BacMap

Definition	Bacillus anthracis str. Ames, complete genome.
Accession	NC_003997
Length	5,227,293

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The map label for this gene is ytbE [H]

Identifier: 30263352

GI number: 30263352

Start: 3170445

End: 3171284

Strand: Reverse

Name: ytbE [H]

Synonym: BA_3446

Alternate gene names: 30263352

Gene position: 3171284-3170445 (Counterclockwise)

Preceding gene: 30263353

Following gene: 30263351

Centisome position: 60.67

GC content: 35.6

Gene sequence:

>840_bases
ATGAAAAACTTACAAAGTACAACAAAATTGCATAACGGTGTAGAAATGCCTTGGTTCGGTTTAGGCGTATTTAAAGTAGA
AGAAGGACCAGAACTTGTAGAAGCAATTAAATCAGCAATTAAGACAGGATACCGTAGCATCGATACTGCTGCGATTTATG
GAAATGAAGCAGCTGTAGGTGAAGGAATTCGCGCTGGTATTGAAGCGACTGGAATTTCAAGAGAAGAATTATTTATCACT
TCAAAAGTATGGAACGCAGATCAAGGATACAAAGAAACAATTGCTGCATATGAAGAGAGTTTAAAAAAATTACAACTAGA
TTATTTAGATTTATATCTTGTTCACTGGCCTGTAGAAGGGAAATATAAAGATACGTGGAGAGCGTTAGAAACGCTTTATA
AAGAAAAGCGTGTACGTGCAATTGGCGTAAGTAACTTCCAAATTCATCACCTGCAAGATGTAATACAAGATGCAGAAATT
AAGCCAATGATCAATCAAGTAGAATACCATCCTCGTTTAACGCAAAAAGAGTTACAAGCTTTCTGTAAAGAACAAGGTAT
TCAAATGGAAGCATGGTCACCACTTATGCAAGGTCAATTATTAGATAATGAAACATTACAAGCAATTGCTGAGAAACACG
GTAAAACAACAGCGCAAGTTATTTTACGTTGGGATCTTCAAAATGGAGTAATTACAATTCCGAAATCAACGAAAGAACAC
CGCATTATTGCAAATGCTGATGTATTTAACTTTGAATTAACGAAAGAAGATATGGAGAAAATCGATGCGTTAAATGAAAA
TCACCGCGTTGGTCCAGATCCTGATAACTTTGATTTCTAA

Upstream 100 bases:

>100_bases
TGGGTAGGCGGCATTATGGTAGTAGGTGCTGTTATTTTAACAGCATGGAGTATGTCATTAGAAAAACGAGATCAAGTAAA
ATAAAAGGAGAGAAAATATA

Downstream 100 bases:

>100_bases
GATTAAAAAATGAGTAAAAAGAGTGCAAAGTTGCACTCTTTTTTATTTTCTATTCAATAAAATGTTGCTGTTTATATTAT
GACTTTTCAATTACTTTAGG

Product: aldo/keto reductase family oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKNLQSTTKLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKTGYRSIDTAAIYGNEAAVGEGIRAGIEATGISREELFIT
SKVWNADQGYKETIAAYEESLKKLQLDYLDLYLVHWPVEGKYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIQDAEI
KPMINQVEYHPRLTQKELQAFCKEQGIQMEAWSPLMQGQLLDNETLQAIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
RIIANADVFNFELTKEDMEKIDALNENHRVGPDPDNFDF

Sequences:

>Translated_279_residues
MKNLQSTTKLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKTGYRSIDTAAIYGNEAAVGEGIRAGIEATGISREELFIT
SKVWNADQGYKETIAAYEESLKKLQLDYLDLYLVHWPVEGKYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIQDAEI
KPMINQVEYHPRLTQKELQAFCKEQGIQMEAWSPLMQGQLLDNETLQAIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
RIIANADVFNFELTKEDMEKIDALNENHRVGPDPDNFDF
>Mature_279_residues
MKNLQSTTKLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKTGYRSIDTAAIYGNEAAVGEGIRAGIEATGISREELFIT
SKVWNADQGYKETIAAYEESLKKLQLDYLDLYLVHWPVEGKYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIQDAEI
KPMINQVEYHPRLTQKELQAFCKEQGIQMEAWSPLMQGQLLDNETLQAIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
RIIANADVFNFELTKEDMEKIDALNENHRVGPDPDNFDF

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=295, Percent_Identity=40, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI5174391, Length=295, Percent_Identity=40, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI223468663, Length=298, Percent_Identity=38.5906040268456, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI310109922, Length=300, Percent_Identity=39, Blast_Score=198, Evalue=6e-51,
Organism=Homo sapiens, GI310109920, Length=320, Percent_Identity=38.125, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI45446745, Length=320, Percent_Identity=37.8125, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI4503285, Length=320, Percent_Identity=37.8125, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI5453543, Length=319, Percent_Identity=37.6175548589342, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI93277124, Length=305, Percent_Identity=36.7213114754098, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=38.7205387205387, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI24497583, Length=302, Percent_Identity=36.7549668874172, Blast_Score=186, Evalue=1e-47,
Organism=Homo sapiens, GI291291012, Length=278, Percent_Identity=38.4892086330935, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI4502049, Length=299, Percent_Identity=38.7959866220736, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=35.472972972973, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=34.1818181818182, Blast_Score=154, Evalue=6e-38,
Organism=Homo sapiens, GI300116271, Length=274, Percent_Identity=33.9416058394161, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI310109924, Length=160, Percent_Identity=39.375, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI310109926, Length=123, Percent_Identity=43.0894308943089, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI207028673, Length=123, Percent_Identity=42.2764227642276, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI87082198, Length=276, Percent_Identity=45.6521739130435, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1786400, Length=263, Percent_Identity=37.6425855513308, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1788081, Length=274, Percent_Identity=33.2116788321168, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1788070, Length=219, Percent_Identity=27.3972602739726, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081735, Length=282, Percent_Identity=22.3404255319149, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71998625, Length=273, Percent_Identity=41.7582417582418, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17537077, Length=295, Percent_Identity=40.3389830508475, Blast_Score=201, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17564128, Length=299, Percent_Identity=35.7859531772575, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17537075, Length=304, Percent_Identity=39.4736842105263, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17550248, Length=300, Percent_Identity=37.3333333333333, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17561298, Length=273, Percent_Identity=35.8974358974359, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17538386, Length=275, Percent_Identity=37.0909090909091, Blast_Score=177, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17552492, Length=273, Percent_Identity=32.967032967033, Blast_Score=177, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17537079, Length=312, Percent_Identity=33.0128205128205, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17562292, Length=275, Percent_Identity=32.3636363636364, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17561300, Length=275, Percent_Identity=33.8181818181818, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17566692, Length=286, Percent_Identity=32.5174825174825, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321896, Length=317, Percent_Identity=36.9085173501577, Blast_Score=198, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6320576, Length=300, Percent_Identity=36.3333333333333, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6322556, Length=261, Percent_Identity=36.3984674329502, Blast_Score=184, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324694, Length=298, Percent_Identity=36.241610738255, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6319625, Length=296, Percent_Identity=36.4864864864865, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6320079, Length=286, Percent_Identity=33.9160839160839, Blast_Score=138, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24663317, Length=298, Percent_Identity=39.9328859060403, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24662789, Length=294, Percent_Identity=39.1156462585034, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24644950, Length=299, Percent_Identity=37.123745819398, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24657054, Length=292, Percent_Identity=38.013698630137, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI20129731, Length=296, Percent_Identity=36.8243243243243, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI221378297, Length=319, Percent_Identity=34.7962382445141, Blast_Score=187, Evalue=5e-48,
Organism=Drosophila melanogaster, GI45553081, Length=295, Percent_Identity=33.8983050847458, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24662781, Length=295, Percent_Identity=40.6779661016949, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21356425, Length=294, Percent_Identity=35.0340136054422, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24662785, Length=291, Percent_Identity=40.893470790378, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI281366140, Length=256, Percent_Identity=33.59375, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 31831; Mature: 31831

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLQSTTKLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKTGYRSIDTAAIYGNEAAVG
CCCCHHHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHC
EGIRAGIEATGISREELFITSKVWNADQGYKETIAAYEESLKKLQLDYLDLYLVHWPVEG
CHHHCCCHHCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCC
KYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIQDAEIKPMINQVEYHPRLTQKELQA
CHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHH
FCKEQGIQMEAWSPLMQGQLLDNETLQAIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
HHHHCCCCCHHCCHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCEEECCCCCCCC
RIIANADVFNFELTKEDMEKIDALNENHRVGPDPDNFDF
EEEECCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNLQSTTKLHNGVEMPWFGLGVFKVEEGPELVEAIKSAIKTGYRSIDTAAIYGNEAAVG
CCCCHHHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHC
EGIRAGIEATGISREELFITSKVWNADQGYKETIAAYEESLKKLQLDYLDLYLVHWPVEG
CHHHCCCHHCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCC
KYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVIQDAEIKPMINQVEYHPRLTQKELQA
CHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHH
FCKEQGIQMEAWSPLMQGQLLDNETLQAIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
HHHHCCCCCHHCCHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCEEECCCCCCCC
RIIANADVFNFELTKEDMEKIDALNENHRVGPDPDNFDF
EEEECCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]