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The map label for this gene is dnaG

Identifier: 21672346

GI number: 21672346

Start: 61015

End: 62754

Strand: Reverse

Name: dnaG

Synonym: BUsg053

Alternate gene names: 21672346

Gene position: 62754-61015 (Counterclockwise)

Preceding gene: 21672347

Following gene: 21672345

Centisome position: 9.78

GC content: 23.56

Gene sequence:

>1740_bases
ATGTCTGGAAAAATACCTAAATATTTCATTAATGAACTATTATTTCGAACTAATATCGTTGAACTTATAGAGACACGAAT
AAAGTTAAAAAAAAATGGTAAAAATTATCAAACTCATTGTCCTTTCCACAATGATAAAACTCCATCCTTTACTGTAAGTT
ACGAAAAACAATTTTATTATTGTTTTGGATGCAGTGCGCATGGCAATGCAATTGATTTTTTAATCAACTACGACAATTTA
AATTTTGTAGAAAGTATTGAAGAACTTGCTACAATTCATAGTATTGAGATTCCATTTGATAAAAAAGGTACAATTGAAAA
AAAAATCTATCTAAAAAAACAAAAATTATACTTTTTAATGGATAAAATGTCTAAATTATATCAAAAAAATATTATTTTTA
CTAATTCAGCAAACAAATATTTAACTGAAAGAGGAATTAATAAAAATATGATTGATTTTTTTTGTATTGGATTTTCAAGT
AATAAATGGGATTTATTTTCTAAACAATTCAATATAAAAAAAGATTTTGAGAAAGATTTATTACATTATAAAATAATTTC
TACTAATAAAAACGGATATATATATGATACTTTTCAAGGACGTATCGTTTTTCCTATACATGATCAACATGGAAGAATTA
TAAGTTTTGGAGGACGTTCAATAACAAATAATATTTTGCCAAAATATCTTAATTCACATGAAAATGATATTTTCCATAAA
GGAAAACAAATTTATGGATTATATCAAGTTAAAAAAAAACATTCCAAACCAAAATATCTACTAGTTGTAGAAGGATATAT
AGATGTTATCATGTTAACACAATATAACATTGAATATGTAGTGTCATCATTAGGCACATCTATAACAAAAGAACACATAC
AAATACTTTTTCGTCATACTAATACAGTCATATATTGTTATGATGGTGATGATGCAGGAAGAAATGCCTCTTGGAGGACT
TTAAAAATTGCATTACCATATATATCTGACAAAAAAAATATAAAATTTATAATATTACCTAATAATGAAGATCCTGATAC
AATTATAAAAAAAGAAGGCCAAAAAAAATTTCAATTACGAATTGACAACGCTCTTACTATGTCAAAATTTTTTTTTAAAA
ATATTTTAAAAAACGTTGACTTGTCATCTGATGATGATAAGTTTCACCTTAGTGTACGTGCTTTACCTTTAATAAATTGC
ATATCTAGTGATACAATAAGGATTTACTTGAGACAAATATTAGCCAGAACAATAGGGATTGTAGATGACTATCAATTTGA
AAAATTTTTATACGAAAAAGAAAGAAAAAAGAAAAGTTTACAGTTTCAAATTAAACATACTCCTATGCGAACTCTTATAG
GTTTACTTATTCAAAATCCTAATTTAGCAAGATTAGTACCTTCAACAAAAAAATTTGAAAATGAAAAAATACAAGGACTT
CCTATTTTTTTAGAAATTTTAAAAACATGTTCTGATAATCCCAATTTTAATACAGGTCAATTATTAGAGTTTTACAGATA
TAGTAAAATAATTAATATTTTAAAAATTTTATCTAAATGGGATCATATGATAATTAAAGAAAAAATTCAAAATATGTTTT
TAGATTTATTAAAAAATACATATAGTAAAATTCTAGAAAAAAGACAAGAAAAATTAATTGCAAAAGAAAGAATTCAAGGA
TTAACAATAGACGAAAAAAAAGAGATCTGGTCTATTAATAAAAAGCTATCAAAAAAATAG

Upstream 100 bases:

>100_bases
AAGACGTATTCGTATGTACTAAGATATTTCTTAAAAATAAAAAAATTAAAAAACCGTTCTTTACTTAAGAACGGAATTTT
ATTCTACCATATAAAAATAT

Downstream 100 bases:

>100_bases
TTTTTTTATATAAAAAGAATTTTAAATATCAATTAAAAAATAAATTATTATTCATTTATTGACTAAAAAGTCAATTAATA
TTCATTATATCAACAAAATT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MSGKIPKYFINELLFRTNIVELIETRIKLKKNGKNYQTHCPFHNDKTPSFTVSYEKQFYYCFGCSAHGNAIDFLINYDNL
NFVESIEELATIHSIEIPFDKKGTIEKKIYLKKQKLYFLMDKMSKLYQKNIIFTNSANKYLTERGINKNMIDFFCIGFSS
NKWDLFSKQFNIKKDFEKDLLHYKIISTNKNGYIYDTFQGRIVFPIHDQHGRIISFGGRSITNNILPKYLNSHENDIFHK
GKQIYGLYQVKKKHSKPKYLLVVEGYIDVIMLTQYNIEYVVSSLGTSITKEHIQILFRHTNTVIYCYDGDDAGRNASWRT
LKIALPYISDKKNIKFIILPNNEDPDTIIKKEGQKKFQLRIDNALTMSKFFFKNILKNVDLSSDDDKFHLSVRALPLINC
ISSDTIRIYLRQILARTIGIVDDYQFEKFLYEKERKKKSLQFQIKHTPMRTLIGLLIQNPNLARLVPSTKKFENEKIQGL
PIFLEILKTCSDNPNFNTGQLLEFYRYSKIINILKILSKWDHMIIKEKIQNMFLDLLKNTYSKILEKRQEKLIAKERIQG
LTIDEKKEIWSINKKLSKK

Sequences:

>Translated_579_residues
MSGKIPKYFINELLFRTNIVELIETRIKLKKNGKNYQTHCPFHNDKTPSFTVSYEKQFYYCFGCSAHGNAIDFLINYDNL
NFVESIEELATIHSIEIPFDKKGTIEKKIYLKKQKLYFLMDKMSKLYQKNIIFTNSANKYLTERGINKNMIDFFCIGFSS
NKWDLFSKQFNIKKDFEKDLLHYKIISTNKNGYIYDTFQGRIVFPIHDQHGRIISFGGRSITNNILPKYLNSHENDIFHK
GKQIYGLYQVKKKHSKPKYLLVVEGYIDVIMLTQYNIEYVVSSLGTSITKEHIQILFRHTNTVIYCYDGDDAGRNASWRT
LKIALPYISDKKNIKFIILPNNEDPDTIIKKEGQKKFQLRIDNALTMSKFFFKNILKNVDLSSDDDKFHLSVRALPLINC
ISSDTIRIYLRQILARTIGIVDDYQFEKFLYEKERKKKSLQFQIKHTPMRTLIGLLIQNPNLARLVPSTKKFENEKIQGL
PIFLEILKTCSDNPNFNTGQLLEFYRYSKIINILKILSKWDHMIIKEKIQNMFLDLLKNTYSKILEKRQEKLIAKERIQG
LTIDEKKEIWSINKKLSKK
>Mature_578_residues
SGKIPKYFINELLFRTNIVELIETRIKLKKNGKNYQTHCPFHNDKTPSFTVSYEKQFYYCFGCSAHGNAIDFLINYDNLN
FVESIEELATIHSIEIPFDKKGTIEKKIYLKKQKLYFLMDKMSKLYQKNIIFTNSANKYLTERGINKNMIDFFCIGFSSN
KWDLFSKQFNIKKDFEKDLLHYKIISTNKNGYIYDTFQGRIVFPIHDQHGRIISFGGRSITNNILPKYLNSHENDIFHKG
KQIYGLYQVKKKHSKPKYLLVVEGYIDVIMLTQYNIEYVVSSLGTSITKEHIQILFRHTNTVIYCYDGDDAGRNASWRTL
KIALPYISDKKNIKFIILPNNEDPDTIIKKEGQKKFQLRIDNALTMSKFFFKNILKNVDLSSDDDKFHLSVRALPLINCI
SSDTIRIYLRQILARTIGIVDDYQFEKFLYEKERKKKSLQFQIKHTPMRTLIGLLIQNPNLARLVPSTKKFENEKIQGLP
IFLEILKTCSDNPNFNTGQLLEFYRYSKIINILKILSKWDHMIIKEKIQNMFLDLLKNTYSKILEKRQEKLIAKERIQGL
TIDEKKEIWSINKKLSKK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=582, Percent_Identity=48.7972508591065, Blast_Score=620, Evalue=1e-179,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_BUCAP (P32000)

Other databases:

- EMBL:   AE013218
- EMBL:   M90644
- PIR:   PC1137
- RefSeq:   NP_660413.1
- ProteinModelPortal:   P32000
- EnsemblBacteria:   EBBUCT00000000540
- GeneID:   1005869
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg053
- GeneTree:   EBGT00050000007723
- HOGENOM:   HBG573408
- OMA:   ALAEKEW
- ProtClustDB:   CLSK315071
- BioCyc:   BAPH198804:BUSG053-MONOMER
- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:1.10.860.10
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00766
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 68271; Mature: 68139

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKIPKYFINELLFRTNIVELIETRIKLKKNGKNYQTHCPFHNDKTPSFTVSYEKQFYY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEECEEEE
CFGCSAHGNAIDFLINYDNLNFVESIEELATIHSIEIPFDKKGTIEKKIYLKKQKLYFLM
EEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHEEECCCCCCCCCCHHHEEHHHHHHHHH
DKMSKLYQKNIIFTNSANKYLTERGINKNMIDFFCIGFSSNKWDLFSKQFNIKKDFEKDL
HHHHHHHHCCEEEECCCCHHHHHCCCCCCCEEEEEEECCCCCCCHHHHCCCCHHHHHHCC
LHYKIISTNKNGYIYDTFQGRIVFPIHDQHGRIISFGGRSITNNILPKYLNSHENDIFHK
EEEEEEEECCCCEEEEECCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHC
GKQIYGLYQVKKKHSKPKYLLVVEGYIDVIMLTQYNIEYVVSSLGTSITKEHIQILFRHT
CCHHEEHHHHHHHCCCCCEEEEEECCEEEEEEEECCHHHHHHHHCCHHHHHHHHHHEEEC
NTVIYCYDGDDAGRNASWRTLKIALPYISDKKNIKFIILPNNEDPDTIIKKEGQKKFQLR
CEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHCCCCEEEEEE
IDNALTMSKFFFKNILKNVDLSSDDDKFHLSVRALPLINCISSDTIRIYLRQILARTIGI
ECCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEHHHHHCCCCHHHHHHHHHHHHHHCC
VDDYQFEKFLYEKERKKKSLQFQIKHTPMRTLIGLLIQNPNLARLVPSTKKFENEKIQGL
CCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCHHHCCCCCCCCC
PIFLEILKTCSDNPNFNTGQLLEFYRYSKIINILKILSKWDHMIIKEKIQNMFLDLLKNT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSKILEKRQEKLIAKERIQGLTIDEKKEIWSINKKLSKK
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGKIPKYFINELLFRTNIVELIETRIKLKKNGKNYQTHCPFHNDKTPSFTVSYEKQFYY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEECEEEE
CFGCSAHGNAIDFLINYDNLNFVESIEELATIHSIEIPFDKKGTIEKKIYLKKQKLYFLM
EEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHEEECCCCCCCCCCHHHEEHHHHHHHHH
DKMSKLYQKNIIFTNSANKYLTERGINKNMIDFFCIGFSSNKWDLFSKQFNIKKDFEKDL
HHHHHHHHCCEEEECCCCHHHHHCCCCCCCEEEEEEECCCCCCCHHHHCCCCHHHHHHCC
LHYKIISTNKNGYIYDTFQGRIVFPIHDQHGRIISFGGRSITNNILPKYLNSHENDIFHK
EEEEEEEECCCCEEEEECCCEEEEEEECCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHC
GKQIYGLYQVKKKHSKPKYLLVVEGYIDVIMLTQYNIEYVVSSLGTSITKEHIQILFRHT
CCHHEEHHHHHHHCCCCCEEEEEECCEEEEEEEECCHHHHHHHHCCHHHHHHHHHHEEEC
NTVIYCYDGDDAGRNASWRTLKIALPYISDKKNIKFIILPNNEDPDTIIKKEGQKKFQLR
CEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHCCCCEEEEEE
IDNALTMSKFFFKNILKNVDLSSDDDKFHLSVRALPLINCISSDTIRIYLRQILARTIGI
ECCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEHHHHHCCCCHHHHHHHHHHHHHHCC
VDDYQFEKFLYEKERKKKSLQFQIKHTPMRTLIGLLIQNPNLARLVPSTKKFENEKIQGL
CCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCHHHCCCCCCCCC
PIFLEILKTCSDNPNFNTGQLLEFYRYSKIINILKILSKWDHMIIKEKIQNMFLDLLKNT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSKILEKRQEKLIAKERIQGLTIDEKKEIWSINKKLSKK
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438; 1398077