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The map label for this gene is rpoH [H]

Identifier: 21244548

GI number: 21244548

Start: 4498013

End: 4498888

Strand: Reverse

Name: rpoH [H]

Synonym: XAC3824

Alternate gene names: 21244548

Gene position: 4498888-4498013 (Counterclockwise)

Preceding gene: 21244549

Following gene: 77748744

Centisome position: 86.93

GC content: 63.01

Gene sequence:

>876_bases
ATGAACCAGACTACCTCGACTGCCCTTGTGGCAAACAATCTCCCGATTCCCAGTGCGCTCGGTTCGCTGGACGCCTACAT
CGGTGCCGTGCACCAGATCCCGGTGCTGTCGGTCGATGAGGAGCAGGATCTGGCCCGTCGCTTTCGCGACGAGCTGGATC
TGGACGCCGCGCGCGAACTGGTCCATTCCCACCTGCGCTTCGTGGTGCACGTGGCCCGCGGCTACAACGGCTACGGCCTG
CCGCTGGGCGACCTGATCCAGGAAGGCAACATCGGCCTGATGAAGGCGGTCAAGCGCTTCGACCCGGACATGGGCGTGCG
CCTGGTCAGCTTCGCGGTGCACTGGATCCGTGCCGAGATGCACGAGTTCATCCTGAAGAACTGGCGCATCGTCAAGGTCG
CCACGACCAAGGCGCAGCGCAAACTGTTCTTCAACCTGCGCAAGTCCAAGACGCGTCTGGGCTGGCTCAATGCGTCGGAA
GTGACTGCGGTTGCCAAGGACCTGAACGTCTCCGAGCGTGAGGTGATGGAAATGGAGTCGCGCCTGTCCGGTCGCGACAT
CGGTTTCGACGCGTCGTCGGACGAAGACGACGATCACGGCCCGCCGTCGCCGGTGAGCTACCTGGTCGCCAATGAAGAAG
ATCCGTCGCAGGCGTACGAGCGTCACGACAGCGAAGACAACCAGCTGCAGCTGCTGCGCGAAGGTATGGCTGGCCTGGAC
GCGCGCTCGCGCGACATCGTCAAGCGCCGCTGGCTGGATTCCGATTCCAAGGTGACGCTGCAGGAACTGGCAGACGAATA
CGGCGTGTCGGCCGAGCGCATCCGTCAGATCGAAGCGAATGCGCTGAAGAAGATGAAGGCGCTGTTCGTCGCCTGA

Upstream 100 bases:

>100_bases
AACACCGGCGATGCCAAAAGCCCGAACGTCCTATAAATGGAATGCTGGGAACTCTTTCTGTACCCGGCAGTCCAATAGTT
CGACTGCTAAGATGGTGCCT

Downstream 100 bases:

>100_bases
TCCTGTCGGTCTTGCGCGGGTGTTTGCACAGACGGCCCGGTTTTCCGGGCCGTCTGCCTATGTGGGGCTACGCGATTGCT
ACTCCGGCGAGCGCGCCACC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQDLARRFRDELDLDAARELVHSHLRFVVHVARGYNGYGL
PLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNASE
VTAVAKDLNVSEREVMEMESRLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMAGLD
ARSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA

Sequences:

>Translated_291_residues
MNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQDLARRFRDELDLDAARELVHSHLRFVVHVARGYNGYGL
PLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNASE
VTAVAKDLNVSEREVMEMESRLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMAGLD
ARSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA
>Mature_291_residues
MNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQDLARRFRDELDLDAARELVHSHLRFVVHVARGYNGYGL
PLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNASE
VTAVAKDLNVSEREVMEMESRLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMAGLD
ARSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=270, Percent_Identity=59.6296296296296, Blast_Score=324, Evalue=5e-90,
Organism=Escherichia coli, GI1789098, Length=283, Percent_Identity=27.5618374558304, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789448, Length=257, Percent_Identity=28.4046692607004, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32783; Mature: 32783

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQDLARRFRDELDLDAAREL
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHCCHHHHHHH
VHSHLRFVVHVARGYNGYGLPLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEM
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
HEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNASEVTAVAKDLNVSEREVMEMES
HHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
RLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMAGLD
HCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHCCC
ARSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA
CHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQDLARRFRDELDLDAAREL
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHCCHHHHHHH
VHSHLRFVVHVARGYNGYGLPLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEM
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
HEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNASEVTAVAKDLNVSEREVMEMES
HHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
RLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMAGLD
HCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHCCC
ARSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA
CHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9168128; 10952301 [H]