Click here to switch to the map view.

The map label for this gene is metL [H]

Identifier: 77748690

GI number: 77748690

Start: 3554500

End: 3555543

Strand: Reverse

Name: metL [H]

Synonym: XAC3038

Alternate gene names: 77748690

Gene position: 3555543-3554500 (Counterclockwise)

Preceding gene: 21243766

Following gene: 21243762

Centisome position: 68.7

GC content: 68.01

Gene sequence:

>1044_bases
GTGACGCCGACGCCGCGGCTGGCCTTGCTGGGGACCGGCACCGTGGGGCGCGCTTTCGTGACGCGCTACACCGCGCTGCA
GCAGCGCCAACTATGCTTGCCGCGCTTCGATTGGCTGGCCAATTCACGTGTTGCGCAGGATTGTGGCGTCAGCGCCGAGC
AGGCCCTGCAGCACGCCAATGCGGCGCCACGCGGGCAGACGGTCTTGCAGCCGTGGGCAGAAACCACCGCGCTGCGTGCC
GGCGATGTGCTGGTGGATGCGACTGCCAGCGATGTCGTTGCCGATTGGCATGTCGAATGGTTGCAGCGCGGCGTGCATGT
GGTGACCGCCAACAAACTGGGCCAGGGCACCGCGTTGTCGCGTGCACAGGCGCTGCACGCGGCCCGCCATGCCAGCGGCG
CGTATTACGGCGATAGCGCCACTGTGGGCGCGGGACTGCCGGTGCTGAGCAGCGTGCGCGCCTTGGTCGCCGGTGGGGAC
CGCATTCATTCGATCGAAGGCGTGTTGTCCGGATCGTTGGCGTGGCTGTTTCATCGTTACGACGGCAGTGGCGCGTTTTC
CGCCTGCGTGCGGGAAGCGATGGTGGCTGGTTATACCGAGCCGGATCCACGCATCGATCTCTCTGGAGAGGATGTGCGGC
GCAAGCTGCTGATCCTGGCGCGTGCCGCGGGGTGGCAGCTGGAGGCCGAGCAGGTGCATGTGGAATCGCTGGTGCCTGCC
ACTATTGCCAGGCTGCCGCTTGCTGAGCTGGATGCGCAGTTGGGCGCGCTGGATGCGGTGGTGGGGACGCGTTGGCAGCA
GGCGCGTGCGGCAGGGCGTTGCCTGCGCTTTGTCGGCCGCGTGGACGCGCATGGAGCGAGCGTGGGCTTGCGCGAACTGG
CGCTGGATCACCCGTTGGCAGGCGGTGCCGGGACCGATAATCGGGTGGCGATCAGCAGCGACCGCTATCGCGCGCAGCCG
CTGCTGATCCAGGGACCAGGCGCCGGGGCCGAAGTGACTGCAGCGGCGCTGCTGGATGATGTGCTGCGGATTGTGGCCGG
CTGA

Upstream 100 bases:

>100_bases
GTCTGGCCCGTGCCGAAGCGACCCTGACTAACGCCAGCCGCAAACAGGTCGACGCGTGAGCAATGTCCTGCCCGTTTCGC
GCCGCGCTCCGGTCGCTGCA

Downstream 100 bases:

>100_bases
TGACGCGACCGGGCTGACGCGGCGCGTTGCCGTGCGCCTTGCGGGGGGTGCGGATGCGTGCGCGCGATCGACGCTGCGCG
TGCCGACGCGCTGGAACCTC

Product: homoserine dehydrogenase

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTPTPRLALLGTGTVGRAFVTRYTALQQRQLCLPRFDWLANSRVAQDCGVSAEQALQHANAAPRGQTVLQPWAETTALRA
GDVLVDATASDVVADWHVEWLQRGVHVVTANKLGQGTALSRAQALHAARHASGAYYGDSATVGAGLPVLSSVRALVAGGD
RIHSIEGVLSGSLAWLFHRYDGSGAFSACVREAMVAGYTEPDPRIDLSGEDVRRKLLILARAAGWQLEAEQVHVESLVPA
TIARLPLAELDAQLGALDAVVGTRWQQARAAGRCLRFVGRVDAHGASVGLRELALDHPLAGGAGTDNRVAISSDRYRAQP
LLIQGPGAGAEVTAAALLDDVLRIVAG

Sequences:

>Translated_347_residues
MTPTPRLALLGTGTVGRAFVTRYTALQQRQLCLPRFDWLANSRVAQDCGVSAEQALQHANAAPRGQTVLQPWAETTALRA
GDVLVDATASDVVADWHVEWLQRGVHVVTANKLGQGTALSRAQALHAARHASGAYYGDSATVGAGLPVLSSVRALVAGGD
RIHSIEGVLSGSLAWLFHRYDGSGAFSACVREAMVAGYTEPDPRIDLSGEDVRRKLLILARAAGWQLEAEQVHVESLVPA
TIARLPLAELDAQLGALDAVVGTRWQQARAAGRCLRFVGRVDAHGASVGLRELALDHPLAGGAGTDNRVAISSDRYRAQP
LLIQGPGAGAEVTAAALLDDVLRIVAG
>Mature_346_residues
TPTPRLALLGTGTVGRAFVTRYTALQQRQLCLPRFDWLANSRVAQDCGVSAEQALQHANAAPRGQTVLQPWAETTALRAG
DVLVDATASDVVADWHVEWLQRGVHVVTANKLGQGTALSRAQALHAARHASGAYYGDSATVGAGLPVLSSVRALVAGGDR
IHSIEGVLSGSLAWLFHRYDGSGAFSACVREAMVAGYTEPDPRIDLSGEDVRRKLLILARAAGWQLEAEQVHVESLVPAT
IARLPLAELDAQLGALDAVVGTRWQQARAAGRCLRFVGRVDAHGASVGLRELALDHPLAGGAGTDNRVAISSDRYRAQPL
LIQGPGAGAEVTAAALLDDVLRIVAG

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0460

COG function: function code E; Homoserine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790376, Length=263, Percent_Identity=45.2471482889734, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI1786183, Length=266, Percent_Identity=39.4736842105263, Blast_Score=184, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6322599, Length=371, Percent_Identity=32.0754716981132, Blast_Score=174, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 36538; Mature: 36407

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS01042 HOMOSER_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTPRLALLGTGTVGRAFVTRYTALQQRQLCLPRFDWLANSRVAQDCGVSAEQALQHAN
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHCCCCHHHHHHHCC
AAPRGQTVLQPWAETTALRAGDVLVDATASDVVADWHVEWLQRGVHVVTANKLGQGTALS
CCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
RAQALHAARHASGAYYGDSATVGAGLPVLSSVRALVAGGDRIHSIEGVLSGSLAWLFHRY
HHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEEEE
DGSGAFSACVREAMVAGYTEPDPRIDLSGEDVRRKLLILARAAGWQLEAEQVHVESLVPA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
TIARLPLAELDAQLGALDAVVGTRWQQARAAGRCLRFVGRVDAHGASVGLRELALDHPLA
HHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
GGAGTDNRVAISSDRYRAQPLLIQGPGAGAEVTAAALLDDVLRIVAG
CCCCCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPTPRLALLGTGTVGRAFVTRYTALQQRQLCLPRFDWLANSRVAQDCGVSAEQALQHAN
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHCCCCHHHHHHHCC
AAPRGQTVLQPWAETTALRAGDVLVDATASDVVADWHVEWLQRGVHVVTANKLGQGTALS
CCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
RAQALHAARHASGAYYGDSATVGAGLPVLSSVRALVAGGDRIHSIEGVLSGSLAWLFHRY
HHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEEEE
DGSGAFSACVREAMVAGYTEPDPRIDLSGEDVRRKLLILARAAGWQLEAEQVHVESLVPA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
TIARLPLAELDAQLGALDAVVGTRWQQARAAGRCLRFVGRVDAHGASVGLRELALDHPLA
HHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
GGAGTDNRVAISSDRYRAQPLLIQGPGAGAEVTAAALLDDVLRIVAG
CCCCCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503 [H]