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The map label for this gene is pyrE

Identifier: 21244622

GI number: 21244622

Start: 4589498

End: 4590157

Strand: Direct

Name: pyrE

Synonym: XAC3903

Alternate gene names: 21244622

Gene position: 4589498-4590157 (Clockwise)

Preceding gene: 77748746

Following gene: 21244623

Centisome position: 88.68

GC content: 68.94

Gene sequence:

>660_bases
ATGACCGACCACCGCACCCGTTTCCTGCAGCTGGCCCTGGACGCCGATGCCCTGCGCTTTGGCGAGTTCACGCTCAAGTC
CGGGCGGCTCAGCCCGTACTTCTTCAATGCCGGGCGCTTCGATTCGGGGGCCAAGACCGCGCAGTTGGCGCAGTGCTATG
CCGATGCGATCGAAGCGGCCGGGCTGGACTTCGACCTGTTGTTCGGGCCGGCCTACAAGGGCATCCCGTTGGCGACCGCG
CTGGCCTGCGCCTACGCAGGACGCGGGCGCGACCTGCCGCTGGCGTTCAACCGCAAGGAAGCCAAGGACCACGGCGAAGG
CGGCACCCTGATCGGCGCGCCGCTGGCCGGCCGTCAGGTGTTGATCGTCGATGACGTGATCACCGCCGGCACGGCAATCC
GCGAGGCGCTGGCCATCATCCGCGCCGCTGGCGGAACGCCGTCGGGGATCGCGGTGGCGCTGGACCGACAGGAAATCGCG
TCCGATCAGGACCGCCGTTCCGCCGCCCAGGCGGTGGCAGCCGAGGCCGGCATTCCCGTGATCGCGGTGGCCAACCTGGG
CGACCTGCTTGCTTTTGCGGCAGGAAACGCCGACCTTGTGGGCTTCCAGGAACCGCTGCTGGCCTATCGTGGCCGCTACG
GGACCGACACCACAGGCTGA

Upstream 100 bases:

>100_bases
GGGAATGGGGAATCGTAAAGCCTACCCCAGGCGCATGCGGAAGGCTGCCGGCGCACGATGCGTTTACCATTCCCGATTCC
CCATTCCCCATACCCGTCCA

Downstream 100 bases:

>100_bases
CGGCAGGCGACAGGGGGCTGCGATGATGCCAATACAGACACGTTCGGCATTATTCGCCGTTGCGGCGGCTGTCGCTGCGA
CATTGGGCATGGCTGCGCCG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTDHRTRFLQLALDADALRFGEFTLKSGRLSPYFFNAGRFDSGAKTAQLAQCYADAIEAAGLDFDLLFGPAYKGIPLATA
LACAYAGRGRDLPLAFNRKEAKDHGEGGTLIGAPLAGRQVLIVDDVITAGTAIREALAIIRAAGGTPSGIAVALDRQEIA
SDQDRRSAAQAVAAEAGIPVIAVANLGDLLAFAAGNADLVGFQEPLLAYRGRYGTDTTG

Sequences:

>Translated_219_residues
MTDHRTRFLQLALDADALRFGEFTLKSGRLSPYFFNAGRFDSGAKTAQLAQCYADAIEAAGLDFDLLFGPAYKGIPLATA
LACAYAGRGRDLPLAFNRKEAKDHGEGGTLIGAPLAGRQVLIVDDVITAGTAIREALAIIRAAGGTPSGIAVALDRQEIA
SDQDRRSAAQAVAAEAGIPVIAVANLGDLLAFAAGNADLVGFQEPLLAYRGRYGTDTTG
>Mature_218_residues
TDHRTRFLQLALDADALRFGEFTLKSGRLSPYFFNAGRFDSGAKTAQLAQCYADAIEAAGLDFDLLFGPAYKGIPLATAL
ACAYAGRGRDLPLAFNRKEAKDHGEGGTLIGAPLAGRQVLIVDDVITAGTAIREALAIIRAAGGTPSGIAVALDRQEIAS
DQDRRSAAQAVAAEAGIPVIAVANLGDLLAFAAGNADLVGFQEPLLAYRGRYGTDTTG

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=151, Percent_Identity=30.4635761589404, Blast_Score=81, Evalue=8e-16,
Organism=Escherichia coli, GI1790073, Length=216, Percent_Identity=50.462962962963, Blast_Score=205, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17508631, Length=156, Percent_Identity=32.6923076923077, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17554964, Length=155, Percent_Identity=25.1612903225806, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323530, Length=201, Percent_Identity=49.2537313432836, Blast_Score=199, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6323927, Length=220, Percent_Identity=47.2727272727273, Blast_Score=191, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_XANAC (Q8PFS5)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644204.1
- ProteinModelPortal:   Q8PFS5
- SMR:   Q8PFS5
- GeneID:   1157974
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3903
- NMPDR:   fig|190486.1.peg.3848
- HOGENOM:   HBG404341
- OMA:   FAFNRKE
- ProtClustDB:   PRK00455
- BioCyc:   XAXO190486:XAC3903-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 22814; Mature: 22683

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 26-26 BINDING 98-98 BINDING 99-99 BINDING 102-102 BINDING 104-104 BINDING 128-128 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHRTRFLQLALDADALRFGEFTLKSGRLSPYFFNAGRFDSGAKTAQLAQCYADAIEAA
CCCCHHEEEEEEECCCHHHHCCEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHC
GLDFDLLFGPAYKGIPLATALACAYAGRGRDLPLAFNRKEAKDHGEGGTLIGAPLAGRQV
CCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEECCCHHHCCCCCCEEEECCCCCCEE
LIVDDVITAGTAIREALAIIRAAGGTPSGIAVALDRQEIASDQDRRSAAQAVAAEAGIPV
EEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHHHCCCCE
IAVANLGDLLAFAAGNADLVGFQEPLLAYRGRYGTDTTG
EEECCHHHHHHHHCCCCCEECCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TDHRTRFLQLALDADALRFGEFTLKSGRLSPYFFNAGRFDSGAKTAQLAQCYADAIEAA
CCCHHEEEEEEECCCHHHHCCEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHC
GLDFDLLFGPAYKGIPLATALACAYAGRGRDLPLAFNRKEAKDHGEGGTLIGAPLAGRQV
CCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEECCCHHHCCCCCCEEEECCCCCCEE
LIVDDVITAGTAIREALAIIRAAGGTPSGIAVALDRQEIASDQDRRSAAQAVAAEAGIPV
EEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHHHCCCCE
IAVANLGDLLAFAAGNADLVGFQEPLLAYRGRYGTDTTG
EEECCHHHHHHHHCCCCCEECCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217