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The map label for this gene is pyrF

Identifier: 21244993

GI number: 21244993

Start: 5051681

End: 5052412

Strand: Reverse

Name: pyrF

Synonym: XAC4276

Alternate gene names: 21244993

Gene position: 5052412-5051681 (Counterclockwise)

Preceding gene: 21244994

Following gene: 77748772

Centisome position: 97.62

GC content: 68.99

Gene sequence:

>732_bases
ATGAGCCGCGCGCCATTGCCGCTGGCCGCACATGAGCGGCTGATCTTCGCGCTGGATGTGCCCAGCCACGACCAAGCAAT
CGCCTGGGTGGACCGGCTCGGCGATTCGGTGTCGTTCTACAAGATCGGCATGGAGCTGCTGGCATCGGGCGAGTACTTCC
ACGTGCTCGATGCGCTGGCCAAGCGCGACAAGCGCGTGTTCGTGGACCTCAAGTTCTTCGACATTCCTGCCACCGTGGCC
GGCACGATTCGCCGCCTGGCGCAGTGGCCGGTGAGCTATTGCACCGTGCACGGCTGGCACGCCGGCATGCTGCAGGCTGC
CGCCGAAGCCAACCACGGCGACATGCGCCTGCTGGCGGTCACGGTGCTGACGTCGATGGGCCGCCCGGACCTGGTGGCAA
TGGGCATCGACCGCGAACCGGTGGACGTGGTGGTGGAGCGCGCGCTGGCCGCACGGGCCGCCGGGATCGATGGCGTGATC
GCTTCCGGGCAGGAAGCCGGCCCGATCCGCCGTGCCACCGGTCCGGACTTTTCGATCGTCTGCCCCGGCATCCGCCCCGG
CGGCCCGGTCGGCGACGACCAGCAACGCACGGTCGGCGTGGCTCAGGCATTTATCGACGGCGCCGACGCGATCGTCGTTG
GCCGCCCGATCCGCCTGGCGAGCGATCCTGCAGCGGCGGCTGACGCCATCCAGGCGGAGATCCGCGCGGCACTGATGCAG
AACGGAGACTAA

Upstream 100 bases:

>100_bases
CCAGCTACGGCGGCTGGGAGCCGGCGCAGTTCAATAACGACTACGCGCAACCCTGGCAAACGCGCCACGACGCCAAGCGC
GCTGCGCTGGAGGTGGCCCG

Downstream 100 bases:

>100_bases
AACAGCCGTTGGCTCCAAAACAATCAATGGCTTATGCGACAACAATTAAAGCATTGCTTTAATGTGAAAGCCTCGTTAAC
ATGCCGGCGTCCGATGTCAG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSRAPLPLAAHERLIFALDVPSHDQAIAWVDRLGDSVSFYKIGMELLASGEYFHVLDALAKRDKRVFVDLKFFDIPATVA
GTIRRLAQWPVSYCTVHGWHAGMLQAAAEANHGDMRLLAVTVLTSMGRPDLVAMGIDREPVDVVVERALAARAAGIDGVI
ASGQEAGPIRRATGPDFSIVCPGIRPGGPVGDDQQRTVGVAQAFIDGADAIVVGRPIRLASDPAAAADAIQAEIRAALMQ
NGD

Sequences:

>Translated_243_residues
MSRAPLPLAAHERLIFALDVPSHDQAIAWVDRLGDSVSFYKIGMELLASGEYFHVLDALAKRDKRVFVDLKFFDIPATVA
GTIRRLAQWPVSYCTVHGWHAGMLQAAAEANHGDMRLLAVTVLTSMGRPDLVAMGIDREPVDVVVERALAARAAGIDGVI
ASGQEAGPIRRATGPDFSIVCPGIRPGGPVGDDQQRTVGVAQAFIDGADAIVVGRPIRLASDPAAAADAIQAEIRAALMQ
NGD
>Mature_242_residues
SRAPLPLAAHERLIFALDVPSHDQAIAWVDRLGDSVSFYKIGMELLASGEYFHVLDALAKRDKRVFVDLKFFDIPATVAG
TIRRLAQWPVSYCTVHGWHAGMLQAAAEANHGDMRLLAVTVLTSMGRPDLVAMGIDREPVDVVVERALAARAAGIDGVIA
SGQEAGPIRRATGPDFSIVCPGIRPGGPVGDDQQRTVGVAQAFIDGADAIVVGRPIRLASDPAAAADAIQAEIRAALMQN
GD

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=228, Percent_Identity=37.280701754386, Blast_Score=120, Evalue=7e-29,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_XANAC (Q8PER4)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644575.1
- ProteinModelPortal:   Q8PER4
- SMR:   Q8PER4
- GeneID:   1158347
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC4276
- NMPDR:   fig|190486.1.peg.4219
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- BioCyc:   XAXO190486:XAC4276-MONOMER
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25780; Mature: 25649

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 71-71 BINDING 19-19 BINDING 41-41 BINDING 124-124 BINDING 185-185 BINDING 194-194 BINDING 214-214 BINDING 215-215

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAPLPLAAHERLIFALDVPSHDQAIAWVDRLGDSVSFYKIGMELLASGEYFHVLDALA
CCCCCCCHHCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHH
KRDKRVFVDLKFFDIPATVAGTIRRLAQWPVSYCTVHGWHAGMLQAAAEANHGDMRLLAV
HCCCEEEEEEEEECCCHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHCCCCCCHHHHHH
TVLTSMGRPDLVAMGIDREPVDVVVERALAARAAGIDGVIASGQEAGPIRRATGPDFSIV
HHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEE
CPGIRPGGPVGDDQQRTVGVAQAFIDGADAIVVGRPIRLASDPAAAADAIQAEIRAALMQ
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHC
NGD
CCC
>Mature Secondary Structure 
SRAPLPLAAHERLIFALDVPSHDQAIAWVDRLGDSVSFYKIGMELLASGEYFHVLDALA
CCCCCCHHCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHH
KRDKRVFVDLKFFDIPATVAGTIRRLAQWPVSYCTVHGWHAGMLQAAAEANHGDMRLLAV
HCCCEEEEEEEEECCCHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHCCCCCCHHHHHH
TVLTSMGRPDLVAMGIDREPVDVVVERALAARAAGIDGVIASGQEAGPIRRATGPDFSIV
HHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEE
CPGIRPGGPVGDDQQRTVGVAQAFIDGADAIVVGRPIRLASDPAAAADAIQAEIRAALMQ
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHC
NGD
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217