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The map label for this gene is thrC

Identifier: 21672473

GI number: 21672473

Start: 207801

End: 209090

Strand: Reverse

Name: thrC

Synonym: BUsg186

Alternate gene names: 21672473

Gene position: 209090-207801 (Counterclockwise)

Preceding gene: 21672474

Following gene: 21672469

Centisome position: 32.6

GC content: 27.75

Gene sequence:

>1290_bases
ATGAAACTCTATAATTTAAAAAACCATAACGAACAAGTAAATTTTGAAACTGCCGTAAAACTTGGATTAGGACAGAAACA
AGGTTTGTTTTTTCCAGTAAAATTACCAATTATGACTCCTGTAGAGTTATCAAAAATATTAAAAATGGATTTTATTACAC
GAAGTACTGAAATACTTTCTAAATTTATTAGTAGTGAAATTTCTAAAGAAGTATTGCATGAACATGTTAAAAAAGCATTT
TCATTTTCAAAACCATTAAAAATTTGTATTAATAAGAATATAAGCTGTTTTGAACTATTTCATGGCCCAACATTAGCATT
TAAAGATTTTGGGGCACGTTTTATGGCTCAAATGATACTATGTCTTAATAAAAAAAATGAATCTTTTACGATTTTGACAG
CAACGTCAGGAGACACCGGAGCTGCAGTTGCTCATGCTTTTTATGGAATGAAAAACATTCGAGTAATTATTTTATATCCA
AAGGGAAAAATCACTCTATTACAAGAACAATTATTTTGTACTTTAGGGAAAAATATAAAAACTATTTCTATTAACGGTAG
TTTTGACGATTGTCAAAAATTAGTCAAAAAAGCCTTTGATGATAAAAAATTAAAAGAATCAATAGGATTAAATTCTGCTA
ATTCTATTAATATAAGTCGTTTATTAGCCCAAATATGCTATTATTTTGAAGCTTTTTCATTGATTTCTGAAGAAAAAAGA
AAAAATTTAGTTATAGCCGTTCCATGCGGTAACTTTGGTAATTTAACAGCTGGTTTATTAGCCAAATCTCTTGGTTTGCC
AATTCAATCATTTATTGCATGTACTAATTCTAATGATACAGTTCCAAGATTTCTGAATAGTGGAAAATGGAATCCTAAAA
AAACTGTATCAACAATTTCTAATGCTATGGATATTAGTTGCCCTAATAATTGGCCCCGAATTGAAGAATTATTTCGTCGT
AAAAAATGGGATTTAAAAGAATTAAGATTCGGAAGTGTATCAGATAATGTTACTAAAGAGACTCTTAAAGAGTTATTTCG
AATGGGTTATGTATCCGAACCACATGCTGCAATTGCCTACAGGCTATTGCATGATCAATTAAAAAAAGAAGAATTCGGTT
TGTTTTTAGGAACTGCACATCCGTCTAAATTTAAAGATACTGTAGAAAAAATATTAGAAAACAGTATTTCTTTACCAAAA
GAATTGAAAAATAGAAATAATTTACCATTATTATCCCATAATATTAATCCTGATTTTAATAAATTAAAAGAATTTTTATT
AGAAAAATAA

Upstream 100 bases:

>100_bases
GATATCTTTATGGCTAAAAAATAATTATTTACAAAATAAAACAGGATTTGTTCACATTTGTGCTGTAGATGTAAAAGGTG
TGCGAAAAATAGGATAAAAA

Downstream 100 bases:

>100_bases
TCATATAAATTAGAGAGGATAAATTAAATTTTCCTCTCTAATAAAAAATAACTTTTTTTAAACATTAAATAAAAAATTAA
TAATGTCGCCATCTTGAATT

Product: threonine synthase

Products: NA

Alternate protein names: TS

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKLYNLKNHNEQVNFETAVKLGLGQKQGLFFPVKLPIMTPVELSKILKMDFITRSTEILSKFISSEISKEVLHEHVKKAF
SFSKPLKICINKNISCFELFHGPTLAFKDFGARFMAQMILCLNKKNESFTILTATSGDTGAAVAHAFYGMKNIRVIILYP
KGKITLLQEQLFCTLGKNIKTISINGSFDDCQKLVKKAFDDKKLKESIGLNSANSINISRLLAQICYYFEAFSLISEEKR
KNLVIAVPCGNFGNLTAGLLAKSLGLPIQSFIACTNSNDTVPRFLNSGKWNPKKTVSTISNAMDISCPNNWPRIEELFRR
KKWDLKELRFGSVSDNVTKETLKELFRMGYVSEPHAAIAYRLLHDQLKKEEFGLFLGTAHPSKFKDTVEKILENSISLPK
ELKNRNNLPLLSHNINPDFNKLKEFLLEK

Sequences:

>Translated_429_residues
MKLYNLKNHNEQVNFETAVKLGLGQKQGLFFPVKLPIMTPVELSKILKMDFITRSTEILSKFISSEISKEVLHEHVKKAF
SFSKPLKICINKNISCFELFHGPTLAFKDFGARFMAQMILCLNKKNESFTILTATSGDTGAAVAHAFYGMKNIRVIILYP
KGKITLLQEQLFCTLGKNIKTISINGSFDDCQKLVKKAFDDKKLKESIGLNSANSINISRLLAQICYYFEAFSLISEEKR
KNLVIAVPCGNFGNLTAGLLAKSLGLPIQSFIACTNSNDTVPRFLNSGKWNPKKTVSTISNAMDISCPNNWPRIEELFRR
KKWDLKELRFGSVSDNVTKETLKELFRMGYVSEPHAAIAYRLLHDQLKKEEFGLFLGTAHPSKFKDTVEKILENSISLPK
ELKNRNNLPLLSHNINPDFNKLKEFLLEK
>Mature_429_residues
MKLYNLKNHNEQVNFETAVKLGLGQKQGLFFPVKLPIMTPVELSKILKMDFITRSTEILSKFISSEISKEVLHEHVKKAF
SFSKPLKICINKNISCFELFHGPTLAFKDFGARFMAQMILCLNKKNESFTILTATSGDTGAAVAHAFYGMKNIRVIILYP
KGKITLLQEQLFCTLGKNIKTISINGSFDDCQKLVKKAFDDKKLKESIGLNSANSINISRLLAQICYYFEAFSLISEEKR
KNLVIAVPCGNFGNLTAGLLAKSLGLPIQSFIACTNSNDTVPRFLNSGKWNPKKTVSTISNAMDISCPNNWPRIEELFRR
KKWDLKELRFGSVSDNVTKETLKELFRMGYVSEPHAAIAYRLLHDQLKKEEFGLFLGTAHPSKFKDTVEKILENSISLPK
ELKNRNNLPLLSHNINPDFNKLKEFLLEK

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family

Homologues:

Organism=Homo sapiens, GI149193323, Length=418, Percent_Identity=29.9043062200957, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI153792148, Length=456, Percent_Identity=28.9473684210526, Blast_Score=150, Evalue=3e-36,
Organism=Escherichia coli, GI1786185, Length=427, Percent_Identity=62.7634660421546, Blast_Score=574, Evalue=1e-165,
Organism=Saccharomyces cerevisiae, GI6319901, Length=445, Percent_Identity=32.3595505617978, Blast_Score=209, Evalue=5e-55,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): THRC_BUCAP (Q8K9V1)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660540.1
- ProteinModelPortal:   Q8K9V1
- SMR:   Q8K9V1
- EnsemblBacteria:   EBBUCT00000000004
- GeneID:   1005383
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg186
- GeneTree:   EBGT00050000008192
- HOGENOM:   HBG521025
- OMA:   CINAFAQ
- ProtClustDB:   PRK09225
- BioCyc:   BAPH198804:BUSG186-MONOMER
- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450
- TIGRFAMs:   TIGR00260

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.3.1

Molecular weight: Translated: 48585; Mature: 48585

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYNLKNHNEQVNFETAVKLGLGQKQGLFFPVKLPIMTPVELSKILKMDFITRSTEILS
CCCCCCCCCCCCCCHHHHHEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KFISSEISKEVLHEHVKKAFSFSKPLKICINKNISCFELFHGPTLAFKDFGARFMAQMIL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
CLNKKNESFTILTATSGDTGAAVAHAFYGMKNIRVIILYPKGKITLLQEQLFCTLGKNIK
HHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHHHHCCCEE
TISINGSFDDCQKLVKKAFDDKKLKESIGLNSANSINISRLLAQICYYFEAFSLISEEKR
EEEECCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KNLVIAVPCGNFGNLTAGLLAKSLGLPIQSFIACTNSNDTVPRFLNSGKWNPKKTVSTIS
CCEEEEEECCCCCCHHHHHHHHHHCCCHHHHEEECCCCCCHHHHHHCCCCCHHHHHHHHH
NAMDISCPNNWPRIEELFRRKKWDLKELRFGSVSDNVTKETLKELFRMGYVSEPHAAIAY
HHEECCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
RLLHDQLKKEEFGLFLGTAHPSKFKDTVEKILENSISLPKELKNRNNLPLLSHNINPDFN
HHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCHH
KLKEFLLEK
HHHHHHCCC
>Mature Secondary Structure
MKLYNLKNHNEQVNFETAVKLGLGQKQGLFFPVKLPIMTPVELSKILKMDFITRSTEILS
CCCCCCCCCCCCCCHHHHHEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KFISSEISKEVLHEHVKKAFSFSKPLKICINKNISCFELFHGPTLAFKDFGARFMAQMIL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
CLNKKNESFTILTATSGDTGAAVAHAFYGMKNIRVIILYPKGKITLLQEQLFCTLGKNIK
HHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHHHHCCCEE
TISINGSFDDCQKLVKKAFDDKKLKESIGLNSANSINISRLLAQICYYFEAFSLISEEKR
EEEECCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KNLVIAVPCGNFGNLTAGLLAKSLGLPIQSFIACTNSNDTVPRFLNSGKWNPKKTVSTIS
CCEEEEEECCCCCCHHHHHHHHHHCCCHHHHEEECCCCCCHHHHHHCCCCCHHHHHHHHH
NAMDISCPNNWPRIEELFRRKKWDLKELRFGSVSDNVTKETLKELFRMGYVSEPHAAIAY
HHEECCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
RLLHDQLKKEEFGLFLGTAHPSKFKDTVEKILENSISLPKELKNRNNLPLLSHNINPDFN
HHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCHH
KLKEFLLEK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438