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The map label for this gene is murD

Identifier: 21672497

GI number: 21672497

Start: 240919

End: 242241

Strand: Reverse

Name: murD

Synonym: BUsg212

Alternate gene names: 21672497

Gene position: 242241-240919 (Counterclockwise)

Preceding gene: 21672498

Following gene: 21672496

Centisome position: 37.76

GC content: 23.89

Gene sequence:

>1323_bases
ATGTCGTATAATTATTTTGGAAAAAAAATACTAATTTTAGGATTGGGATTGACTGGAATATCATGTATTAACTTTTTTCT
AAAAAAAGGAATACAACCTCGAGTAATAGATGAATCTAATAAACCTATTTTTTTAAATAAAATTCCCAAAAATATTGAAT
ACAAGTTAGGAAATTTAAAAGAAAATTGGATTTTAGAATCTGATTTAATTATTATCAGTCCAGGTATTTCTTCTTTTAAA
CCTATTTTAATGAAAGCCCGTTCATTAGGCATCGATATCATTAGCGATATTGAATTATTTTCTAGAGAAACAAAATGTCC
AATCATTTCTATTACTGGTACTAATGGCAAAAGTACTGTGGCAACTATGGTAAAAAAAATTGCAGAAAAATCAGGATATA
AAGTTTTATTAGGAGGAAATATTGGATTCCCAGTATTAGAAATGCTCAATAAAAAAGCTAGTTTATATGTACTTGAATTA
TCTAGTTTTCAATTAGAAACTACATTTAATTTGAAGTCAAAAATAGCTGTAGTTCTCAATATTACTGAAGATCATCTTGA
TAGATATCCAGAAGGTTTTGAACAATATAAAAAAACTAAATTGTCTATTTATAATAAAGCTAAAATTTGTTTAATTAAAT
TAAAAAAAGGAGAAAAAAAACCATTTAACACAAAATCAAAAAAATATATTAGTTTTGGAACGTGTAATAATAATGATTAT
TATATTAATTATGAAAAAGAAAAAGCAATTTTATTCCATAAAAACAAAAAAATAGTTGATACTAGTAACATTTTATTAAA
TGGACATCATAACTATGAAAATATACTTACATCATTAGCAATTTCAGACCAAATGAAATTTGATCAAAAAGTTTCAATAA
ATGTACTTAAAAAATTTTTAGGATTACCACATAGATTTCAAACAGTACACATTAATAATAATATATCCTGGATTAATGAT
TCTAAATCCACAAATGTTGACAGTACAAAAGCTGCTCTTAAAAATTTAAAGATAAAGGGAACAATATGGTTATTGCTAGG
AGGAGATGGAAAATCTTCTAATTTCAATATTTTAAAAAAATATTTCGAAAAAATAAAAATAAAAATATATTGTTTTGGAA
AGGATGGTCTTAATCTATCAAAACTATGCAAAAAAAAATCTATCTATACTAAAACTTTAAAACAAGCCATAATTTTAATA
TCAAAAAAAATTCAACCAGGTGATGTTGTTCTTTTATCACCAGGATGCAGCAGTAAAGATCAATTTTCTAATTTTGAAGA
AAGAGGGAATCTTTTCATAAAATTATCCAAGGAAATAAATTAA

Upstream 100 bases:

>100_bases
ATGAAATACAAGGATTATCAGAATCTGTCATTGTCGTAAGATTTTGGATTATTTCTATGCTGCTTTTATTCATAGGAATA
CTATCTTTAAAGGTAAATTA

Downstream 100 bases:

>100_bases
TGTTTTTTTTAAAAAAAAAAACTATATTGTATTATACGATCGTGTATTAGTATGGTTAACTTTAGGTTTATGTATAACAG
GATTAGTGATGGTAACTTCT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSYNYFGKKILILGLGLTGISCINFFLKKGIQPRVIDESNKPIFLNKIPKNIEYKLGNLKENWILESDLIIISPGISSFK
PILMKARSLGIDIISDIELFSRETKCPIISITGTNGKSTVATMVKKIAEKSGYKVLLGGNIGFPVLEMLNKKASLYVLEL
SSFQLETTFNLKSKIAVVLNITEDHLDRYPEGFEQYKKTKLSIYNKAKICLIKLKKGEKKPFNTKSKKYISFGTCNNNDY
YINYEKEKAILFHKNKKIVDTSNILLNGHHNYENILTSLAISDQMKFDQKVSINVLKKFLGLPHRFQTVHINNNISWIND
SKSTNVDSTKAALKNLKIKGTIWLLLGGDGKSSNFNILKKYFEKIKIKIYCFGKDGLNLSKLCKKKSIYTKTLKQAIILI
SKKIQPGDVVLLSPGCSSKDQFSNFEERGNLFIKLSKEIN

Sequences:

>Translated_440_residues
MSYNYFGKKILILGLGLTGISCINFFLKKGIQPRVIDESNKPIFLNKIPKNIEYKLGNLKENWILESDLIIISPGISSFK
PILMKARSLGIDIISDIELFSRETKCPIISITGTNGKSTVATMVKKIAEKSGYKVLLGGNIGFPVLEMLNKKASLYVLEL
SSFQLETTFNLKSKIAVVLNITEDHLDRYPEGFEQYKKTKLSIYNKAKICLIKLKKGEKKPFNTKSKKYISFGTCNNNDY
YINYEKEKAILFHKNKKIVDTSNILLNGHHNYENILTSLAISDQMKFDQKVSINVLKKFLGLPHRFQTVHINNNISWIND
SKSTNVDSTKAALKNLKIKGTIWLLLGGDGKSSNFNILKKYFEKIKIKIYCFGKDGLNLSKLCKKKSIYTKTLKQAIILI
SKKIQPGDVVLLSPGCSSKDQFSNFEERGNLFIKLSKEIN
>Mature_439_residues
SYNYFGKKILILGLGLTGISCINFFLKKGIQPRVIDESNKPIFLNKIPKNIEYKLGNLKENWILESDLIIISPGISSFKP
ILMKARSLGIDIISDIELFSRETKCPIISITGTNGKSTVATMVKKIAEKSGYKVLLGGNIGFPVLEMLNKKASLYVLELS
SFQLETTFNLKSKIAVVLNITEDHLDRYPEGFEQYKKTKLSIYNKAKICLIKLKKGEKKPFNTKSKKYISFGTCNNNDYY
INYEKEKAILFHKNKKIVDTSNILLNGHHNYENILTSLAISDQMKFDQKVSINVLKKFLGLPHRFQTVHINNNISWINDS
KSTNVDSTKAALKNLKIKGTIWLLLGGDGKSSNFNILKKYFEKIKIKIYCFGKDGLNLSKLCKKKSIYTKTLKQAIILIS
KKIQPGDVVLLSPGCSSKDQFSNFEERGNLFIKLSKEIN

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=436, Percent_Identity=47.2477064220183, Blast_Score=453, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_BUCAP (Q8K9T2)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660564.1
- ProteinModelPortal:   Q8K9T2
- SMR:   Q8K9T2
- EnsemblBacteria:   EBBUCT00000000148
- GeneID:   1005411
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg212
- GeneTree:   EBGT00050000007738
- HOGENOM:   HBG750024
- OMA:   LWVDDSK
- ProtClustDB:   PRK03806
- BioCyc:   BAPH198804:BUSG212-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49855; Mature: 49724

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYNYFGKKILILGLGLTGISCINFFLKKGIQPRVIDESNKPIFLNKIPKNIEYKLGNLK
CCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCEEECCCCCCEEEECCCCCCEEEECCCC
ENWILESDLIIISPGISSFKPILMKARSLGIDIISDIELFSRETKCPIISITGTNGKSTV
CCEEEECCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHH
ATMVKKIAEKSGYKVLLGGNIGFPVLEMLNKKASLYVLELSSFQLETTFNLKSKIAVVLN
HHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCEEEEEEECCEEEEEEECCCCEEEEEEE
ITEDHLDRYPEGFEQYKKTKLSIYNKAKICLIKLKKGEKKPFNTKSKKYISFGTCNNNDY
ECHHHHHCCCHHHHHHHHHHEEEECCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCCE
YINYEKEKAILFHKNKKIVDTSNILLNGHHNYENILTSLAISDQMKFDQKVSINVLKKFL
EEEEECCEEEEEECCCEEEECCCEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
GLPHRFQTVHINNNISWINDSKSTNVDSTKAALKNLKIKGTIWLLLGGDGKSSNFNILKK
CCCCEEEEEEECCCEEEECCCCCCCCCHHHHHHHCEEEEEEEEEEECCCCCCCCHHHHHH
YFEKIKIKIYCFGKDGLNLSKLCKKKSIYTKTLKQAIILISKKIQPGDVVLLSPGCSSKD
HHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHH
QFSNFEERGNLFIKLSKEIN
HHCCHHHCCCEEEEEECCCC
>Mature Secondary Structure 
SYNYFGKKILILGLGLTGISCINFFLKKGIQPRVIDESNKPIFLNKIPKNIEYKLGNLK
CCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCEEECCCCCCEEEECCCCCCEEEECCCC
ENWILESDLIIISPGISSFKPILMKARSLGIDIISDIELFSRETKCPIISITGTNGKSTV
CCEEEECCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHH
ATMVKKIAEKSGYKVLLGGNIGFPVLEMLNKKASLYVLELSSFQLETTFNLKSKIAVVLN
HHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCEEEEEEECCEEEEEEECCCCEEEEEEE
ITEDHLDRYPEGFEQYKKTKLSIYNKAKICLIKLKKGEKKPFNTKSKKYISFGTCNNNDY
ECHHHHHCCCHHHHHHHHHHEEEECCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCCE
YINYEKEKAILFHKNKKIVDTSNILLNGHHNYENILTSLAISDQMKFDQKVSINVLKKFL
EEEEECCEEEEEECCCEEEECCCEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
GLPHRFQTVHINNNISWINDSKSTNVDSTKAALKNLKIKGTIWLLLGGDGKSSNFNILKK
CCCCEEEEEEECCCEEEECCCCCCCCCHHHHHHHCEEEEEEEEEEECCCCCCCCHHHHHH
YFEKIKIKIYCFGKDGLNLSKLCKKKSIYTKTLKQAIILISKKIQPGDVVLLSPGCSSKD
HHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHH
QFSNFEERGNLFIKLSKEIN
HHCCHHHCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438