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The map label for this gene is proB

Identifier: 30262949

GI number: 30262949

Start: 2759899

End: 2761002

Strand: Reverse

Name: proB

Synonym: BA_2993

Alternate gene names: 30262949

Gene position: 2761002-2759899 (Counterclockwise)

Preceding gene: 30262951

Following gene: 30262948

Centisome position: 52.82

GC content: 41.39

Gene sequence:

>1104_bases
GTGAAAAAACAACGAATTGTTGTAAAGATTGGGAGCAGTTCTTTGGCGGATAGTCACGAAGGCATTTCTAAAGAACAACT
TTCAGATCATGTTGCGGCATTAGCTCGATTGAAAGAGGAAGGGCATGAAGTGTTGTTAATTACATCTGGGGCCGTCGCAG
CAGGTTTTTCAGCTCTAGGGTACCCTAGTAGACCTGTAACAATTAAAGGAAAACAGGCAGCTGCGGCTGTCGGACAAAGT
TTGTTAATGCAGGCGTACACGGAGGAATTTCGTAAATACGGTATCGTTACTGCGCAACTATTGCTGACTAGAAGTGATTT
TTCTAGAAAAGAACAATATAGTAACGCTTACGCGACTTTAGGAGAGTTACTAAACCGTTCTGCTCTTCCAATCATTAATG
AAAATGATTCCATTTCTTTAGAGGAGCTAACGTTCGGTGATAATGATATGCTGTCAGCTTTAGTAAGCGGACTTGTTTCG
GCGGATATGCTGATGATTTTTACAGATGTGAACGGTTTATATGATAAAAATCCTCAGAAAAATGAGGATGCGAAAAAATA
TTATTTCCTTCCTGAAGTTACAGAAGAAATCGCTTCTCTTGCAGGAGATGCTGGCTCCAAACTTGGGACTGGCGGAATGA
AATCAAAAGTCGATGCTGCAAAGACAGCACTCTCTCTTGGTGTCAGTGTATTTATCGGAACAGGACGTGGACAAGAGAAA
TTTGTAGATGTTTTGAAGGGGAAAGGTGATGGAACGTATGTCGGAAATGCCCCTCAAAAAGAAATGAAGATAAATAAGCA
ATGGATCGCTTTGCATTCGGTTGTTAGCGGACAAATTGAAATAGACGCCGGAGCAGCTACAGCTATCATTCAGCATGGCA
AGAGTCTTCTTCCTGCTGGTGTTACAAATGTTTCAGGGTTTTTCCAAGTGGGCGAAGTCGTAGAAGTAATGACGCAACAA
GGACGCGTGATTGGAAAAGGACAATGCACATATAGTGCAGAGGAGCTAAGGGATGTAAAAGGTATGCAAAGCCAACAGAT
TCAAGCAAGAGGCGAAAGACATAATTATGAAGTCATTCATAGAGATTATTGGGTTTCATTTTAA

Upstream 100 bases:

>100_bases
ACTAGGTGCAGCACTGATAAAAATAGAAATCGCTGCAAGCTGTATGAATAAAAGTGTAATGTATAAAAAAACTTTGATTT
TAGACTTGGGGGATATAAAA

Downstream 100 bases:

>100_bases
GAAGATGGATCAAAAAGGAGGAAATAAAAATGAATGAAGTGTTAGCAAAGGGAAAGAAGGCGAAAGAGATCGCTAGAGAG
CTAGTGCTTAAATCTACAGA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKKQRIVVKIGSSSLADSHEGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQS
LLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVS
ADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEVTEEIASLAGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEK
FVDVLKGKGDGTYVGNAPQKEMKINKQWIALHSVVSGQIEIDAGAATAIIQHGKSLLPAGVTNVSGFFQVGEVVEVMTQQ
GRVIGKGQCTYSAEELRDVKGMQSQQIQARGERHNYEVIHRDYWVSF

Sequences:

>Translated_367_residues
MKKQRIVVKIGSSSLADSHEGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQS
LLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVS
ADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEVTEEIASLAGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEK
FVDVLKGKGDGTYVGNAPQKEMKINKQWIALHSVVSGQIEIDAGAATAIIQHGKSLLPAGVTNVSGFFQVGEVVEVMTQQ
GRVIGKGQCTYSAEELRDVKGMQSQQIQARGERHNYEVIHRDYWVSF
>Mature_367_residues
MKKQRIVVKIGSSSLADSHEGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQS
LLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVS
ADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEVTEEIASLAGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEK
FVDVLKGKGDGTYVGNAPQKEMKINKQWIALHSVVSGQIEIDAGAATAIIQHGKSLLPAGVTNVSGFFQVGEVVEVMTQQ
GRVIGKGQCTYSAEELRDVKGMQSQQIQARGERHNYEVIHRDYWVSF

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=286, Percent_Identity=32.1678321678322, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI62912457, Length=284, Percent_Identity=32.3943661971831, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=38.7362637362637, Blast_Score=253, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17569967, Length=271, Percent_Identity=35.0553505535055, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17569969, Length=271, Percent_Identity=35.0553505535055, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320506, Length=418, Percent_Identity=34.9282296650718, Blast_Score=210, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6321822, Length=401, Percent_Identity=33.4164588528678, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=35.8885017421603, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_BACAN (Q81P26)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_845326.1
- RefSeq:   YP_019636.2
- ProteinModelPortal:   Q81P26
- SMR:   Q81P26
- IntAct:   Q81P26
- EnsemblBacteria:   EBBACT00000012665
- EnsemblBacteria:   EBBACT00000014997
- EnsemblBacteria:   EBBACT00000024286
- GeneID:   1086339
- GeneID:   2819367
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_2993
- KEGG:   bar:GBAA_2993
- KEGG:   bat:BAS2782
- TIGR:   BA_2993
- TIGR:   GBAA_2993
- GeneTree:   EBGT00050000001887
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   BANT260799:BAS2782-MONOMER
- BioCyc:   BANT261594:GBAA2993-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39600; Mature: 39600

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQRIVVKIGSSSLADSHEGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALG
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHCC
YPSRPVTIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATL
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
GELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQK
HHHHCCCCCCEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
NEDAKKYYFLPEVTEEIASLAGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEK
CCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCHHH
FVDVLKGKGDGTYVGNAPQKEMKINKQWIALHSVVSGQIEIDAGAATAIIQHGKSLLPAG
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHCCCCCCCCC
VTNVSGFFQVGEVVEVMTQQGRVIGKGQCTYSAEELRDVKGMQSQQIQARGERHNYEVIH
CCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEEE
RDYWVSF
EEEEECC
>Mature Secondary Structure
MKKQRIVVKIGSSSLADSHEGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALG
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHCC
YPSRPVTIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATL
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
GELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQK
HHHHCCCCCCEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
NEDAKKYYFLPEVTEEIASLAGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEK
CCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCHHH
FVDVLKGKGDGTYVGNAPQKEMKINKQWIALHSVVSGQIEIDAGAATAIIQHGKSLLPAG
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHCCCCCCCCC
VTNVSGFFQVGEVVEVMTQQGRVIGKGQCTYSAEELRDVKGMQSQQIQARGERHNYEVIH
CCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEEE
RDYWVSF
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629