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The map label for this gene is azoR2

Identifier: 21244498

GI number: 21244498

Start: 4449372

End: 4450022

Strand: Direct

Name: azoR2

Synonym: XAC3773

Alternate gene names: 21244498

Gene position: 4449372-4450022 (Clockwise)

Preceding gene: 77748741

Following gene: 21244499

Centisome position: 85.97

GC content: 65.44

Gene sequence:

>651_bases
ATGCGCTTGCTCCATCTCGATTCCAGCATCCTGACCGACACGTCGGTCTCCCGTCGGCTCACCGCCCAGGTGGTGCAGGA
CCTGCACGCGGCGATTGACGATCTCCATGCCACCTATCGGGACCTTGCCGCGGCTCCCATCGCGCACCTTTCCGGCGCGA
TTGCTGCCGGCTTCCGCCCACTGCCGCAACCCGACAGCGACGCGGCGTCGGTGGCCGAACATGCGTTGTCCGAGCAGCTG
GTGGACGAATTCCTCGCCAGCGACATCGTGGTGATCGGCGCACCGATGTACAACTTTTCGGTGCCGACCCAACTCAAGGC
CTGGATCGATCGCATCGCCCAGCCCGGCCGCACGTTTCGCTATACCGCGAACGGGCCCGAAGGCCTGGCCGGCGGCAAGC
AGCTGATCGTGGCGTCATCGCGTGGGGGCATGTACGCGCAAGGGCCGATGGCTTCACTGGATTTTCAGGAGGCGTACCTG
ACCGCCACATTCGGCTTTCTAGGCGTGCGCGACGTGTACTTTGTGCGCGCGGAAAATCAGTCGCGCGGTCCAGGGCCGGC
TGCGGCAGCATTGATCAGTGCGCAGGCATCCATCGCCGATGTCGTGCGCAGTGTCGCGCGCGGTGTCGTGCACACACGCG
TGGAGGCCTGA

Upstream 100 bases:

>100_bases
TGTCCTGCCAGGCAGGACAGCTTGTCATCCGCCAGCGACCTACCTGCGCACGGGACCACTCCGTACAGTGCCGCCACCGA
CCACAGCACACGAGCCCACC

Downstream 100 bases:

>100_bases
GCCATGTTGCATCTCATCACCCTCACCTATCGGGTCGCCCCGCCGGCATTGGCCGCACATCTGGACGCCCACAAGCAATG
GCTGCGGAAGCATATCGACA

Product: acyl carrier phosphodiesterase-like protein

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 2; FMN-dependent NADH-azo compound oxidoreductase 2

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MRLLHLDSSILTDTSVSRRLTAQVVQDLHAAIDDLHATYRDLAAAPIAHLSGAIAAGFRPLPQPDSDAASVAEHALSEQL
VDEFLASDIVVIGAPMYNFSVPTQLKAWIDRIAQPGRTFRYTANGPEGLAGGKQLIVASSRGGMYAQGPMASLDFQEAYL
TATFGFLGVRDVYFVRAENQSRGPGPAAAALISAQASIADVVRSVARGVVHTRVEA

Sequences:

>Translated_216_residues
MRLLHLDSSILTDTSVSRRLTAQVVQDLHAAIDDLHATYRDLAAAPIAHLSGAIAAGFRPLPQPDSDAASVAEHALSEQL
VDEFLASDIVVIGAPMYNFSVPTQLKAWIDRIAQPGRTFRYTANGPEGLAGGKQLIVASSRGGMYAQGPMASLDFQEAYL
TATFGFLGVRDVYFVRAENQSRGPGPAAAALISAQASIADVVRSVARGVVHTRVEA
>Mature_216_residues
MRLLHLDSSILTDTSVSRRLTAQVVQDLHAAIDDLHATYRDLAAAPIAHLSGAIAAGFRPLPQPDSDAASVAEHALSEQL
VDEFLASDIVVIGAPMYNFSVPTQLKAWIDRIAQPGRTFRYTANGPEGLAGGKQLIVASSRGGMYAQGPMASLDFQEAYL
TATFGFLGVRDVYFVRAENQSRGPGPAAAALISAQASIADVVRSVARGVVHTRVEA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=201, Percent_Identity=43.7810945273632, Blast_Score=147, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR2_XANAC (P58903)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644080.1
- ProteinModelPortal:   P58903
- SMR:   P58903
- GeneID:   1157844
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3773
- NMPDR:   fig|190486.1.peg.3724
- HOGENOM:   HBG565834
- OMA:   EFLASDI
- ProtClustDB:   CLSK813270
- BioCyc:   XAXO190486:XAC3773-MONOMER
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 22898; Mature: 22898

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHLDSSILTDTSVSRRLTAQVVQDLHAAIDDLHATYRDLAAAPIAHLSGAIAAGFRP
CEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPQPDSDAASVAEHALSEQLVDEFLASDIVVIGAPMYNFSVPTQLKAWIDRIAQPGRTFR
CCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEE
YTANGPEGLAGGKQLIVASSRGGMYAQGPMASLDFQEAYLTATFGFLGVRDVYFVRAENQ
EECCCCCCCCCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCC
SRGPGPAAAALISAQASIADVVRSVARGVVHTRVEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLLHLDSSILTDTSVSRRLTAQVVQDLHAAIDDLHATYRDLAAAPIAHLSGAIAAGFRP
CEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPQPDSDAASVAEHALSEQLVDEFLASDIVVIGAPMYNFSVPTQLKAWIDRIAQPGRTFR
CCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEE
YTANGPEGLAGGKQLIVASSRGGMYAQGPMASLDFQEAYLTATFGFLGVRDVYFVRAENQ
EECCCCCCCCCCCEEEEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCC
SRGPGPAAAALISAQASIADVVRSVARGVVHTRVEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217