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The map label for this gene is amiD [H]

Identifier: 21244584

GI number: 21244584

Start: 4542779

End: 4543585

Strand: Reverse

Name: amiD [H]

Synonym: XAC3860

Alternate gene names: 21244584

Gene position: 4543585-4542779 (Counterclockwise)

Preceding gene: 21244591

Following gene: 77748745

Centisome position: 87.79

GC content: 67.16

Gene sequence:

>807_bases
ATGCTGCCCAGCTGTCCTCTCTCGTCACCGGCCTGGCTGCGTACTGCCGTTTGCCTGGTCGCGCTGTCGCTGGGTGCCTG
TGCGCACGCACCGCAGCGCAATCCGCTGGCGCAGTGGGTGCCATCGCCCAATTACGACACGCGGCGACCGATCCTGATCG
TGTTGCACTTCACCGACCAGCATTCAGTCCGGCAAAGCCTGAGTACCCTGCGCGGCCGCAACAGCGGTGGTCGCGTCAGT
GCGCACTACCTGATCGGCGAAGACGGGCAGCGCTACCAATTGGTCAGCGATGGGCAGCGCGCCTGGCACGGCGGGGCCGG
TCGCTGGGGCACCATCACCGACATCAATTCGGCCTCGATCGGCATCGAACTGGACAACGACGGCAGCGAGCCGTTCGCGC
CGGCGCAGATCGACAGCCTGCTGGTGTTGCTGGACGATCTGTGTCAACGCCTGCGTATCCCCCGCACGCAGATCGTTGGC
CACGAAGACGTGGCCCCGACGCGCAAGAACGACCCTGGCCCGCTCTTCCCCTGGAAGCGCCTGGCCGACGCCGGTTTCGG
CCGCTGGCCCGCTGCGGACGCGCCCGCAGCCCCGGAAGATTTCGACCCATGGCAGGCGCTTGCCCTGATCGGCTACAGCA
TCGACGATCCCGCCGCCGCCCTGCAGGCGTTTCACCACCACTATCGCGGCACCAGCGCCACCACCCTGGATGCCGAAGAC
CTGCGCATCCTGTCTGCGTTGACCAACCACGCGAGACCTTCCAGCCTGCGGCCGGCGCTTGAGCCAACTGAAGGCGACAA
CCGCTAG

Upstream 100 bases:

>100_bases
GCACCGCGCTGCCGACGCTCCCACCTGCAGACGGCCGCGCAAGCACAACTCGGCGGATAATGCGACGGCGCAGGTCGTCC
GGCCTTCCCAGGAGCAATCC

Downstream 100 bases:

>100_bases
GCCCACAGGGATCGCGCCATGCTGTAGGCGGTGGCGAAAGACGCGCGGCTTGATCTGCCGCGCAAACGCGGCATCTTGCA
AGGGAACGGACATGACGGAC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MLPSCPLSSPAWLRTAVCLVALSLGACAHAPQRNPLAQWVPSPNYDTRRPILIVLHFTDQHSVRQSLSTLRGRNSGGRVS
AHYLIGEDGQRYQLVSDGQRAWHGGAGRWGTITDINSASIGIELDNDGSEPFAPAQIDSLLVLLDDLCQRLRIPRTQIVG
HEDVAPTRKNDPGPLFPWKRLADAGFGRWPAADAPAAPEDFDPWQALALIGYSIDDPAAALQAFHHHYRGTSATTLDAED
LRILSALTNHARPSSLRPALEPTEGDNR

Sequences:

>Translated_268_residues
MLPSCPLSSPAWLRTAVCLVALSLGACAHAPQRNPLAQWVPSPNYDTRRPILIVLHFTDQHSVRQSLSTLRGRNSGGRVS
AHYLIGEDGQRYQLVSDGQRAWHGGAGRWGTITDINSASIGIELDNDGSEPFAPAQIDSLLVLLDDLCQRLRIPRTQIVG
HEDVAPTRKNDPGPLFPWKRLADAGFGRWPAADAPAAPEDFDPWQALALIGYSIDDPAAALQAFHHHYRGTSATTLDAED
LRILSALTNHARPSSLRPALEPTEGDNR
>Mature_268_residues
MLPSCPLSSPAWLRTAVCLVALSLGACAHAPQRNPLAQWVPSPNYDTRRPILIVLHFTDQHSVRQSLSTLRGRNSGGRVS
AHYLIGEDGQRYQLVSDGQRAWHGGAGRWGTITDINSASIGIELDNDGSEPFAPAQIDSLLVLLDDLCQRLRIPRTQIVG
HEDVAPTRKNDPGPLFPWKRLADAGFGRWPAADAPAAPEDFDPWQALALIGYSIDDPAAALQAFHHHYRGTSATTLDAED
LRILSALTNHARPSSLRPALEPTEGDNR

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=232, Percent_Identity=36.6379310344828, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1786300, Length=166, Percent_Identity=33.1325301204819, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 29204; Mature: 29204

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSCPLSSPAWLRTAVCLVALSLGACAHAPQRNPLAQWVPSPNYDTRRPILIVLHFTDQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCEEEEEEECCC
HSVRQSLSTLRGRNSGGRVSAHYLIGEDGQRYQLVSDGQRAWHGGAGRWGTITDINSASI
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCHHHCCCCCCCCCEEECCCCEE
GIELDNDGSEPFAPAQIDSLLVLLDDLCQRLRIPRTQIVGHEDVAPTRKNDPGPLFPWKR
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCHHH
LADAGFGRWPAADAPAAPEDFDPWQALALIGYSIDDPAAALQAFHHHYRGTSATTLDAED
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECHHH
LRILSALTNHARPSSLRPALEPTEGDNR
HHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLPSCPLSSPAWLRTAVCLVALSLGACAHAPQRNPLAQWVPSPNYDTRRPILIVLHFTDQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCEEEEEEECCC
HSVRQSLSTLRGRNSGGRVSAHYLIGEDGQRYQLVSDGQRAWHGGAGRWGTITDINSASI
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCHHHCCCCCCCCCEEECCCCEE
GIELDNDGSEPFAPAQIDSLLVLLDDLCQRLRIPRTQIVGHEDVAPTRKNDPGPLFPWKR
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCHHH
LADAGFGRWPAADAPAAPEDFDPWQALALIGYSIDDPAAALQAFHHHYRGTSATTLDAED
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECHHH
LRILSALTNHARPSSLRPALEPTEGDNR
HHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]