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The map label for this gene is gmuR [H]

Identifier: 30263364

GI number: 30263364

Start: 3180623

End: 3181360

Strand: Reverse

Name: gmuR [H]

Synonym: BA_3458

Alternate gene names: 30263364

Gene position: 3181360-3180623 (Counterclockwise)

Preceding gene: 30263365

Following gene: 30263362

Centisome position: 60.86

GC content: 39.84

Gene sequence:

>738_bases
ATGGCAAGCGGATCCACACAAGTAAAATACCTCGGCATTTATCAAAAAATGAAACAGCAAATTTTAGACGGCGAATATAA
GATTAACGAAAAAATTCCAAGTAGCCCCGTCCTTGCTGAAGAATTTGATGTTTCTGTCCTTACTATAAAAAAAGCGCTGG
ATCTGTTAGTTAGAGACGGCTACATCATTCGCCGGCGCGGAAGTGGAACAGTCGTTCAAGATTGGCGTCAGCAGGAAAAA
GCACGAATGATTCAAACTTTAACAGGTACAAAAGCTGTTTATGGCAGCGAGGTAGAAAGTAAAATTATTGAGTTTACGAT
TGTCGGTGCCGATGAAATTATTGCTGAAAAATTGGGCATTTCAGTAGGAGATTTTGTATATAAAATCATCCGCCTCCGCA
TCATTCACAGTATTCCAACGATTATGGAGCATACATGGATGCCGATTTCGGTCATTCCAGGTGTTGAAGTTTCTGTTTTA
GAGGAATCAATCTACTCCCACATTCAAAATAAACTAGGCCTTCAAGTGGGAACATCCGTTGTTAGGGTAAAAGGAATTCG
CCCGGATGATAAAGAAAAGCAGTTTATGAACTTAACAAATCAAGATTTCCTAATGAGAGTGGAACAGGTGGCCTACTTAA
CGGATGGACGCACTTTTGAATACTCTTACGCCGATCACTTGCCGGAAACCTTTGAATTCGAAACAGTTATTACTGCAAAA
AGTTATAAAGAGGCATAA

Upstream 100 bases:

>100_bases
TAGAAAACGGATTTGCTGCGCCATCACCTTCTGTTTACAAACTTGAGGCTTTAAACTTAAATGAATAGAAGAGGGAATAC
AGCATAAAGGAGGTATGGAA

Downstream 100 bases:

>100_bases
AAAATAGGTTGAGGCGCGTATTGCCTCAACCTATTTTTTGTCTTCAATATAGTAGAAACAGCTATTATTCATCGGCTGTA
ATAAGCATGGATGTGTGCCC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: Glucomannan utilization operon repressor; Glucomannan utilization protein R [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MASGSTQVKYLGIYQKMKQQILDGEYKINEKIPSSPVLAEEFDVSVLTIKKALDLLVRDGYIIRRRGSGTVVQDWRQQEK
ARMIQTLTGTKAVYGSEVESKIIEFTIVGADEIIAEKLGISVGDFVYKIIRLRIIHSIPTIMEHTWMPISVIPGVEVSVL
EESIYSHIQNKLGLQVGTSVVRVKGIRPDDKEKQFMNLTNQDFLMRVEQVAYLTDGRTFEYSYADHLPETFEFETVITAK
SYKEA

Sequences:

>Translated_245_residues
MASGSTQVKYLGIYQKMKQQILDGEYKINEKIPSSPVLAEEFDVSVLTIKKALDLLVRDGYIIRRRGSGTVVQDWRQQEK
ARMIQTLTGTKAVYGSEVESKIIEFTIVGADEIIAEKLGISVGDFVYKIIRLRIIHSIPTIMEHTWMPISVIPGVEVSVL
EESIYSHIQNKLGLQVGTSVVRVKGIRPDDKEKQFMNLTNQDFLMRVEQVAYLTDGRTFEYSYADHLPETFEFETVITAK
SYKEA
>Mature_244_residues
ASGSTQVKYLGIYQKMKQQILDGEYKINEKIPSSPVLAEEFDVSVLTIKKALDLLVRDGYIIRRRGSGTVVQDWRQQEKA
RMIQTLTGTKAVYGSEVESKIIEFTIVGADEIIAEKLGISVGDFVYKIIRLRIIHSIPTIMEHTWMPISVIPGVEVSVLE
ESIYSHIQNKLGLQVGTSVVRVKGIRPDDKEKQFMNLTNQDFLMRVEQVAYLTDGRTFEYSYADHLPETFEFETVITAKS
YKEA

Specific function: Transcriptional repressor of the gmuBACDREFG operon which is involved in the uptake and degradation of glucomannan [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786950, Length=234, Percent_Identity=26.0683760683761, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1790118, Length=218, Percent_Identity=29.3577981651376, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082252, Length=209, Percent_Identity=25.8373205741627, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790540, Length=225, Percent_Identity=24.8888888888889, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 27845; Mature: 27713

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGSTQVKYLGIYQKMKQQILDGEYKINEKIPSSPVLAEEFDVSVLTIKKALDLLVRDG
CCCCCCEEHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCC
YIIRRRGSGTVVQDWRQQEKARMIQTLTGTKAVYGSEVESKIIEFTIVGADEIIAEKLGI
EEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHEEEEEEEECHHHHHHHHHCC
SVGDFVYKIIRLRIIHSIPTIMEHTWMPISVIPGVEVSVLEESIYSHIQNKLGLQVGTSV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHCHHHCCEE
VRVKGIRPDDKEKQFMNLTNQDFLMRVEQVAYLTDGRTFEYSYADHLPETFEFETVITAK
EEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCEEEEEEEECC
SYKEA
CCCCC
>Mature Secondary Structure 
ASGSTQVKYLGIYQKMKQQILDGEYKINEKIPSSPVLAEEFDVSVLTIKKALDLLVRDG
CCCCCEEHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCC
YIIRRRGSGTVVQDWRQQEKARMIQTLTGTKAVYGSEVESKIIEFTIVGADEIIAEKLGI
EEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHEEEEEEEECHHHHHHHHHCC
SVGDFVYKIIRLRIIHSIPTIMEHTWMPISVIPGVEVSVLEESIYSHIQNKLGLQVGTSV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHCHHHCCEE
VRVKGIRPDDKEKQFMNLTNQDFLMRVEQVAYLTDGRTFEYSYADHLPETFEFETVITAK
EEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCEEEEEEEECC
SYKEA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]