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The map label for this gene is ispH

Identifier: 21672430

GI number: 21672430

Start: 155880

End: 156821

Strand: Reverse

Name: ispH

Synonym: BUsg140

Alternate gene names: 21672430

Gene position: 156821-155880 (Counterclockwise)

Preceding gene: 21672431

Following gene: 21672429

Centisome position: 24.45

GC content: 25.8

Gene sequence:

>942_bases
GTGAATATTATATTAGCCAAACCAAGAGGTTTTTGCGCAGGTGTTAAAAGAGCTCTCTTAATAGTTGAAAATGCTTTAAA
AATTTATAAAAAAACTATTTATGTTCAGCATGAACTAGTACATAATCAATATGTTATAGAAACATTACGTCAAAAGGGAG
TTGTCTTCGTTGAAGATATTTCAGATATTCCAAATAATTCAATAGTAGTTTTTTCAGCACACGGAGTATCAAAAAAAATA
AAAAAAAAAGCTATAAAAAAAAATTTAACAATATTAAATGCCACTTGCCCATTAGTGACAAAAGTGCATTTAGAAGTTTC
TAAATCAAGTAAAAATGGCATAGAAACAATTCTAATTGGTCATAGAGGACATCCGGAAGTTATCGGTACAATTGGTCAAT
ATGATAATAAAAAGGGAAAAATACATCTTATTGAAGATATAGAAGATGTTAATCAATTAACAATTAAAATAGATAAAAAA
TTAAATTTTTTTACACAAACAACCTTATCTATCAAAAATACTCAATCTATTATTTTAGCTTTGAAAAAAAAATTTTTAAA
TATATCTGGTCCTAAAAAAGAGGATATATGCTATGCAACAACAAATAGACAAAATGCAATTATTAAATTATCTAAAATAA
CTGATATAATACTTGTAATTGGTTCTAAAAATTCTTCTAATTCTAGTCGTCTTTCAGAATTAGGAAAAGAAACAGGTGTA
TTTACGAAACAAATTGAATCATTTTCTGATATTAAAGAAGAATGGCTAAAAAATAAAAAAAATATTGGCATTACAGCTGG
TGCTTCCGCACCGGATATATTAGTAGAGGAAGTAATCAAACATTTAAAAAAACTAGGTTTTAAAAATCCACCAAAAGAAA
TGTTAGGAGATGAAGAAAAAACAATTTTTAAAATACCCAAAAATTTAAACGTACAAAATTAG

Upstream 100 bases:

>100_bases
ATAAAAAAAAATTATTATAATTCGTAATGAATCTATAATGAATTTTAATTATCAATTAAATAAATTTTGTTTGTAATAAA
AATACAGTTTGAGAAAAATA

Downstream 100 bases:

>100_bases
AAAAATACAATTATGAAAAAAAAAATAACTCGTATCGCAATTACTGGTGCTATGGGACGTATGGGCCAGGTATTAATTAA
AGAAATACAAAAAAATAAAA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNIILAKPRGFCAGVKRALLIVENALKIYKKTIYVQHELVHNQYVIETLRQKGVVFVEDISDIPNNSIVVFSAHGVSKKI
KKKAIKKNLTILNATCPLVTKVHLEVSKSSKNGIETILIGHRGHPEVIGTIGQYDNKKGKIHLIEDIEDVNQLTIKIDKK
LNFFTQTTLSIKNTQSIILALKKKFLNISGPKKEDICYATTNRQNAIIKLSKITDIILVIGSKNSSNSSRLSELGKETGV
FTKQIESFSDIKEEWLKNKKNIGITAGASAPDILVEEVIKHLKKLGFKNPPKEMLGDEEKTIFKIPKNLNVQN

Sequences:

>Translated_313_residues
MNIILAKPRGFCAGVKRALLIVENALKIYKKTIYVQHELVHNQYVIETLRQKGVVFVEDISDIPNNSIVVFSAHGVSKKI
KKKAIKKNLTILNATCPLVTKVHLEVSKSSKNGIETILIGHRGHPEVIGTIGQYDNKKGKIHLIEDIEDVNQLTIKIDKK
LNFFTQTTLSIKNTQSIILALKKKFLNISGPKKEDICYATTNRQNAIIKLSKITDIILVIGSKNSSNSSRLSELGKETGV
FTKQIESFSDIKEEWLKNKKNIGITAGASAPDILVEEVIKHLKKLGFKNPPKEMLGDEEKTIFKIPKNLNVQN
>Mature_313_residues
MNIILAKPRGFCAGVKRALLIVENALKIYKKTIYVQHELVHNQYVIETLRQKGVVFVEDISDIPNNSIVVFSAHGVSKKI
KKKAIKKNLTILNATCPLVTKVHLEVSKSSKNGIETILIGHRGHPEVIGTIGQYDNKKGKIHLIEDIEDVNQLTIKIDKK
LNFFTQTTLSIKNTQSIILALKKKFLNISGPKKEDICYATTNRQNAIIKLSKITDIILVIGSKNSSNSSRLSELGKETGV
FTKQIESFSDIKEEWLKNKKNIGITAGASAPDILVEEVIKHLKKLGFKNPPKEMLGDEEKTIFKIPKNLNVQN

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=312, Percent_Identity=54.4871794871795, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_BUCAP (Q8K9Z4)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660497.1
- EnsemblBacteria:   EBBUCT00000000115
- GeneID:   1005957
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg140
- GeneTree:   EBGT00050000008027
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- BioCyc:   BAPH198804:BUSG140-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34998; Mature: 34998

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIILAKPRGFCAGVKRALLIVENALKIYKKTIYVQHELVHNQYVIETLRQKGVVFVEDI
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
SDIPNNSIVVFSAHGVSKKIKKKAIKKNLTILNATCPLVTKVHLEVSKSSKNGIETILIG
CCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEECCCCEEEEEEEEEECCCCCCCEEEEEE
HRGHPEVIGTIGQYDNKKGKIHLIEDIEDVNQLTIKIDKKLNFFTQTTLSIKNTQSIILA
CCCCHHHEECCCCCCCCCCCEEEECCHHCCCEEEEEEECCCCEEEEEEEEECCHHHHHHH
LKKKFLNISGPKKEDICYATTNRQNAIIKLSKITDIILVIGSKNSSNSSRLSELGKETGV
HHHHHHCCCCCCCCCEEEEECCCCCEEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHCH
FTKQIESFSDIKEEWLKNKKNIGITAGASAPDILVEEVIKHLKKLGFKNPPKEMLGDEEK
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCC
TIFKIPKNLNVQN
EEEECCCCCCCCC
>Mature Secondary Structure
MNIILAKPRGFCAGVKRALLIVENALKIYKKTIYVQHELVHNQYVIETLRQKGVVFVEDI
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
SDIPNNSIVVFSAHGVSKKIKKKAIKKNLTILNATCPLVTKVHLEVSKSSKNGIETILIG
CCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEECCCCEEEEEEEEEECCCCCCCEEEEEE
HRGHPEVIGTIGQYDNKKGKIHLIEDIEDVNQLTIKIDKKLNFFTQTTLSIKNTQSIILA
CCCCHHHEECCCCCCCCCCCEEEECCHHCCCEEEEEEECCCCEEEEEEEEECCHHHHHHH
LKKKFLNISGPKKEDICYATTNRQNAIIKLSKITDIILVIGSKNSSNSSRLSELGKETGV
HHHHHHCCCCCCCCCEEEEECCCCCEEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHCH
FTKQIESFSDIKEEWLKNKKNIGITAGASAPDILVEEVIKHLKKLGFKNPPKEMLGDEEK
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCC
TIFKIPKNLNVQN
EEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438