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The map label for this gene is miaB [H]

Identifier: 30263778

GI number: 30263778

Start: 3581075

End: 3582604

Strand: Reverse

Name: miaB [H]

Synonym: BA_3908

Alternate gene names: 30263778

Gene position: 3582604-3581075 (Counterclockwise)

Preceding gene: 30263779

Following gene: 30263777

Centisome position: 68.54

GC content: 36.67

Gene sequence:

>1530_bases
ATGAACGAGCAACAACGATTAGCAAGTCAACAAGTGAATTCCTCTACGAAAAAAGAAGAGAAAGATTATAGCAAATACTT
TGAAAGTGTATACCAACCACCTTCCTTAAAAGACGCGAAAAAACGAGGGAAAGAAGAAGTTAAAATTGAACGGGATTTTG
GCTTGCCTGAAGAGTTTCGCAATTTTGGTACAGGAAGAAAGTTTTATATTCGTACATACGGTTGTCAAATGAATGAGCAT
GATACAGAAGTAATGGCGGGTATTTTCACTGCACTGGGATATGAACCAACCTTTTCAACTGAAGACGCAGATGTAGTATT
ATTAAATACTTGTGCCATTCGTGAAAATGCTGAAAATAAAGTGTTCGGTGAACTAGGTCATTTAAAGTCACTAAAACGAA
GAAATTCGGACCTTTTAATTGGTGTATGTGGTTGTATGTCTCAAGAGGAATCTGTTGTAAATAAAATTATGCAAAAAAAT
CAGCATGTAGATATGGTGTTTGGAACGCATAATATTCATAGATTACCGTACATTCTAAAAGATGCAATGTTCTCGAAGGA
GACGGTAGTTGAAGTTTGGTCCAAAGAAGGAGACGTAATTGAAAACCTTCCGAAAGTACGCCGTGGTGATATTAAAGCTT
GGGTAAATATTATGTATGGCTGTGATAAGTTCTGTACATATTGTATCGTACCGTATACACGCGGAAAAGAGCGAAGCAGA
CGTCCAGAAGACATTATCCAAGAGATTCGTCATTTAGCTGCGAATGGTTATAAAGAAATTACGTTACTTGGACAGAACGT
AAATGCATATGGTAAAGACTTTGAAGATATAGAATACGGTCTTGGCGATTTAATGGACGAACTCCGTAAAGTTGATATTG
CCCGTATTCGTTTTACAACGAGCCATCCACGCGATTTCGATGATCACTTAATTGAAGTGCTTGGAAAAGGCGGAAACTTA
GTAGAACATATTCACTTACCAGTTCAATCTGGAAGCACAGAAATGTTAAAAATTATGGCGCGTAAATATTCACGTGAGCA
TTATTTAGAGCTTGTAAGAAAAATTAAAGAAGCAATTCCAAACGCGGTATTAACAACTGATATTATCGTCGGTTTCCCGA
ATGAAACAGATGAGCAATTTGAAGAAACGATGTCGTTGTATCGTGAAGTAGGATTTGATACTGCCTTTACCTTTATTTAT
TCTCCGCGTGAAGGTACACCAGCTGCAAAAATGAAAGACAATGTACCGATGGAAGTAAAGAAAGAACGTCTTCAACGCTT
AAATGCATTAGTAAATAAATTGGCAATTGAAAAGAATGATCGTTATAAAGGTCAAATTGTAGAAGTGTTAGTTGATGGGG
AAAGTAAAAATAATCCAGAAGTACTTGCAGGTTATACTCGTACGAATAAACTTGTAAACTTCGTTGCTCCAAAGTCTTTA
ATTGGTCAGCTTGTGAAAATAAAAGTAACGGATGCAAAAACTTGGTCTCTTAACGGAGAATTAGTTGAAGAGCCGATTGA
GGTGGAATAA

Upstream 100 bases:

>100_bases
ACAAGTATCTCTCAAGCTATCTAACCATTTTTGAGGTGGGTCTAAGCGCATTAAAATGGACTATAGAGTGTGTATAGTTA
AGGGTGAAAGGAGATTACCG

Downstream 100 bases:

>100_bases
TAGATGAAAGTATATTCGAAAGATGAAATTGTTGAGCAAGCGAAAGAATTAGCAAAAATGATTTCTGAAACAGAAGAAGT
TGATTTCTTTAAGAAAGCAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKSLKRRNSDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
IGQLVKIKVTDAKTWSLNGELVEEPIEVE

Sequences:

>Translated_509_residues
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKSLKRRNSDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
IGQLVKIKVTDAKTWSLNGELVEEPIEVE
>Mature_509_residues
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKSLKRRNSDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
IGQLVKIKVTDAKTWSLNGELVEEPIEVE

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=483, Percent_Identity=36.8530020703934, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI28872784, Length=483, Percent_Identity=36.8530020703934, Blast_Score=302, Evalue=5e-82,
Organism=Homo sapiens, GI93277076, Length=455, Percent_Identity=28.7912087912088, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=44.5454545454545, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1787057, Length=454, Percent_Identity=27.0925110132159, Blast_Score=155, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=480, Percent_Identity=35.625, Blast_Score=273, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI71996771, Length=307, Percent_Identity=30.9446254071661, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI21356207, Length=549, Percent_Identity=34.0619307832423, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI19922432, Length=457, Percent_Identity=30.8533916849015, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 58348; Mature: 58348

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEEEECCCCCCHHHH
NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENK
HCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCCH
VFGELGHLKSLKRRNSDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHCHHHHHHHHCCCCCHHHHCHHHHHCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEEC
SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCC
NAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCHHH
IGQLVKIKVTDAKTWSLNGELVEEPIEVE
HCCEEEEEEECCEEEECCCHHHCCCCCCC
>Mature Secondary Structure
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKDAKKRGKEEVKIERDFGLPEEFR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHEEEECCCCCCHHHH
NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENK
HCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCCH
VFGELGHLKSLKRRNSDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHCHHHHHHHHCCCCCHHHHCHHHHHCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEEC
SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCC
NAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCHHH
IGQLVKIKVTDAKTWSLNGELVEEPIEVE
HCCEEEEEEECCEEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA