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The map label for this gene is cysK

Identifier: 21672356

GI number: 21672356

Start: 71358

End: 72326

Strand: Reverse

Name: cysK

Synonym: BUsg063

Alternate gene names: 21672356

Gene position: 72326-71358 (Counterclockwise)

Preceding gene: 21672358

Following gene: 21672355

Centisome position: 11.28

GC content: 28.38

Gene sequence:

>969_bases
ATGAATAAAATATATGAAGATAATTCTTTCACTATTGGAAATACACCACTTGTTCGTTTAAACAATATAGGAAACGGAAA
CATTTTAGCAAAAATAGAATCTAGAAATCCAAGTTTTAGTGTGAAATGTAGAATTGGTGCTAATATGATATGGGACGCAG
AAAAAAAGGGACGTTTAAATAAAAACATAGAAATAATTGAAGCAACTAGTGGTAATACAGGAATAGCTTTAGCATATGTT
GCAGCGGCGAGAAATTATTCATTAACTCTTATAATGCCTGATTCTATGTCTATAGAAAGAAGAAAATTATTAAAATCTTT
AGGTGCACATTTAATACTAACAAATGGAAAACACGGTATGAAAGGTGCTATATCTAAAGCAAATGAAATTGTATCTTTAA
ATACAAAACGTTATCTTTTATTAAAACAATTTGAAAATCCAGCCAATCCAGAAATACATCAAAAAACCACTGGTCCAGAA
ATTTGGAAAGACACCAAAGGCAATATAGACATATTAATTTCAGGCGTAGGTACAGGAGGAACTATTACAGGAATTACAAA
ATATATAAAAATAAAAGAAGGGAAAAAAGACTTAATTAGCGTTGCCGTTGAACCTTTAGAATCACCTGTAATTACACAAT
TTTTATCAGGAAAAAAAATAAAACCAGGATTACATAAAATACAAGGAATAGGAGCAGGTTTTATTCCTAAAAATTTAGAT
TTATCATTAATTGACAGAATCATTACAGTATCCAGCGAAGAAGCAATATTTAATGCTAAAAAAATAATGAAAAAGGAAGG
AATATTATCAGGAATTTCTTCAGGTGCAGCTATAGCAGCGGCTTTAAAAATACAAAAAGAAAATGATTTTAAAAATAAAA
ATATAGTAGTTATATTACCTTCTTCAGGAGAGCGTTATTTAAGTACAGAGTTATTTTCTAGATCATTTAAAAATGAAAAC
AATTATTAA

Upstream 100 bases:

>100_bases
GTATAATGAAAAATCTAAAAAATCCTAATAAAGAACATTTAATAACATACTAAAAAATTTTAAATAAAAATAAAAATTAG
TATTAAGGAATTCAGAAAAA

Downstream 100 bases:

>100_bases
AAAAATAATACAACTTTTTTAATAAAAAAATAAATTAATTTTTATTATAAAAGTTGTATTTTAATATTTTAAATTAAAAT
TTATTTTAAAATATATTAAC

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNKIYEDNSFTIGNTPLVRLNNIGNGNILAKIESRNPSFSVKCRIGANMIWDAEKKGRLNKNIEIIEATSGNTGIALAYV
AAARNYSLTLIMPDSMSIERRKLLKSLGAHLILTNGKHGMKGAISKANEIVSLNTKRYLLLKQFENPANPEIHQKTTGPE
IWKDTKGNIDILISGVGTGGTITGITKYIKIKEGKKDLISVAVEPLESPVITQFLSGKKIKPGLHKIQGIGAGFIPKNLD
LSLIDRIITVSSEEAIFNAKKIMKKEGILSGISSGAAIAAALKIQKENDFKNKNIVVILPSSGERYLSTELFSRSFKNEN
NY

Sequences:

>Translated_322_residues
MNKIYEDNSFTIGNTPLVRLNNIGNGNILAKIESRNPSFSVKCRIGANMIWDAEKKGRLNKNIEIIEATSGNTGIALAYV
AAARNYSLTLIMPDSMSIERRKLLKSLGAHLILTNGKHGMKGAISKANEIVSLNTKRYLLLKQFENPANPEIHQKTTGPE
IWKDTKGNIDILISGVGTGGTITGITKYIKIKEGKKDLISVAVEPLESPVITQFLSGKKIKPGLHKIQGIGAGFIPKNLD
LSLIDRIITVSSEEAIFNAKKIMKKEGILSGISSGAAIAAALKIQKENDFKNKNIVVILPSSGERYLSTELFSRSFKNEN
NY
>Mature_322_residues
MNKIYEDNSFTIGNTPLVRLNNIGNGNILAKIESRNPSFSVKCRIGANMIWDAEKKGRLNKNIEIIEATSGNTGIALAYV
AAARNYSLTLIMPDSMSIERRKLLKSLGAHLILTNGKHGMKGAISKANEIVSLNTKRYLLLKQFENPANPEIHQKTTGPE
IWKDTKGNIDILISGVGTGGTITGITKYIKIKEGKKDLISVAVEPLESPVITQFLSGKKIKPGLHKIQGIGAGFIPKNLD
LSLIDRIITVSSEEAIFNAKKIMKKEGILSGISSGAAIAAALKIQKENDFKNKNIVVILPSSGERYLSTELFSRSFKNEN
NY

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family

Homologues:

Organism=Homo sapiens, GI295821202, Length=319, Percent_Identity=36.9905956112853, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI295821200, Length=319, Percent_Identity=36.9905956112853, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI4557415, Length=319, Percent_Identity=36.9905956112853, Blast_Score=182, Evalue=3e-46,
Organism=Escherichia coli, GI1788754, Length=316, Percent_Identity=72.1518987341772, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI2367138, Length=305, Percent_Identity=40.655737704918, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17535051, Length=314, Percent_Identity=48.7261146496815, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI115535073, Length=316, Percent_Identity=48.1012658227848, Blast_Score=251, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17562970, Length=315, Percent_Identity=44.4444444444444, Blast_Score=248, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI32566674, Length=309, Percent_Identity=43.3656957928803, Blast_Score=239, Evalue=9e-64,
Organism=Caenorhabditis elegans, GI17561720, Length=315, Percent_Identity=44.7619047619048, Blast_Score=230, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI32566672, Length=222, Percent_Identity=43.6936936936937, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17534315, Length=312, Percent_Identity=36.2179487179487, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI25147552, Length=327, Percent_Identity=34.2507645259939, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17561716, Length=127, Percent_Identity=40.1574803149606, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321594, Length=325, Percent_Identity=33.5384615384615, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321449, Length=322, Percent_Identity=36.0248447204969, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24643623, Length=319, Percent_Identity=34.4827586206897, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI20129101, Length=319, Percent_Identity=34.4827586206897, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSK_BUCAP (Q8KA48)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660423.1
- ProteinModelPortal:   Q8KA48
- SMR:   Q8KA48
- EnsemblBacteria:   EBBUCT00000000248
- GeneID:   1005879
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg063
- GeneTree:   EBGT00050000008139
- HOGENOM:   HBG748215
- OMA:   AHSIGNT
- ProtClustDB:   CLSK315135
- BioCyc:   BAPH198804:BUSG063-MONOMER
- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926
- TIGRFAMs:   TIGR01139
- TIGRFAMs:   TIGR01136

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =2.5.1.47

Molecular weight: Translated: 35177; Mature: 35177

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: BINDING 8-8 BINDING 72-72 BINDING 269-269 BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIYEDNSFTIGNTPLVRLNNIGNGNILAKIESRNPSFSVKCRIGANMIWDAEKKGRLN
CCCEECCCCEEECCCCEEEEECCCCCEEEEEEECCCCCEEEEEEECCCEEECCHHCCCCC
KNIEIIEATSGNTGIALAYVAAARNYSLTLIMPDSMSIERRKLLKSLGAHLILTNGKHGM
CCEEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCC
KGAISKANEIVSLNTKRYLLLKQFENPANPEIHQKTTGPEIWKDTKGNIDILISGVGTGG
CHHHHCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCEEEEEEECCCCC
TITGITKYIKIKEGKKDLISVAVEPLESPVITQFLSGKKIKPGLHKIQGIGAGFIPKNLD
CHHCEEEEEEEECCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCC
LSLIDRIITVSSEEAIFNAKKIMKKEGILSGISSGAAIAAALKIQKENDFKNKNIVVILP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCEEEEEC
SSGERYLSTELFSRSFKNENNY
CCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNKIYEDNSFTIGNTPLVRLNNIGNGNILAKIESRNPSFSVKCRIGANMIWDAEKKGRLN
CCCEECCCCEEECCCCEEEEECCCCCEEEEEEECCCCCEEEEEEECCCEEECCHHCCCCC
KNIEIIEATSGNTGIALAYVAAARNYSLTLIMPDSMSIERRKLLKSLGAHLILTNGKHGM
CCEEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCC
KGAISKANEIVSLNTKRYLLLKQFENPANPEIHQKTTGPEIWKDTKGNIDILISGVGTGG
CHHHHCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCEEEEEEECCCCC
TITGITKYIKIKEGKKDLISVAVEPLESPVITQFLSGKKIKPGLHKIQGIGAGFIPKNLD
CHHCEEEEEEEECCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCC
LSLIDRIITVSSEEAIFNAKKIMKKEGILSGISSGAAIAAALKIQKENDFKNKNIVVILP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCEEEEEC
SSGERYLSTELFSRSFKNENNY
CCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438