BacMap

Definition	Bacillus anthracis str. Ames, complete genome.
Accession	NC_003997
Length	5,227,293

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The map label for this gene is yngF [H]

Identifier: 30263476

GI number: 30263476

Start: 3287450

End: 3288241

Strand: Reverse

Name: yngF [H]

Synonym: BA_3583

Alternate gene names: 30263476

Gene position: 3288241-3287450 (Counterclockwise)

Preceding gene: 30263477

Following gene: 30263475

Centisome position: 62.91

GC content: 36.87

Gene sequence:

>792_bases
ATGAAAAATGAACGATTAGTAATATGCAGTAAGAAAGGGAGTAGTGCAGTCATTACAATTCAAAACCCACCAGTCAACGC
TTTAAGTTTAGAAGTAGTGCAACAATTAATAAATGTATTAGAGGAAATAGAGATGGACGATGATATTGCAGTCGTAATTA
TAACTGGAATTGGGGGGAAAGCCTTTGTAGCTGGAGGGGATATAAAAGAATTTCCAGGTTGGATAGGAAAAGGTGAAAAA
TATGCTGAGATGAAATCAATAGAATTACAAAGGCCTCTTAATCAACTAGAGAATTTGTCTAAACCAACTATAGCTGCTAT
TAATGGGTTAGCACTAGGCGGAGGTTGTGAATTAGCACTTGCTTGTGATTTAAGAGTAATAGAAGAACAAGCCTTAATTG
GATTACCAGAAATTACGTTAGGTCTATTTCCTGGGGCTGGAGGAACTCAGCGCTTACCTAGATTAATTGGAGAAGGAAAA
GCAAAAGAGATGATGTTCACAGGAAAACCAATTACAGCGAAAGAAGCAAAAGAAATAAATTTAGTTAACTACATTACCTC
TCGAGGAGAGGCGTTAAATAAAGCAAAAGAGATTGCTAAAGATATAAGTGAATTTTCTCTTCCAGCACTTTCCTATATGA
AGTTAGCAATTCGTGAAGGTTTGGCAGTACCTTTACAAGAAGGGCTTCAGATCGAAGCGCGTTATTTTGGGAAAGTGTTT
CAAACAGAAGATGTGAAGGAGGGGGTAAAGGCATTTATTGAAAAAAGAGTACCGCGTTTCACCAATAAATAA

Upstream 100 bases:

>100_bases
TTTCTAAAAAGGGAAATATATACACTAATTCAGGATAATAGTGAATAAATCGAAGAAACTTGAACTTGAGAAAAATTAGG
GATTTAAAAGGGGACTTTTT

Downstream 100 bases:

>100_bases
AAAAGTATTGGGGTGTATTTTACAATTGCCCACACAGATGCTACTTTTAAAGCTATTTATTTGATTCTTAAAATATCTTT
CAGAGCTGTTTTATCTAAGA

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKNERLVICSKKGSSAVITIQNPPVNALSLEVVQQLINVLEEIEMDDDIAVVIITGIGGKAFVAGGDIKEFPGWIGKGEK
YAEMKSIELQRPLNQLENLSKPTIAAINGLALGGGCELALACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGK
AKEMMFTGKPITAKEAKEINLVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIREGLAVPLQEGLQIEARYFGKVF
QTEDVKEGVKAFIEKRVPRFTNK

Sequences:

>Translated_263_residues
MKNERLVICSKKGSSAVITIQNPPVNALSLEVVQQLINVLEEIEMDDDIAVVIITGIGGKAFVAGGDIKEFPGWIGKGEK
YAEMKSIELQRPLNQLENLSKPTIAAINGLALGGGCELALACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGK
AKEMMFTGKPITAKEAKEINLVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIREGLAVPLQEGLQIEARYFGKVF
QTEDVKEGVKAFIEKRVPRFTNK
>Mature_263_residues
MKNERLVICSKKGSSAVITIQNPPVNALSLEVVQQLINVLEEIEMDDDIAVVIITGIGGKAFVAGGDIKEFPGWIGKGEK
YAEMKSIELQRPLNQLENLSKPTIAAINGLALGGGCELALACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGK
AKEMMFTGKPITAKEAKEINLVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIREGLAVPLQEGLQIEARYFGKVF
QTEDVKEGVKAFIEKRVPRFTNK

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=265, Percent_Identity=35.8490566037736, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI4502327, Length=246, Percent_Identity=39.0243902439024, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI68989263, Length=189, Percent_Identity=35.978835978836, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=39.3258426966292, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=29.2, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI150378541, Length=243, Percent_Identity=31.6872427983539, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI37594469, Length=153, Percent_Identity=38.562091503268, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI37594471, Length=153, Percent_Identity=38.562091503268, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI70995211, Length=241, Percent_Identity=29.8755186721992, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI4757968, Length=245, Percent_Identity=22.8571428571429, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI49355787, Length=245, Percent_Identity=22.8571428571429, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI25453481, Length=245, Percent_Identity=22.8571428571429, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI51538573, Length=245, Percent_Identity=22.8571428571429, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI4757966, Length=227, Percent_Identity=23.3480176211454, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI51538581, Length=227, Percent_Identity=23.3480176211454, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI261878539, Length=95, Percent_Identity=41.0526315789474, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221136756, Length=236, Percent_Identity=24.1525423728814, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI221136753, Length=236, Percent_Identity=24.1525423728814, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI62530384, Length=256, Percent_Identity=24.609375, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221307494, Length=236, Percent_Identity=24.1525423728814, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI213417737, Length=228, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI116235487, Length=188, Percent_Identity=27.1276595744681, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI260274832, Length=205, Percent_Identity=27.3170731707317, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI45643119, Length=205, Percent_Identity=27.3170731707317, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI260275230, Length=205, Percent_Identity=27.3170731707317, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI157694520, Length=155, Percent_Identity=27.741935483871, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI295842266, Length=128, Percent_Identity=32.03125, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=39.6153846153846, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788682, Length=221, Percent_Identity=38.9140271493213, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=31.8725099601594, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=35.7954545454545, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87082183, Length=243, Percent_Identity=27.5720164609054, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787660, Length=249, Percent_Identity=30.1204819277108, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=37.7952755905512, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI25145438, Length=261, Percent_Identity=37.1647509578544, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=34.5454545454545, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=36.8131868131868, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144160, Length=209, Percent_Identity=31.1004784688995, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25144157, Length=209, Percent_Identity=31.1004784688995, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=165, Percent_Identity=36.969696969697, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=165, Percent_Identity=36.969696969697, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=165, Percent_Identity=36.969696969697, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=232, Percent_Identity=31.8965517241379, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=255, Percent_Identity=28.2352941176471, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=255, Percent_Identity=25.0980392156863, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=27.0916334661355, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=195, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=202, Percent_Identity=27.7227722772277, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25141363, Length=186, Percent_Identity=27.4193548387097, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=198, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=39.9193548387097, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=39.9193548387097, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=38.8, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI21357171, Length=264, Percent_Identity=31.8181818181818, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24650670, Length=232, Percent_Identity=31.4655172413793, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922422, Length=247, Percent_Identity=27.1255060728745, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=38.5057471264368, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=38.5057471264368, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=38.5057471264368, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24654903, Length=251, Percent_Identity=23.1075697211155, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28571730, Length=154, Percent_Identity=33.1168831168831, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28571729, Length=154, Percent_Identity=33.1168831168831, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19920382, Length=244, Percent_Identity=26.6393442622951, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921018, Length=208, Percent_Identity=32.2115384615385, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583165, Length=240, Percent_Identity=27.9166666666667, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28550; Mature: 28550

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNERLVICSKKGSSAVITIQNPPVNALSLEVVQQLINVLEEIEMDDDIAVVIITGIGGK
CCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
AFVAGGDIKEFPGWIGKGEKYAEMKSIELQRPLNQLENLSKPTIAAINGLALGGGCELAL
EEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEECCCCCEEE
ACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGKAKEMMFTGKPITAKEAKEIN
EECHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
LVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIREGLAVPLQEGLQIEARYFGKVF
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHCCCEEEHHHHHHHH
QTEDVKEGVKAFIEKRVPRFTNK
CCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNERLVICSKKGSSAVITIQNPPVNALSLEVVQQLINVLEEIEMDDDIAVVIITGIGGK
CCCCEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
AFVAGGDIKEFPGWIGKGEKYAEMKSIELQRPLNQLENLSKPTIAAINGLALGGGCELAL
EEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEECCCCCEEE
ACDLRVIEEQALIGLPEITLGLFPGAGGTQRLPRLIGEGKAKEMMFTGKPITAKEAKEIN
EECHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
LVNYITSRGEALNKAKEIAKDISEFSLPALSYMKLAIREGLAVPLQEGLQIEARYFGKVF
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHCCCEEEHHHHHHHH
QTEDVKEGVKAFIEKRVPRFTNK
CCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]