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The map label for this gene is ptsH

Identifier: 21672355

GI number: 21672355

Start: 70962

End: 71219

Strand: Reverse

Name: ptsH

Synonym: BUsg062

Alternate gene names: 21672355

Gene position: 71219-70962 (Counterclockwise)

Preceding gene: 21672356

Following gene: 21672354

Centisome position: 11.1

GC content: 27.91

Gene sequence:

>258_bases
ATGTTTCAAAAAGAAATTAAAATCAATGCATTACATGGTTTACATACTCGACCTGCAGCTGAATTTGTAAAGGAAGCAAA
AAATTTTATCTCCGATATTCATATTATTTACCATGGAAAATCTGTTAACGCAAAAAGCTTATTTAAAATTCAAACTTTAG
GTTTAGTGAAAGATAGCGTTATTATATTGTCAGCAGAAGGTGAAGATGAAAAAAAAGCTGTTGAACATTTATCTAAAATA
ATGACAGAACTAGAATAA

Upstream 100 bases:

>100_bases
TTTTTATTATAAAAGTTGTATTTTAATATTTTAAATTAAAATTTATTTTAAAATATATTAACAATTATTCTATAAACTTA
ATATTATATAAGGAAAAAAA

Downstream 100 bases:

>100_bases
ATTATAATTGAAAATAATTTTTTTTATAAAAAATACCGGAGGTTTTACATTACTTCCGGATTTATTTTATAATAAATAAA
ATATATTCGCCTGACATTCT

Product: phosphocarrier protein Hpr

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; D-glucosamine-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; galactitol-1-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm]; fructose-1-phosphate [Cytoplasm]; glucose-6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm]; salicin-6-phosphate [Cytoplasm]; arbutin-6-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein

Number of amino acids: Translated: 85; Mature: 85

Protein sequence:

>85_residues
MFQKEIKINALHGLHTRPAAEFVKEAKNFISDIHIIYHGKSVNAKSLFKIQTLGLVKDSVIILSAEGEDEKKAVEHLSKI
MTELE

Sequences:

>Translated_85_residues
MFQKEIKINALHGLHTRPAAEFVKEAKNFISDIHIIYHGKSVNAKSLFKIQTLGLVKDSVIILSAEGEDEKKAVEHLSKI
MTELE
>Mature_85_residues
MFQKEIKINALHGLHTRPAAEFVKEAKNFISDIHIIYHGKSVNAKSLFKIQTLGLVKDSVIILSAEGEDEKKAVEHLSKI
MTELE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain

Homologues:

Organism=Escherichia coli, GI1788755, Length=85, Percent_Identity=65.8823529411765, Blast_Score=122, Evalue=5e-30,

Paralogues:

None

Copy number: 4180 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 2100 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 3235 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PTHP_BUCAP (Q8KA49)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660422.1
- ProteinModelPortal:   Q8KA49
- SMR:   Q8KA49
- EnsemblBacteria:   EBBUCT00000000114
- GeneID:   1005878
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg062
- GeneTree:   EBGT00050000008028
- HOGENOM:   HBG653254
- OMA:   IVECNGK
- ProtClustDB:   CLSK315127
- BioCyc:   BAPH198804:BUSG062-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- Gene3D:   G3DSA:3.30.1340.10
- PRINTS:   PR00107
- TIGRFAMs:   TIGR01003

Pfam domain/function: PF00381 PTS-HPr; SSF55594 HPr_protein

EC number: NA

Molecular weight: Translated: 9571; Mature: 9571

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS51350 PTS_HPR_DOM; PS00369 PTS_HPR_HIS; PS00589 PTS_HPR_SER

Important sites: ACT_SITE 15-15

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQKEIKINALHGLHTRPAAEFVKEAKNFISDIHIIYHGKSVNAKSLFKIQTLGLVKDSV
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCCCCE
IILSAEGEDEKKAVEHLSKIMTELE
EEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFQKEIKINALHGLHTRPAAEFVKEAKNFISDIHIIYHGKSVNAKSLFKIQTLGLVKDSV
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHCCCCCCE
IILSAEGEDEKKAVEHLSKIMTELE
EEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sorbitol [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm]; mannitol [Periplasm]; glucosamine [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; galactitol [Periplasm]; fructose [Periplasm]; beta-D-glucose [Periplasm]; diacetylchitobiose [Periplasm]; cellobiose [Periplasm]; salicin [Periplasm]; arbutin [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + mannitol [Periplasm] = mannitol-1-phosp

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438