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The map label for this gene is lspA

Identifier: 21672431

GI number: 21672431

Start: 156895

End: 157365

Strand: Reverse

Name: lspA

Synonym: BUsg141

Alternate gene names: 21672431

Gene position: 157365-156895 (Counterclockwise)

Preceding gene: 21672432

Following gene: 21672430

Centisome position: 24.53

GC content: 20.38

Gene sequence:

>471_bases
ATGAAAAGAAAATACTATTGGATATATATAAATATTATATTTTTTATCATAACAGTAGATTTTTATAGTAAAAAATGGAT
TTTAAATCATTTAAATATATATGAAAAACAAAAAGTATTTTTCATATTAAATTTATTTCATGTCCATAATTTTGGAGCAG
CATTCAGCATCTTGTCAGATCAAAACGGATGGCAAAAATATTTCCTGTTAATATTTAGTATAATTATTATATTAGCAATT
ATAAAAATAATGATAAAATTTAAAAAAAAAGATAAAAATAAAATTCTTTCTTATTCTCTCATTCTCGCAGGTGCAATAGG
AAATTTAATAGATCGTATTAACTATGGATTTGTAATAGATTTTATTGATTTACACTTTAAAAGTTGGCATTTTGCAACCT
TTAATATAGCTGATTTCAGTATATTTATTGGCATGATTATGATCATAAAAAAAAATTATTATAATTCGTAA

Upstream 100 bases:

>100_bases
GATGCTGGCATTATAATATTTTTATTGAAAATAATAATGATAAAATTTGTACACGTTGTATTTTAAATACTAAAGGAAAT
GGTGAAAAGCGCTTTTTTAT

Downstream 100 bases:

>100_bases
TGAATCTATAATGAATTTTAATTATCAATTAAATAAATTTTGTTTGTAATAAAAATACAGTTTGAGAAAAATAGTGAATA
TTATATTAGCCAAACCAAGA

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKRKYYWIYINIIFFIITVDFYSKKWILNHLNIYEKQKVFFILNLFHVHNFGAAFSILSDQNGWQKYFLLIFSIIIILAI
IKIMIKFKKKDKNKILSYSLILAGAIGNLIDRINYGFVIDFIDLHFKSWHFATFNIADFSIFIGMIMIIKKNYYNS

Sequences:

>Translated_156_residues
MKRKYYWIYINIIFFIITVDFYSKKWILNHLNIYEKQKVFFILNLFHVHNFGAAFSILSDQNGWQKYFLLIFSIIIILAI
IKIMIKFKKKDKNKILSYSLILAGAIGNLIDRINYGFVIDFIDLHFKSWHFATFNIADFSIFIGMIMIIKKNYYNS
>Mature_156_residues
MKRKYYWIYINIIFFIITVDFYSKKWILNHLNIYEKQKVFFILNLFHVHNFGAAFSILSDQNGWQKYFLLIFSIIIILAI
IKIMIKFKKKDKNKILSYSLILAGAIGNLIDRINYGFVIDFIDLHFKSWHFATFNIADFSIFIGMIMIIKKNYYNS

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=150, Percent_Identity=38.6666666666667, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_BUCAP (Q8K9Z3)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660498.1
- MEROPS:   A08.001
- EnsemblBacteria:   EBBUCT00000000007
- GeneID:   1005958
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg141
- GeneTree:   EBGT00050000007886
- HOGENOM:   HBG724422
- OMA:   ADSSGWQ
- ProtClustDB:   PRK00376
- BioCyc:   BAPH198804:BUSG141-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 18749; Mature: 18749

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 111-111 ACT_SITE 138-138

Signals:

None

Transmembrane regions:

HASH(0xc0127d8)-; HASH(0xbff3fdc)-; HASH(0x96dc450)-; HASH(0xbe23e84)-; HASH(0xbff8b9c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKYYWIYINIIFFIITVDFYSKKWILNHLNIYEKQKVFFILNLFHVHNFGAAFSILSD
CCCEEEEEHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHEEHHHHHHHHCCCHHEEEECC
QNGWQKYFLLIFSIIIILAIIKIMIKFKKKDKNKILSYSLILAGAIGNLIDRINYGFVID
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
FIDLHFKSWHFATFNIADFSIFIGMIMIIKKNYYNS
EEEHHCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure
MKRKYYWIYINIIFFIITVDFYSKKWILNHLNIYEKQKVFFILNLFHVHNFGAAFSILSD
CCCEEEEEHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHEEHHHHHHHHCCCHHEEEECC
QNGWQKYFLLIFSIIIILAIIKIMIKFKKKDKNKILSYSLILAGAIGNLIDRINYGFVID
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
FIDLHFKSWHFATFNIADFSIFIGMIMIIKKNYYNS
EEEHHCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12089438