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The map label for this gene is rasP [H]

Identifier: 30263824

GI number: 30263824

Start: 3635626

End: 3636888

Strand: Reverse

Name: rasP [H]

Synonym: BA_3958

Alternate gene names: 30263824

Gene position: 3636888-3635626 (Counterclockwise)

Preceding gene: 30263825

Following gene: 30263823

Centisome position: 69.57

GC content: 36.74

Gene sequence:

>1263_bases
ATGGAATTGAATACAGCGATTGCCTTTATATTAATTTTCGGTGCACTCGTATTTTTCCATGAGCTAGGGCATCTATATTT
CGCAAAAAGAGCAGGTATTTTATGCCGCGAGTTTGCGATTGGTTTTGGTCCGAAAATATTCTCATTTGAAAAGAATGAAA
CGGTGTATACGATTCGATTACTGCCCCTTGGTGGCTATGTAAGAATGGCTGGCGAGGATGCAGACACAGTTGAGTTAAAG
CCTGGGAAAAAGGTTGGCCTTGTATTAAATGCAAAAGACGAAGTTGCAAAATTAGTTTTTGATGGATATGAAAAGTATCC
CAATGTTCGCGTTATCGAAGTCGAACAAGCTGATTTAGAGCATAATTTAACAATTTCGGGTTATGAAGAGTACGAGGAAG
AGCTTCAAACATTCCGAGTGAACGAGAAGGCTCGTATTATTACTGCCGGTGAAGAAATTCAAATTGCTCCGTATAATAGG
CAGTTTGGCTCTAAGAAATTAGGACAACGTGCGTTAACAATCTTTGCGGGCCCTGCAATGAACTTCATTTTAGCATTTGT
TATTTTTGTGATTCTTGGATTTGTACAAGGTGTTCCTGTTGACAAACCAATGGTCGGAAAAGTGATGGAGAATAGTGCAG
CAGAGCAAGCTGGATTAAAAGAAAATGATACAATTCAAGCTATTGATGGGAAAAACACAAGTACATGGAAAGATGTTGTT
ACCATTGTACGTGAAAATCCAAATAAAGAAATTACGTTACAAGTAAAGCGTGATAGTGAACAGTTTAATGTGAAAGTAAC
GCCAACGCTTGATAAAGAAGGTAAAGAAGAAGTTGGTAGAATTGGTGTTTACTCTCCTGTAGAGAAAACAGTGATGGGTT
CTATTAAATCAGGTTTTGAACAAACATACCAATGGACGAAACTAATTTTTGAGTCTTTTGTGAAATTAGTAACTGGTCAA
TTCTCTATTAATGAGTTGTCAGGTCCAGTAGGAATTTATAATCTAACAGATCAAGTTGTAGATTATGGATTTACGCGTGT
TTTAAGTTTAGCAGCCGTTTTAAGTATTAACCTTGGTTTATTTAATTTACTACCAGTTCCTGCTTTAGACGGTGGACGTT
TGTTCTTCTTCTTAATTGAGGCATTGCGTGGGAAACCAATTGATCGCCAGAAAGAAGGAATGGTTCACTTTATTGGTTTT
GCATTATTAATGTTACTTATGTTAGTTGTAACTTGGAATGACATTCGTAAGTTTTTCTTGTAA

Upstream 100 bases:

>100_bases
AACCATCACAATGTCATTGCACGTCCGAGCTTAGAGGAAATTCTGGAAATTGATGCAGCCACAAGACGGTTTGTGATGGA
ACAAATTTAGCAAAGGTGGT

Downstream 100 bases:

>100_bases
AATAAAGTGAAACTTATAATGAAATAGAGCTCGTAGAATAAAATCCCTCACCTAACTCGAGGTGAGGGGTTTTCCTGCGT
GTAAGAGTAAAATGAAGAGG

Product: membrane-associated zinc metalloprotease, putative

Products: NA

Alternate protein names: Regulating alternative sigma factor protease; Regulating anti-sigma-W factor activity protease [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MELNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTIRLLPLGGYVRMAGEDADTVELK
PGKKVGLVLNAKDEVAKLVFDGYEKYPNVRVIEVEQADLEHNLTISGYEEYEEELQTFRVNEKARIITAGEEIQIAPYNR
QFGSKKLGQRALTIFAGPAMNFILAFVIFVILGFVQGVPVDKPMVGKVMENSAAEQAGLKENDTIQAIDGKNTSTWKDVV
TIVRENPNKEITLQVKRDSEQFNVKVTPTLDKEGKEEVGRIGVYSPVEKTVMGSIKSGFEQTYQWTKLIFESFVKLVTGQ
FSINELSGPVGIYNLTDQVVDYGFTRVLSLAAVLSINLGLFNLLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGF
ALLMLLMLVVTWNDIRKFFL

Sequences:

>Translated_420_residues
MELNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTIRLLPLGGYVRMAGEDADTVELK
PGKKVGLVLNAKDEVAKLVFDGYEKYPNVRVIEVEQADLEHNLTISGYEEYEEELQTFRVNEKARIITAGEEIQIAPYNR
QFGSKKLGQRALTIFAGPAMNFILAFVIFVILGFVQGVPVDKPMVGKVMENSAAEQAGLKENDTIQAIDGKNTSTWKDVV
TIVRENPNKEITLQVKRDSEQFNVKVTPTLDKEGKEEVGRIGVYSPVEKTVMGSIKSGFEQTYQWTKLIFESFVKLVTGQ
FSINELSGPVGIYNLTDQVVDYGFTRVLSLAAVLSINLGLFNLLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGF
ALLMLLMLVVTWNDIRKFFL
>Mature_420_residues
MELNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTIRLLPLGGYVRMAGEDADTVELK
PGKKVGLVLNAKDEVAKLVFDGYEKYPNVRVIEVEQADLEHNLTISGYEEYEEELQTFRVNEKARIITAGEEIQIAPYNR
QFGSKKLGQRALTIFAGPAMNFILAFVIFVILGFVQGVPVDKPMVGKVMENSAAEQAGLKENDTIQAIDGKNTSTWKDVV
TIVRENPNKEITLQVKRDSEQFNVKVTPTLDKEGKEEVGRIGVYSPVEKTVMGSIKSGFEQTYQWTKLIFESFVKLVTGQ
FSINELSGPVGIYNLTDQVVDYGFTRVLSLAAVLSINLGLFNLLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGF
ALLMLLMLVVTWNDIRKFFL

Specific function: Is responsible for Site-2 cleavage of the RsiW anti- sigma factor. This results, after a third proteolytic step catalyzed by the ClpXP protease, in the release of SigW and the transcription activation of the genes under the control of the sigma-W factor [

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=448, Percent_Identity=29.4642857142857, Blast_Score=164, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 47078; Mature: 47078

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTIRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCEEEEEEE
LPLGGYVRMAGEDADTVELKPGKKVGLVLNAKDEVAKLVFDGYEKYPNVRVIEVEQADLE
EECCCEEEECCCCCCEEEECCCCEEEEEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCC
HNLTISGYEEYEEELQTFRVNEKARIITAGEEIQIAPYNRQFGSKKLGQRALTIFAGPAM
CCCEECCHHHHHHHHHHEECCCCEEEEECCCEEEEECCCCCCCHHHHHHHEEEEEECHHH
NFILAFVIFVILGFVQGVPVDKPMVGKVMENSAAEQAGLKENDTIQAIDGKNTSTWKDVV
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHH
TIVRENPNKEITLQVKRDSEQFNVKVTPTLDKEGKEEVGRIGVYSPVEKTVMGSIKSGFE
HHHHCCCCCEEEEEEEECCCEEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHH
QTYQWTKLIFESFVKLVTGQFSINELSGPVGIYNLTDQVVDYGFTRVLSLAAVLSINLGL
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCH
FNLLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGFALLMLLMLVVTWNDIRKFFL
HHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELNTAIAFILIFGALVFFHELGHLYFAKRAGILCREFAIGFGPKIFSFEKNETVYTIRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCEEEEEEE
LPLGGYVRMAGEDADTVELKPGKKVGLVLNAKDEVAKLVFDGYEKYPNVRVIEVEQADLE
EECCCEEEECCCCCCEEEECCCCEEEEEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCC
HNLTISGYEEYEEELQTFRVNEKARIITAGEEIQIAPYNRQFGSKKLGQRALTIFAGPAM
CCCEECCHHHHHHHHHHEECCCCEEEEECCCEEEEECCCCCCCHHHHHHHEEEEEECHHH
NFILAFVIFVILGFVQGVPVDKPMVGKVMENSAAEQAGLKENDTIQAIDGKNTSTWKDVV
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHH
TIVRENPNKEITLQVKRDSEQFNVKVTPTLDKEGKEEVGRIGVYSPVEKTVMGSIKSGFE
HHHHCCCCCEEEEEEEECCCEEEEEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHH
QTYQWTKLIFESFVKLVTGQFSINELSGPVGIYNLTDQVVDYGFTRVLSLAAVLSINLGL
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCH
FNLLPVPALDGGRLFFFLIEALRGKPIDRQKEGMVHFIGFALLMLLMLVVTWNDIRKFFL
HHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]