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The map label for this gene is ttcA

Identifier: 21244941

GI number: 21244941

Start: 4983040

End: 4983957

Strand: Reverse

Name: ttcA

Synonym: XAC4224

Alternate gene names: 21244941

Gene position: 4983957-4983040 (Counterclockwise)

Preceding gene: 77748766

Following gene: 21244940

Centisome position: 96.3

GC content: 68.41

Gene sequence:

>918_bases
ATGACCGCTGTCCTGCCGCTGCCGCAACCCCTGGCCGACCCCGCGCCGCGCGACCCGCGCCAGCGGCTGCAGCGTGAGCA
GCTGCGCCTGGGCAAGCGCCTGCAGCGCCAGGTGGGCCAGGCGATTGCCGACTTCGGCATGATCGCGCCCGGCGACAAGA
TCATGGTCTGCCTGTCCGGTGGCAAGGACAGCTACACCTTGCTGGACATGCTGCTGCAGTTGCAGCGCAAGGCGCCGGTG
CCGTTCAGCCTGGTGGCGGTCAATCTGGACCAGAAGCAGCCCGACTTCCCCGCCCATGTGCTGCCGGCCTACCTGCGCGG
GCTGGGTGTGCCGTTCGCTATCGTCGAGCAGGACACCTATTCGGTGGTCAGCCGGGTGATTCCGGCCGGCAAGACGATGT
GTTCGCTGTGCTCGCGGCTGCGGCGCGGCGCGCTGTATGCCTATGCCCAGACGCATGGCGTGACCAAGATCGCGCTCGGC
CACCATCGCGACGACATCGTGGCCACCTTCTTCATGAACCTGTTTCACCACGCGCGGCTGGCGGCGATGGCGCCCAAGCT
GCGCAGCGACGACGGCGCGCATGTGGTGATCCGCCCGCTGGCCTACGTGCGCGAGGCCGACATCGCGGCGTACGCGCAGG
CGCGGCAGTTTCCGATCATTCCGTGCAACTTGTGCGGCAGCCAGGAAAACCTGCAGCGCCAGCAGGTCGGCAAGCTGCTG
CAGCAATGGGACCGCGAGTCCCCCGGGCGCGTGGAACAGATTGCACGCGCCTTGGGCGATGTTCGCCCGGAGCAATTGGC
CGACCGCACGTTGTTCGATTTCCTGGCGCTGGGCCGCAGCGGCGATGCGCCGAGCGGTCTTGATCCCGATCCCCGCGCGT
GGCTGTCCGCCGGCCACGCCACACACGACAGCGACTGA

Upstream 100 bases:

>100_bases
TTCACGGGGAAGGTGCCCGAAGGGCGGATGGGCGAGGGGCCGCGCCGTCACACCGCACGCCCCTCATCGCACCGGCCACG
GCAGCTACAATGGCCGGCTT

Downstream 100 bases:

>100_bases
CGCGGCCGATCCGGACGCCGGCAGTCTTTCCACCCTCCACATCTTTCTTTATTCGGTCCAACGCATGTTCTTTCGCAATC
TCACCCTGTTCCGTTTTCCC

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTAVLPLPQPLADPAPRDPRQRLQREQLRLGKRLQRQVGQAIADFGMIAPGDKIMVCLSGGKDSYTLLDMLLQLQRKAPV
PFSLVAVNLDQKQPDFPAHVLPAYLRGLGVPFAIVEQDTYSVVSRVIPAGKTMCSLCSRLRRGALYAYAQTHGVTKIALG
HHRDDIVATFFMNLFHHARLAAMAPKLRSDDGAHVVIRPLAYVREADIAAYAQARQFPIIPCNLCGSQENLQRQQVGKLL
QQWDRESPGRVEQIARALGDVRPEQLADRTLFDFLALGRSGDAPSGLDPDPRAWLSAGHATHDSD

Sequences:

>Translated_305_residues
MTAVLPLPQPLADPAPRDPRQRLQREQLRLGKRLQRQVGQAIADFGMIAPGDKIMVCLSGGKDSYTLLDMLLQLQRKAPV
PFSLVAVNLDQKQPDFPAHVLPAYLRGLGVPFAIVEQDTYSVVSRVIPAGKTMCSLCSRLRRGALYAYAQTHGVTKIALG
HHRDDIVATFFMNLFHHARLAAMAPKLRSDDGAHVVIRPLAYVREADIAAYAQARQFPIIPCNLCGSQENLQRQQVGKLL
QQWDRESPGRVEQIARALGDVRPEQLADRTLFDFLALGRSGDAPSGLDPDPRAWLSAGHATHDSD
>Mature_304_residues
TAVLPLPQPLADPAPRDPRQRLQREQLRLGKRLQRQVGQAIADFGMIAPGDKIMVCLSGGKDSYTLLDMLLQLQRKAPVP
FSLVAVNLDQKQPDFPAHVLPAYLRGLGVPFAIVEQDTYSVVSRVIPAGKTMCSLCSRLRRGALYAYAQTHGVTKIALGH
HRDDIVATFFMNLFHHARLAAMAPKLRSDDGAHVVIRPLAYVREADIAAYAQARQFPIIPCNLCGSQENLQRQQVGKLLQ
QWDRESPGRVEQIARALGDVRPEQLADRTLFDFLALGRSGDAPSGLDPDPRAWLSAGHATHDSD

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=275, Percent_Identity=58.1818181818182, Blast_Score=340, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI193211090, Length=260, Percent_Identity=33.0769230769231, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17540090, Length=199, Percent_Identity=26.6331658291457, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_XANAC (Q8PEW6)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644523.1
- ProteinModelPortal:   Q8PEW6
- GeneID:   1158295
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC4224
- NMPDR:   fig|190486.1.peg.4167
- HOGENOM:   HBG347038
- OMA:   FPHAREE
- ProtClustDB:   PRK10696
- BioCyc:   XAXO190486:XAC4224-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 33645; Mature: 33514

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVLPLPQPLADPAPRDPRQRLQREQLRLGKRLQRQVGQAIADFGMIAPGDKIMVCLSG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
GKDSYTLLDMLLQLQRKAPVPFSLVAVNLDQKQPDFPAHVLPAYLRGLGVPFAIVEQDTY
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCHHH
SVVSRVIPAGKTMCSLCSRLRRGALYAYAQTHGVTKIALGHHRDDIVATFFMNLFHHARL
HHHHHHHCCCHHHHHHHHHHHHHHEEEHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHH
AAMAPKLRSDDGAHVVIRPLAYVREADIAAYAQARQFPIIPCNLCGSQENLQRQQVGKLL
HHHCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCHHHHHHHHHHHHH
QQWDRESPGRVEQIARALGDVRPEQLADRTLFDFLALGRSGDAPSGLDPDPRAWLSAGHA
HHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCC
THDSD
CCCCC
>Mature Secondary Structure 
TAVLPLPQPLADPAPRDPRQRLQREQLRLGKRLQRQVGQAIADFGMIAPGDKIMVCLSG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
GKDSYTLLDMLLQLQRKAPVPFSLVAVNLDQKQPDFPAHVLPAYLRGLGVPFAIVEQDTY
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCHHH
SVVSRVIPAGKTMCSLCSRLRRGALYAYAQTHGVTKIALGHHRDDIVATFFMNLFHHARL
HHHHHHHCCCHHHHHHHHHHHHHHEEEHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHH
AAMAPKLRSDDGAHVVIRPLAYVREADIAAYAQARQFPIIPCNLCGSQENLQRQQVGKLL
HHHCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEHHCCCCHHHHHHHHHHHHH
QQWDRESPGRVEQIARALGDVRPEQLADRTLFDFLALGRSGDAPSGLDPDPRAWLSAGHA
HHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCC
THDSD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217