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The map label for this gene is trmE

Identifier: 77748780

GI number: 77748780

Start: 5168701

End: 5170041

Strand: Reverse

Name: trmE

Synonym: XAC4370

Alternate gene names: 77748780

Gene position: 5170041-5168701 (Counterclockwise)

Preceding gene: 77748781

Following gene: 77748779

Centisome position: 99.89

GC content: 65.4

Gene sequence:

>1341_bases
ATGTCGTCTTCCACCTCGACCATCGTTGCCATCGCCAGTGCTGCCGGAATCGGCGGCGTGGGCATCGTGCGCTTGTCCGG
GCCGCAGTCGGTGCAGATCGCAGCGCACCTCGGAATTGCGCGGATGCAGCCGCGGCATGCGCACTACGCGCGTTTTCGCG
ATGGGCACGGCGCCGTCATCGACGATGGCATTGCGCTGTGGTTCAACGCGCCGCACAGCTTCACCGGCGAAGATGTCGTC
GAGCTGCAAGGTCATGGCAGCCCGGTGCTGTTGCGGCAACTGGTTGCCCGCTGCATCGAACTGGGTGCACGTCAGGCACG
CGCCGGCGAATTCAGCGAACGTGCGTTTCTCAACGGCAAGCTCGATCTTGCGCAGGCCGAGGCCATTGCCGACTTGATCG
CGGCCGGCGACCTGCGGGCGGCACGCGCCGCGCGGCGCGCACTGGACGGCGTGTTTTCGCAACGGGTCGATGCACTGGCC
GAGACCCTGACGCGCCTGCGTATCCATGTCGAGGCAGCCATCGACTTTGCCGATGAGCCGCTCGACACCTTGGGCGGCAA
TCAAGTACGCGATGGTCTGGGTCAGGCGCGCAACGTGCTCGCACAGCTGCTGCGCGATGCCGAACGCGGGCGTAAGTTGC
GCGACGGCCTGCATGCGGTGTTGATCGGTCCGCCCAATGCCGGCAAGAGTTCCCTGCTCAATGCGCTGGCCGGAAGCGAC
CGCGCAATCGTCACCGATGTTGCCGGCACCACCCGCGACACCTTGCACGAAGCCATCCAGCTGGACGGTTTCGAGTTGAC
CTTGGTCGATACCGCCGGCCTGCGCGATGGCGGCGATGCGATCGAACGCGAAGGCATGCGCCGCGCACGCGCCGAACTGG
AGCGCGCCGACCTGGCGCTGGTGGTGCTGGATGCACGCGACCTGCAGGCCGCCCGCGATGCCATCGGCGATGCCATCGAC
ACGGTGCCGCGTCAGCTATGGATCCACAACAAATGCGATCTGCTCGGCAATGCCGCATCGCTCGATGCCAATGCGATCGC
TGTCTCTGCCGTGACCGGACAAGGACTGGAGCAATTGCACATCCGCCTGCGCGCGCTTGCGCTCGGCGATGGCGTGGACA
GCGTCGACGGCGAGTTCTCTGCTCGCACGCGGCATGTTCAGGCGCTGCGTCGCGCCGAACAGCACGCCGATGCTGCCGAT
CTGGAGCTCGGTTTCGAACAACTGGAACTGGCTGCCGAAGAACTGCGTCTGGCACATGAAGCGCTCGGCGAGATCACCGG
AAAGCTCAGCGCCGACGACCTGCTCGGCAAGATCTTCTCGAGTTTTTGCATCGGCAAGTGA

Upstream 100 bases:

>100_bases
TAGGCGACTCAAAAGTGCCCGCAAACATGTGGCTCGGCACTGCTGGCCGCGCCGTTTAAAATCCACGTGTCCTTGTTCAA
TCCACCATAGAGCAGTGTCC

Downstream 100 bases:

>100_bases
TGCGGTGGTGCCCAGCGCGATGAGGCGCGCGGAAACCGTCGGAATCAGCCGCACCCAAACGCATCTGCCGCCGCACATCA
GCTGCGTGGACGGCGATGCG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSSSTSTIVAIASAAGIGGVGIVRLSGPQSVQIAAHLGIARMQPRHAHYARFRDGHGAVIDDGIALWFNAPHSFTGEDVV
ELQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGKLDLAQAEAIADLIAAGDLRAARAARRALDGVFSQRVDALA
ETLTRLRIHVEAAIDFADEPLDTLGGNQVRDGLGQARNVLAQLLRDAERGRKLRDGLHAVLIGPPNAGKSSLLNALAGSD
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLALVVLDARDLQAARDAIGDAID
TVPRQLWIHNKCDLLGNAASLDANAIAVSAVTGQGLEQLHIRLRALALGDGVDSVDGEFSARTRHVQALRRAEQHADAAD
LELGFEQLELAAEELRLAHEALGEITGKLSADDLLGKIFSSFCIGK

Sequences:

>Translated_446_residues
MSSSTSTIVAIASAAGIGGVGIVRLSGPQSVQIAAHLGIARMQPRHAHYARFRDGHGAVIDDGIALWFNAPHSFTGEDVV
ELQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGKLDLAQAEAIADLIAAGDLRAARAARRALDGVFSQRVDALA
ETLTRLRIHVEAAIDFADEPLDTLGGNQVRDGLGQARNVLAQLLRDAERGRKLRDGLHAVLIGPPNAGKSSLLNALAGSD
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLALVVLDARDLQAARDAIGDAID
TVPRQLWIHNKCDLLGNAASLDANAIAVSAVTGQGLEQLHIRLRALALGDGVDSVDGEFSARTRHVQALRRAEQHADAAD
LELGFEQLELAAEELRLAHEALGEITGKLSADDLLGKIFSSFCIGK
>Mature_445_residues
SSSTSTIVAIASAAGIGGVGIVRLSGPQSVQIAAHLGIARMQPRHAHYARFRDGHGAVIDDGIALWFNAPHSFTGEDVVE
LQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGKLDLAQAEAIADLIAAGDLRAARAARRALDGVFSQRVDALAE
TLTRLRIHVEAAIDFADEPLDTLGGNQVRDGLGQARNVLAQLLRDAERGRKLRDGLHAVLIGPPNAGKSSLLNALAGSDR
AIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLALVVLDARDLQAARDAIGDAIDT
VPRQLWIHNKCDLLGNAASLDANAIAVSAVTGQGLEQLHIRLRALALGDGVDSVDGEFSARTRHVQALRRAEQHADAADL
ELGFEQLELAAEELRLAHEALGEITGKLSADDLLGKIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=466, Percent_Identity=39.6995708154506, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI193082988, Length=498, Percent_Identity=37.5502008032129, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI193082993, Length=455, Percent_Identity=38.6813186813187, Blast_Score=234, Evalue=9e-62,
Organism=Escherichia coli, GI2367268, Length=456, Percent_Identity=49.780701754386, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI87082120, Length=90, Percent_Identity=40, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=459, Percent_Identity=31.8082788671024, Blast_Score=187, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=498, Percent_Identity=28.1124497991968, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI45550813, Length=481, Percent_Identity=33.4719334719335, Blast_Score=208, Evalue=6e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_XANAC (Q8PEH9)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644664.2
- ProteinModelPortal:   Q8PEH9
- SMR:   Q8PEH9
- GeneID:   1158441
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC4370
- NMPDR:   fig|190486.1.peg.4308
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- ProtClustDB:   PRK05291
- BioCyc:   XAXO190486:XAC4370-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 47326; Mature: 47195

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: BINDING 26-26 BINDING 83-83 BINDING 122-122 BINDING 448-448

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSTSTIVAIASAAGIGGVGIVRLSGPQSVQIAAHLGIARMQPRHAHYARFRDGHGAVI
CCCCCCEEEEEEHHCCCCCEEEEEECCCCCEEHHHHHCHHHCCCCHHHHHHEECCCCCEE
DDGIALWFNAPHSFTGEDVVELQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGK
ECCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHCCCC
LDLAQAEAIADLIAAGDLRAARAARRALDGVFSQRVDALAETLTRLRIHVEAAIDFADEP
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LDTLGGNQVRDGLGQARNVLAQLLRDAERGRKLRDGLHAVLIGPPNAGKSSLLNALAGSD
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCEEEEECCCCCCHHHHHHHHCCCC
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLAL
CEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
VVLDARDLQAARDAIGDAIDTVPRQLWIHNKCDLLGNAASLDANAIAVSAVTGQGLEQLH
EEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEEEECCCCCHHHHH
IRLRALALGDGVDSVDGEFSARTRHVQALRRAEQHADAADLELGFEQLELAAEELRLAHE
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHH
ALGEITGKLSADDLLGKIFSSFCIGK
HHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSSTSTIVAIASAAGIGGVGIVRLSGPQSVQIAAHLGIARMQPRHAHYARFRDGHGAVI
CCCCCEEEEEEHHCCCCCEEEEEECCCCCEEHHHHHCHHHCCCCHHHHHHEECCCCCEE
DDGIALWFNAPHSFTGEDVVELQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGK
ECCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHCCCC
LDLAQAEAIADLIAAGDLRAARAARRALDGVFSQRVDALAETLTRLRIHVEAAIDFADEP
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LDTLGGNQVRDGLGQARNVLAQLLRDAERGRKLRDGLHAVLIGPPNAGKSSLLNALAGSD
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCEEEEECCCCCCHHHHHHHHCCCC
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLAL
CEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
VVLDARDLQAARDAIGDAIDTVPRQLWIHNKCDLLGNAASLDANAIAVSAVTGQGLEQLH
EEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEEEECCCCCHHHHH
IRLRALALGDGVDSVDGEFSARTRHVQALRRAEQHADAADLELGFEQLELAAEELRLAHE
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHH
ALGEITGKLSADDLLGKIFSSFCIGK
HHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217