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The map label for this gene is dusA

Identifier: 21244742

GI number: 21244742

Start: 4716300

End: 4717304

Strand: Reverse

Name: dusA

Synonym: XAC4025

Alternate gene names: 21244742

Gene position: 4717304-4716300 (Counterclockwise)

Preceding gene: 21244743

Following gene: 21244741

Centisome position: 91.15

GC content: 67.36

Gene sequence:

>1005_bases
ATGACCGCTTCCACCGATCGTTACGCCGCCTCCCTGCGCCTGTCCGTTGCCCCGATGATGGATTGGACCGACCGCCATTG
CCGGGTCTTCCACCGCCTGTTGGCGCCGTCGGCACGCCTCTACACCGAGATGGTGCACGCCAACGCGGTGATCCACGGCG
ATCGCGCGCGCTTGATCGGCTTCGATCCGGTGGAACATCCGCTGGCGCTGCAACTGGGCGGCAGCGATCCTGCCTTGCTC
GCGCAGGCGGCGGCAATCGCGCAGGAATGGGGCTTTGATGAGATCAATCTCAATTGCGGTTGCCCGTCCGACCGCGTGCA
GGCCGGGCGTTTTGGCGCCTGCCTGATGCGTGAGCCGGCGCTGGTGGCCGAGTGCGTTGCGGCCATGTGCGCGGCCACCC
ACTTGCCGATCACGGTCAAGTGCCGCCTGGGCGTGGACGACGATGACGACTACGCGCTGTTCGCCCGCTTTGTCGACCAG
GTGGTCGCGGCCGGCGCCGCCATGGTGGTGGTGCACGCCCGTAATGCCTGGCTCAAGGGCTTGTCGCCGAAGGAAAATCG
CGAAGTGCCGCCGCTGCGGTACGACTGGGCCTACCGGCTCAAGCAGGAGCGGCCCGGTCTGCCGGTCGTGCTCAACGGCG
GCATTGTGGCCGTCGAGCCGGCGATAGCGCATCTGCAGCACGCCGACGGCGTCATGCTCGGCCGCGCCGCCTACCACGAT
CCGTACGTCCTGCACTGTCTGGATGCAGCCTTGAACCAGCGGCCCGAGCAACCGCGTGAGTCCTTGCTGCGCGCCTATCA
GCCGTATGTGGAATCACAGCTGGCACAGGGTCTGGGCTTGAAGCACATGACCCGGCACGTGCTGGGCCTGTTCCACGGCC
AGCCTGGAGGCAGGGCGTTCCGGCAGGTCCTGAGCGAGGGCGCCCATCGTCCGGGTGCCGGCTGGGAATTGGTCGAGCAG
GCATTGGAGCGCACCGATACGCGGTCCTGGCGAGTCGTTGCCTGA

Upstream 100 bases:

>100_bases
CCCGCATCCCATGCCGCACTGCCGCAGATTCATCGGGGCCCGTGTAAGCTGTTGATCCTGCTTCGCATTCCCATGGCCGC
TTGCTGCGGCTACCTCGTCG

Downstream 100 bases:

>100_bases
CTTTGGCCCACCGCTTGCATTCCTGAACAGGCTGCCGGCATATCCGAGTATTTCCGGTCGCTTCTGCGGCAGGCGCGTCC
GCAACAACGCGCGCTGGTCG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTASTDRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLIGFDPVEHPLALQLGGSDPALL
AQAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPALVAECVAAMCAATHLPITVKCRLGVDDDDDYALFARFVDQ
VVAAGAAMVVVHARNAWLKGLSPKENREVPPLRYDWAYRLKQERPGLPVVLNGGIVAVEPAIAHLQHADGVMLGRAAYHD
PYVLHCLDAALNQRPEQPRESLLRAYQPYVESQLAQGLGLKHMTRHVLGLFHGQPGGRAFRQVLSEGAHRPGAGWELVEQ
ALERTDTRSWRVVA

Sequences:

>Translated_334_residues
MTASTDRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLIGFDPVEHPLALQLGGSDPALL
AQAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPALVAECVAAMCAATHLPITVKCRLGVDDDDDYALFARFVDQ
VVAAGAAMVVVHARNAWLKGLSPKENREVPPLRYDWAYRLKQERPGLPVVLNGGIVAVEPAIAHLQHADGVMLGRAAYHD
PYVLHCLDAALNQRPEQPRESLLRAYQPYVESQLAQGLGLKHMTRHVLGLFHGQPGGRAFRQVLSEGAHRPGAGWELVEQ
ALERTDTRSWRVVA
>Mature_333_residues
TASTDRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLIGFDPVEHPLALQLGGSDPALLA
QAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPALVAECVAAMCAATHLPITVKCRLGVDDDDDYALFARFVDQV
VAAGAAMVVVHARNAWLKGLSPKENREVPPLRYDWAYRLKQERPGLPVVLNGGIVAVEPAIAHLQHADGVMLGRAAYHDP
YVLHCLDAALNQRPEQPRESLLRAYQPYVESQLAQGLGLKHMTRHVLGLFHGQPGGRAFRQVLSEGAHRPGAGWELVEQA
LERTDTRSWRVVA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily

Homologues:

Organism=Homo sapiens, GI31742496, Length=233, Percent_Identity=28.755364806867, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI40807366, Length=241, Percent_Identity=27.3858921161826, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=53.1645569620253, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1788462, Length=221, Percent_Identity=32.1266968325792, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789660, Length=256, Percent_Identity=28.515625, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=256, Percent_Identity=25.78125, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=167, Percent_Identity=28.7425149700599, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=285, Percent_Identity=24.9122807017544, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24580595, Length=241, Percent_Identity=28.2157676348548, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19920448, Length=241, Percent_Identity=28.2157676348548, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24585320, Length=187, Percent_Identity=29.9465240641711, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSA_XANAC (Q8PFF8)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644324.1
- ProteinModelPortal:   Q8PFF8
- SMR:   Q8PFF8
- GeneID:   1158096
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC4025
- NMPDR:   fig|190486.1.peg.3968
- HOGENOM:   HBG630545
- OMA:   RIGIDEQ
- ProtClustDB:   PRK11815
- BioCyc:   XAXO190486:XAC4025-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621
- TIGRFAMs:   TIGR00742

Pfam domain/function: PF01207 Dus

EC number: 1.-.-.-

Molecular weight: Translated: 36733; Mature: 36601

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASTDRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLIG
CCCCCCHHHHHEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCEEEECCCEEEEE
FDPVEHPLALQLGGSDPALLAQAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPA
CCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCHHHHCCHH
LVAECVAAMCAATHLPITVKCRLGVDDDDDYALFARFVDQVVAAGAAMVVVHARNAWLKG
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHCC
LSPKENREVPPLRYDWAYRLKQERPGLPVVLNGGIVAVEPAIAHLQHADGVMLGRAAYHD
CCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCEEEECHHHHHHHHCCCEEECHHHCCC
PYVLHCLDAALNQRPEQPRESLLRAYQPYVESQLAQGLGLKHMTRHVLGLFHGQPGGRAF
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHH
RQVLSEGAHRPGAGWELVEQALERTDTRSWRVVA
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure 
TASTDRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLIG
CCCCCHHHHHEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCEEEECCCEEEEE
FDPVEHPLALQLGGSDPALLAQAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPA
CCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCHHHHCCHH
LVAECVAAMCAATHLPITVKCRLGVDDDDDYALFARFVDQVVAAGAAMVVVHARNAWLKG
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHCC
LSPKENREVPPLRYDWAYRLKQERPGLPVVLNGGIVAVEPAIAHLQHADGVMLGRAAYHD
CCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCEEEECHHHHHHHHCCCEEECHHHCCC
PYVLHCLDAALNQRPEQPRESLLRAYQPYVESQLAQGLGLKHMTRHVLGLFHGQPGGRAF
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHH
RQVLSEGAHRPGAGWELVEQALERTDTRSWRVVA
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217