARQINPEIGDYIKALKLPGVFLIEESKRYYPTGAIAAQLIGINNID
EEEECCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHEEECCCCC
GEGIEGIEKSFNSYLTGTPGKRKIRKDNQGQIIENESLINKSNSNNLILSIDKKLQTIVY
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCHHHCCCCCCCEEEEECHHHHHHHH
QKLNNAVNENQADFGIAILINIETGEILAMANSPSYNPNNMQYMINKNLRNKAITDIFEP
HHHHHHCCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHCC
GSTVKPIVIMEALKRGIIKKNSIINTKPYFIKKHKITDVAYHEKLNITGILKKSSNVGVS
CCCCCHHHHHHHHHHCCCCCCCEECCCCCEEEEECEEEEHHHCCCCEEEEEECCCCCCEE
KIALSMNTSELINSYIKFGLGQPTNLGLIGEQKGFLPKKKKLSDLEKATFSFGYGLMITP
EEEEECCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEHH
LQLARLYTIIGSYGIYRPLSIIKIDHPLYEKRIFPKRYVKNVIHMMETVAHPGEGGSQAA
HHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCE
IKGYRVAIKTGTAKKVGIHGYYIKKYIAYTAGIAPASNPKFSLTIIIDNPKGEKYYGGAV
EEEEEEEEEECCCCEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCEECCCC
SAPVFSKIMKLVLKEMKIKPDNLKNKLS
CHHHHHHHHHHHHHHHCCCCCCHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9688822; 12089438