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The map label for this gene is spo0A

Identifier: 30264247

GI number: 30264247

Start: 4008951

End: 4009745

Strand: Reverse

Name: spo0A

Synonym: BA_4394

Alternate gene names: 30264247

Gene position: 4009745-4008951 (Counterclockwise)

Preceding gene: 30264248

Following gene: 30264246

Centisome position: 76.71

GC content: 37.36

Gene sequence:

>795_bases
GTGGAGAAAATTAAAGTATGTCTTGTGGATGATAATAAAGAATTAGTATCAATGCTAGAGAGTTATGTAGCTGCTCAAGA
TGATATGGAAGTAATCGGTACTGCTTATAATGGTCAAGAGTGTTTAAATTTATTAAAAGATAAGCAGCCGGATGTACTCG
TATTGGATATTATTATGCCACATTTAGATGGTTTAGCTGTACTAGAGAAAATGCGACATATTGAAAGGTTAAGACAGCCT
AGCGTAATTATGTTGACAGCATTTGGACAAGAAGATGTGACGAAAAAAGCAGTTGACTTAGGTGCTTCATATTTCATATT
AAAACCATTTGATATGGAAAATTTAACGAGTCATATTCGTCAAGTGAGCGGTAAGGCAAACGCTACTATTAAACGTCCAC
TGCCATCTTTCCGATCAGCAACAACAGTAGATGGAAAACCGAAAAATTTAGACGCGAGCATTACGAGTATCATTCATGAA
ATTGGTGTGCCTGCTCATATTAAAGGGTACATGTACTTAAGGGAAGCAATTTCTATGGTGTACAATGACATCGAATTACT
TGGATCTATTACGAAAGTATTGTATCCAGATATCGCGAAGAAATATAATACAACAGCAAGCCGCGTTGAGCGTGCAATCC
GTCACGCAATTGAAGTAGCATGGAGTCGTGGGAATATTGATTCTATTTCGTCCTTATTCGGTTATACAGTATCCATGTCA
AAAGCAAAACCTACGAACTCCGAGTTTATTGCAATGGTTGCGGATAAGCTTAGACTTGAACATAAGGCTAGCTGA

Upstream 100 bases:

>100_bases
ACAAACTTTAAAAGGGAATTTTCACACAATTGTCGAACAATTCATGTAGCCTAGAAGGATACACATGTCGGAGAATCGAT
TCGGTAAGGGAGGAAAAGCT

Downstream 100 bases:

>100_bases
GAATAGAAGAAGTAAGAGATTTAAGTTTTCATAAAGCTTAATCATTCTTGAAATTTATACTTGATAGTACATTTAAAACT
AAAGAACTTTTTATGTAATG

Product: stage 0 sporulation protein A

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKDKQPDVLVLDIIMPHLDGLAVLEKMRHIERLRQP
SVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIRQVSGKANATIKRPLPSFRSATTVDGKPKNLDASITSIIHE
IGVPAHIKGYMYLREAISMVYNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVSMS
KAKPTNSEFIAMVADKLRLEHKAS

Sequences:

>Translated_264_residues
MEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKDKQPDVLVLDIIMPHLDGLAVLEKMRHIERLRQP
SVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIRQVSGKANATIKRPLPSFRSATTVDGKPKNLDASITSIIHE
IGVPAHIKGYMYLREAISMVYNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVSMS
KAKPTNSEFIAMVADKLRLEHKAS
>Mature_264_residues
MEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKDKQPDVLVLDIIMPHLDGLAVLEKMRHIERLRQP
SVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIRQVSGKANATIKRPLPSFRSATTVDGKPKNLDASITSIIHE
IGVPAHIKGYMYLREAISMVYNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVSMS
KAKPTNSEFIAMVADKLRLEHKAS

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=112, Percent_Identity=41.0714285714286, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788550, Length=112, Percent_Identity=36.6071428571429, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790299, Length=164, Percent_Identity=31.7073170731707, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082052, Length=130, Percent_Identity=31.5384615384615, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SP0A_BACAN (P52928)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- EMBL:   U09970
- PIR:   S60868
- RefSeq:   NP_846624.1
- RefSeq:   YP_021039.2
- RefSeq:   YP_030326.1
- ProteinModelPortal:   P52928
- EnsemblBacteria:   EBBACT00000009270
- EnsemblBacteria:   EBBACT00000018747
- EnsemblBacteria:   EBBACT00000019258
- GeneID:   1087648
- GeneID:   2814788
- GeneID:   2852644
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_4394
- KEGG:   bar:GBAA_4394
- KEGG:   bat:BAS4076
- TIGR:   BA_4394
- TIGR:   GBAA_4394
- GeneTree:   EBGT00070000031787
- HOGENOM:   HBG304664
- OMA:   AHIKGYL
- ProtClustDB:   CLSK873420
- BioCyc:   BANT260799:BAS4076-MONOMER
- BioCyc:   BANT261594:GBAA4394-MONOMER
- GO:   GO:0005737
- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PIRSF:   PIRSF002937
- ProDom:   PD010908
- SMART:   SM00448
- TIGRFAMs:   TIGR02875

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C; SSF46894 Bipartite_resp_reg_C-effector; SSF52172 CheY_like

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 29441; Mature: 29441

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKDKQPDVLVLDIIMP
CCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCCEEEHHHHHH
HLDGLAVLEKMRHIERLRQPSVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIR
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
QVSGKANATIKRPLPSFRSATTVDGKPKNLDASITSIIHEIGVPAHIKGYMYLREAISMV
HHCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVSMS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECC
KAKPTNSEFIAMVADKLRLEHKAS
CCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKIKVCLVDDNKELVSMLESYVAAQDDMEVIGTAYNGQECLNLLKDKQPDVLVLDIIMP
CCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCCEEEHHHHHH
HLDGLAVLEKMRHIERLRQPSVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLTSHIR
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
QVSGKANATIKRPLPSFRSATTVDGKPKNLDASITSIIHEIGVPAHIKGYMYLREAISMV
HHCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YNDIELLGSITKVLYPDIAKKYNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVSMS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECC
KAKPTNSEFIAMVADKLRLEHKAS
CCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629; 7885226