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The map label for this gene is queF

Identifier: 21244570

GI number: 21244570

Start: 4524883

End: 4525698

Strand: Reverse

Name: queF

Synonym: XAC3846

Alternate gene names: 21244570

Gene position: 4525698-4524883 (Counterclockwise)

Preceding gene: 21244571

Following gene: 21244569

Centisome position: 87.44

GC content: 64.22

Gene sequence:

>816_bases
ATGAATACCCCAGAAGATTCCACCCTCGGTCGTGAGGTCGCCTATCCGAGCGGCTACGACCCGTCGTTGCTGTTTCCGAT
TCCGCGTGCGGCCGGGCGCCAGGCCATCGGGCTGACTGGTGACCTGCCGTTTATCGGCCGCGATCGCTGGCACGCCTACG
AACTCAGCTGGCTCGATGCACAGGGCAAGCCCTGCGTGGCCACCGCCACCTTGCACGTGCCCTGCGATTCGCCCTCGTTG
ATCGAATCCAAGTCGCTCAAGCTGTATCTCAACTCGCTCAACGCCACCCGCTTCAACAGCGCCGAAGCGGTGCGCACGCG
GATTGCGACCGACCTGTCCACGCGCGCCGGTGCCGATGTGGCGGTGGAGTTCGGCCTGCCGCCGATCGATGCGGTGGGCG
AGGGCGAATCCATTGATGCACTGGATCTCAGCATCGACGATTACGGCCCGCCGAACGCGGCCTATCTCTGCGCGCATGCG
CAGCCAGTGGTGGAAGAAGTGCTGACCTCGGCCTTGCTCAAATCCAATTGCCCGGTGACTGGCCAGCCGGACTGGGCCAG
CGTCACCTTGCGTTACCGCGGCGCACCGATCGATCGCGAGGGATTGCTGCGTTATCTGGTGAGTTTCCGCGACCATGCCG
AGTTTCATGAGCAATGCGTCGAGCGCATCTTCAACGACGTGCTGACGCAGTGCGCGCCGCAGTGGCTGGTGGTGGAAGCG
CGCTACACACGTCGTGGCGGCCTGGACATCAATCCGCTGCGCAGCTCGGCCAGTGTCCCGACGCCGCTGTCGATTTTCCG
CGACCTGCGTCAGTAG

Upstream 100 bases:

>100_bases
TGGCTCGATTCTGGAGCGGCGATCTTCGGGTTTTCCATTCGCTCTCTGTCACGCGTTGCCATACACCTCACGGCCAGCCC
GTGCGGCCTAAAATGGCGAT

Downstream 100 bases:

>100_bases
ATCGCGCTCATGCGGGATCGCGCGGGCTTCACGGCGGCGCGATCCATGCTTAACAGCGCCCCGCGCACCCTGCTGACACC
GCGATGCACAGGGATTGCAG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGRQAIGLTGDLPFIGRDRWHAYELSWLDAQGKPCVATATLHVPCDSPSL
IESKSLKLYLNSLNATRFNSAEAVRTRIATDLSTRAGADVAVEFGLPPIDAVGEGESIDALDLSIDDYGPPNAAYLCAHA
QPVVEEVLTSALLKSNCPVTGQPDWASVTLRYRGAPIDREGLLRYLVSFRDHAEFHEQCVERIFNDVLTQCAPQWLVVEA
RYTRRGGLDINPLRSSASVPTPLSIFRDLRQ

Sequences:

>Translated_271_residues
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGRQAIGLTGDLPFIGRDRWHAYELSWLDAQGKPCVATATLHVPCDSPSL
IESKSLKLYLNSLNATRFNSAEAVRTRIATDLSTRAGADVAVEFGLPPIDAVGEGESIDALDLSIDDYGPPNAAYLCAHA
QPVVEEVLTSALLKSNCPVTGQPDWASVTLRYRGAPIDREGLLRYLVSFRDHAEFHEQCVERIFNDVLTQCAPQWLVVEA
RYTRRGGLDINPLRSSASVPTPLSIFRDLRQ
>Mature_271_residues
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGRQAIGLTGDLPFIGRDRWHAYELSWLDAQGKPCVATATLHVPCDSPSL
IESKSLKLYLNSLNATRFNSAEAVRTRIATDLSTRAGADVAVEFGLPPIDAVGEGESIDALDLSIDDYGPPNAAYLCAHA
QPVVEEVLTSALLKSNCPVTGQPDWASVTLRYRGAPIDREGLLRYLVSFRDHAEFHEQCVERIFNDVLTQCAPQWLVVEA
RYTRRGGLDINPLRSSASVPTPLSIFRDLRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=270, Percent_Identity=52.2222222222222, Blast_Score=250, Evalue=6e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_XANAC (Q8PFX6)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644152.1
- ProteinModelPortal:   Q8PFX6
- SMR:   Q8PFX6
- GeneID:   1157917
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3846
- NMPDR:   fig|190486.1.peg.3796
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- ProtClustDB:   PRK11792
- BioCyc:   XAXO190486:XAC3846-MONOMER
- BRENDA:   1.7.1.13
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 29694; Mature: 29694

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGRQAIGLTGDLPFIGRDRWHAYELSWLDA
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEECCCCCCCCCCCCCCEEEEEEEECC
QGKPCVATATLHVPCDSPSLIESKSLKLYLNSLNATRFNSAEAVRTRIATDLSTRAGADV
CCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCE
AVEFGLPPIDAVGEGESIDALDLSIDDYGPPNAAYLCAHAQPVVEEVLTSALLKSNCPVT
EEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCC
GQPDWASVTLRYRGAPIDREGLLRYLVSFRDHAEFHEQCVERIFNDVLTQCAPQWLVVEA
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
RYTRRGGLDINPLRSSASVPTPLSIFRDLRQ
EECCCCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGRQAIGLTGDLPFIGRDRWHAYELSWLDA
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEECCCCCCCCCCCCCCEEEEEEEECC
QGKPCVATATLHVPCDSPSLIESKSLKLYLNSLNATRFNSAEAVRTRIATDLSTRAGADV
CCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCE
AVEFGLPPIDAVGEGESIDALDLSIDDYGPPNAAYLCAHAQPVVEEVLTSALLKSNCPVT
EEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCC
GQPDWASVTLRYRGAPIDREGLLRYLVSFRDHAEFHEQCVERIFNDVLTQCAPQWLVVEA
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
RYTRRGGLDINPLRSSASVPTPLSIFRDLRQ
EECCCCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217