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The map label for this gene is birA [H]

Identifier: 21244735

GI number: 21244735

Start: 4707904

End: 4708869

Strand: Reverse

Name: birA [H]

Synonym: XAC4018

Alternate gene names: 21244735

Gene position: 4708869-4707904 (Counterclockwise)

Preceding gene: 21244737

Following gene: 21244734

Centisome position: 90.98

GC content: 69.67

Gene sequence:

>966_bases
GTGGATGACCGTACATTGCTGGCCAAACTTGCCGGTGGCCGGCTGTCTGGCGATGCGTTGGCGCGCGATGCGGGGCTGAC
CCGCGCGGCGGTGTGGAAGCGCATCCAGAATCTGCGAGCGGCCGGCGTCGACATCGAGGGACGGGCTGGTGACGGGTACC
GCCTGGCCACGCCGTTGGATCTGCTGGACGCGACGCGCATCCGTGCAGGCATGGCACCCGGTGCGGCAGCTGGGCTGGCG
GCACTGGATGTGGCCTGGACGCTGGACTCCACCAACACCACCTTGCTGGCGCAGCCGGCGCCCGAACAGGGTGTGGCGGT
GCTGCTGGCGGAGCGCCAGACCGGCGGCCGCGGCCGGCGCGGCCGTCAATGGACCTCGCCGCTGGGCGCGCACATTTATC
TGTCGGCGTCGCGCCGTTTCAATGGCGGGCTGGGCCAACTCGCAGGCCTGAGCTTGGCTGTGGGTGTGGCAGTGGCCGAA
GCCTTGCGCGGTTGCGGGTTCGCCGATGTCGGGCTGAAGTGGCCCAACGATCTGCTGGCGCGGCAGCGCAAGCTGGGCGG
CCTGTTGATCGAAGGCGGCGGCGAGATGGCCGGCAACGCGCGTGCAGTGATCGGGCTGGGCTTGAACGTACACATGCCGG
CGAGCGCAGCGGCCAGCATCGACCAGCCGTGGGTCGACCTGGATACCCTGGCTGGCAGCCCGGTGTCGCGCGACCGGGTG
ACGGCAGCGGTGCTGTCCGGGTTGCTGCCGGCACTGGACCTGTTCGAGGCGCAGGGGCTGGCGCCGTTTCTGGGACGCTA
TGCAGACCTGGATGTCTTGAGCGGGCGAGCGGTGCAGGTCGAAGAAGCCGGCAACCGACATACTGGAGTCGCGCTCGGCC
TGGCGGCCGATGGTGCGTTGCGGGTGCAGCTGGGCGAGACGGTGCGCCTGTTCCACTCCGGAGAAGTGAGCGTGAGACCT
GCATGA

Upstream 100 bases:

>100_bases
GCGTTGCCCCGGCGGCACACCGGCTGCGCAGCGCAGGCATTGCTTCCGGGTGCTGCAGCCCGCGACGTCGCCACGTCACG
CCCCATCGCCCGCCGCAGCC

Downstream 100 bases:

>100_bases
GCGAGTGGTTGTTCGACCTGGGAAATTCGCGCTTCAAGTACGCGCCGCTGCACGGCAACCGTGCCGGCCAAGTGCAGGCA
TGGGCGCATGGCGCCGAGGC

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MDDRTLLAKLAGGRLSGDALARDAGLTRAAVWKRIQNLRAAGVDIEGRAGDGYRLATPLDLLDATRIRAGMAPGAAAGLA
ALDVAWTLDSTNTTLLAQPAPEQGVAVLLAERQTGGRGRRGRQWTSPLGAHIYLSASRRFNGGLGQLAGLSLAVGVAVAE
ALRGCGFADVGLKWPNDLLARQRKLGGLLIEGGGEMAGNARAVIGLGLNVHMPASAAASIDQPWVDLDTLAGSPVSRDRV
TAAVLSGLLPALDLFEAQGLAPFLGRYADLDVLSGRAVQVEEAGNRHTGVALGLAADGALRVQLGETVRLFHSGEVSVRP
A

Sequences:

>Translated_321_residues
MDDRTLLAKLAGGRLSGDALARDAGLTRAAVWKRIQNLRAAGVDIEGRAGDGYRLATPLDLLDATRIRAGMAPGAAAGLA
ALDVAWTLDSTNTTLLAQPAPEQGVAVLLAERQTGGRGRRGRQWTSPLGAHIYLSASRRFNGGLGQLAGLSLAVGVAVAE
ALRGCGFADVGLKWPNDLLARQRKLGGLLIEGGGEMAGNARAVIGLGLNVHMPASAAASIDQPWVDLDTLAGSPVSRDRV
TAAVLSGLLPALDLFEAQGLAPFLGRYADLDVLSGRAVQVEEAGNRHTGVALGLAADGALRVQLGETVRLFHSGEVSVRP
A
>Mature_321_residues
MDDRTLLAKLAGGRLSGDALARDAGLTRAAVWKRIQNLRAAGVDIEGRAGDGYRLATPLDLLDATRIRAGMAPGAAAGLA
ALDVAWTLDSTNTTLLAQPAPEQGVAVLLAERQTGGRGRRGRQWTSPLGAHIYLSASRRFNGGLGQLAGLSLAVGVAVAE
ALRGCGFADVGLKWPNDLLARQRKLGGLLIEGGGEMAGNARAVIGLGLNVHMPASAAASIDQPWVDLDTLAGSPVSRDRV
TAAVLSGLLPALDLFEAQGLAPFLGRYADLDVLSGRAVQVEEAGNRHTGVALGLAADGALRVQLGETVRLFHSGEVSVRP
A

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=326, Percent_Identity=38.3435582822086, Blast_Score=197, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 33089; Mature: 33089

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDRTLLAKLAGGRLSGDALARDAGLTRAAVWKRIQNLRAAGVDIEGRAGDGYRLATPLD
CCCHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCCCCEEEECCHH
LLDATRIRAGMAPGAAAGLAALDVAWTLDSTNTTLLAQPAPEQGVAVLLAERQTGGRGRR
HHHHHHHHHCCCCCHHHHHEEEEEEEEECCCCEEEEECCCCCCCEEEEEEECCCCCCCCC
GRQWTSPLGAHIYLSASRRFNGGLGQLAGLSLAVGVAVAEALRGCGFADVGLKWPNDLLA
CCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHH
RQRKLGGLLIEGGGEMAGNARAVIGLGLNVHMPASAAASIDQPWVDLDTLAGSPVSRDRV
HHHHHCCEEEECCCCCCCCCEEEEEECCEEECCCHHHCCCCCCCCCHHHHCCCCCCHHHH
TAAVLSGLLPALDLFEAQGLAPFLGRYADLDVLSGRAVQVEEAGNRHTGVALGLAADGAL
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEECCCCEEEECCCCCCCCCEEEEEECCCEE
RVQLGETVRLFHSGEVSVRPA
EEEECCEEEEEECCCEEEECC
>Mature Secondary Structure
MDDRTLLAKLAGGRLSGDALARDAGLTRAAVWKRIQNLRAAGVDIEGRAGDGYRLATPLD
CCCHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCCCCEEEECCHH
LLDATRIRAGMAPGAAAGLAALDVAWTLDSTNTTLLAQPAPEQGVAVLLAERQTGGRGRR
HHHHHHHHHCCCCCHHHHHEEEEEEEEECCCCEEEEECCCCCCCEEEEEEECCCCCCCCC
GRQWTSPLGAHIYLSASRRFNGGLGQLAGLSLAVGVAVAEALRGCGFADVGLKWPNDLLA
CCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHH
RQRKLGGLLIEGGGEMAGNARAVIGLGLNVHMPASAAASIDQPWVDLDTLAGSPVSRDRV
HHHHHCCEEEECCCCCCCCCEEEEEECCEEECCCHHHCCCCCCCCCHHHHCCCCCCHHHH
TAAVLSGLLPALDLFEAQGLAPFLGRYADLDVLSGRAVQVEEAGNRHTGVALGLAADGAL
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEECCCCEEEECCCCCCCCCEEEEEECCCEE
RVQLGETVRLFHSGEVSVRPA
EEEECCEEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]