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The map label for this gene is slt [H]

Identifier: 21244286

GI number: 21244286

Start: 4223637

End: 4225742

Strand: Direct

Name: slt [H]

Synonym: XAC3561

Alternate gene names: 21244286

Gene position: 4223637-4225742 (Clockwise)

Preceding gene: 21244284

Following gene: 21244291

Centisome position: 81.61

GC content: 66.33

Gene sequence:

>2106_bases
GTGACGAGGTGTCATCTGCACGCACGTGCGTGCTCGACCCATTGCGCATGCAAGCGAAGACGGCCATCTCCATCACGCGC
GAAGGACGCGTATTCACTCAGGCGCTCAGGCGTGCTTGGGTATCATCGCGCCATGACTTATGCCCCCCGCCTTGCCACTG
TTTTCATCTGTCTGCTGTGCCTTGGCAGCACCGCATTGCACGCGCAAACCCTCGACCCACAGCTGACTGCCATGCGCGAT
GCCATCGCGGCCGCAGAGCGCGGCCAGACCGATGCCGGCCAGCTCGCTGCATTGAGCCGCCATCCGCTGTATGGATGGCT
GGAATTTGCCGCGCTCAAGCGCAGTATCGACACCGTCTCCAATGCGCAGGCGCAAGCATTTTTGCAGCGTTATGCCGGCC
AGCCCGTGGCTGAGAGCTTCCGCAACACGTGGCTGCCGGCGGTGGCGCGGCGGCAAGACTGGAACACGCTGCTGGCCAAC
TGGAAGTCCACCGACAACGCCGGCCTGCGCTGCGCGCAACTGACCGCGCGCCTGGCCACCGGCAAGGCCGATGCACAGTG
GAGCCGCGATGCGCTGACGCTGTGGCGCAACGGCAAGGCGCTGCCCGATGCCTGCGATGCAGTGGTTGCCGGGCTGGAAT
CGCGCGGTGAACTTACGCCGGCGCTGCGCTGGGAACGTGTGGAAGCCGCTGCCGATGCACAGCAGCCTGCGGTGATGCGC
ACCGCCGCGCGTGGTCTGCCGGCGGCCGATCTGACGCTGGCCATCGATTACGCTGCCTTTCTCGACAAGATCCACCCGCG
CGCACTAGGTTGGCCCAAGACCGAGCGCAGCCGCCGCATCGCGGTCGACGGGCTGGCCAAGTTGGCCAAGAGCGATCCCG
ACGCTGCCGAGCAGCAGCTACCGCAACTTGCCAGTGCGCTCGGCTTCAGCCAGGCGCAGCGTGGGCAGGTGCTGTATCAG
ATCGCATTGTGGACCGTCGCCTCGTATCTGCCGGATTCGGCGCATCGGCTCAATGCCGTACCGGACGCGTCCTACGACGA
ACGCCTGCACGAATGGCGTGCACGTGAGGCGATGTCGCGCGGCGACTGGCCGGCGGCGTTGGCCGCGATTCGCAAGATGC
CGACGAGCCAGCGCAGCGACTCGCGCTGGCAGTACTTCGAAGCCCGCCTGGCCGAAAAGACCGGCGCTGCGGCGGCGGCC
CAGCCGCTGTATCGCGCCGCCGCGCAATCGCCGACCTTCCACGGCTTCCTGGCCGCAGACCGCCTGCAGCAACCTTACCG
GCTTTGCCCCTGGGAGCCCAACGACAGCGCGCAGGCAAAGGCCGCCGTTGCGCGCGACCCGGCCCTGGTCCGCGCAATCG
CGTTATTTCAGATCGATCGCCCCGGTTGGGCCGTGGCCGAGTGGAACGATGCCGCCACCCGCTTCGACGACACCCAGCGC
CGGCTGGCAGTGGAAGTTGCCAGCGACAACGGCTGGTTCGACCGTGCGGTGTTCGCGCTCGGCAAGCAGCCGGACGAACA
ACGGCTCTATGCGTTGCGTTTCCCCCTGCACCACGACGACAGCATTCGTCGCGAGGCCGCCAAGAATGCGATCGACCCAG
CCTGGGTGGCTGCGGAAATCCGTGCCGAAAGCATCTTCAATCCACGCGCGCGCTCGCCGGCCAACGCAATGGGCCTGATG
CAGGTATTGCCGGGCACTGGCGCGGCCGTCGCCAAGGGGATCGCCCTGCCCGGCTATGCGGGTGCAAGCAGCCTCTATGA
GCCGGACACCAACATCGCCATCGGCACGGCGTATCTGCGCCAGCTGCTCAACACCTACGGGCTGCCCTACCTCACCATTG
CCGCCTACAATGCGGGGCCCGGCCCCACCGGACGCTGGCAGTCGCAGCGGCCGAATTTCGAGCCGGACTTCTGGATCGAA
ACCATCAGCTACAAGGAAACCCGCGACTATGTCGCGCGCGTGTTGGCTTTCAGCGTGATCTACGACTGGCGCCTCAACGG
CGACATGCTGCCGTTGAGCGACCGGCTCATGGGCAAGCTGGTGGACAAACGTCTGAAACCGGTATGTACCCCGGGCCAGG
CTGCCAATAACGCCGCGCAGGATTGA

Upstream 100 bases:

>100_bases
TGTCCGCACGCCCCCGTGCCCTGCGGTCCTTCCCAATTCTTAAACGAAAAATTAACGCGCCGCAACTGGCGGTGCAGCAA
CGCACGGTGCCGCAGCGATC

Downstream 100 bases:

>100_bases
GCGACGCCGGGCGCCCTGCACGGTTGCGGGGCCCTGGCGCGTGCGCGGCACCGCCATTGCGCACGGCACTGACGCAACAT
GGACGCCACGCAGGGAGCGG

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MTRCHLHARACSTHCACKRRRPSPSRAKDAYSLRRSGVLGYHRAMTYAPRLATVFICLLCLGSTALHAQTLDPQLTAMRD
AIAAAERGQTDAGQLAALSRHPLYGWLEFAALKRSIDTVSNAQAQAFLQRYAGQPVAESFRNTWLPAVARRQDWNTLLAN
WKSTDNAGLRCAQLTARLATGKADAQWSRDALTLWRNGKALPDACDAVVAGLESRGELTPALRWERVEAAADAQQPAVMR
TAARGLPAADLTLAIDYAAFLDKIHPRALGWPKTERSRRIAVDGLAKLAKSDPDAAEQQLPQLASALGFSQAQRGQVLYQ
IALWTVASYLPDSAHRLNAVPDASYDERLHEWRAREAMSRGDWPAALAAIRKMPTSQRSDSRWQYFEARLAEKTGAAAAA
QPLYRAAAQSPTFHGFLAADRLQQPYRLCPWEPNDSAQAKAAVARDPALVRAIALFQIDRPGWAVAEWNDAATRFDDTQR
RLAVEVASDNGWFDRAVFALGKQPDEQRLYALRFPLHHDDSIRREAAKNAIDPAWVAAEIRAESIFNPRARSPANAMGLM
QVLPGTGAAVAKGIALPGYAGASSLYEPDTNIAIGTAYLRQLLNTYGLPYLTIAAYNAGPGPTGRWQSQRPNFEPDFWIE
TISYKETRDYVARVLAFSVIYDWRLNGDMLPLSDRLMGKLVDKRLKPVCTPGQAANNAAQD

Sequences:

>Translated_701_residues
MTRCHLHARACSTHCACKRRRPSPSRAKDAYSLRRSGVLGYHRAMTYAPRLATVFICLLCLGSTALHAQTLDPQLTAMRD
AIAAAERGQTDAGQLAALSRHPLYGWLEFAALKRSIDTVSNAQAQAFLQRYAGQPVAESFRNTWLPAVARRQDWNTLLAN
WKSTDNAGLRCAQLTARLATGKADAQWSRDALTLWRNGKALPDACDAVVAGLESRGELTPALRWERVEAAADAQQPAVMR
TAARGLPAADLTLAIDYAAFLDKIHPRALGWPKTERSRRIAVDGLAKLAKSDPDAAEQQLPQLASALGFSQAQRGQVLYQ
IALWTVASYLPDSAHRLNAVPDASYDERLHEWRAREAMSRGDWPAALAAIRKMPTSQRSDSRWQYFEARLAEKTGAAAAA
QPLYRAAAQSPTFHGFLAADRLQQPYRLCPWEPNDSAQAKAAVARDPALVRAIALFQIDRPGWAVAEWNDAATRFDDTQR
RLAVEVASDNGWFDRAVFALGKQPDEQRLYALRFPLHHDDSIRREAAKNAIDPAWVAAEIRAESIFNPRARSPANAMGLM
QVLPGTGAAVAKGIALPGYAGASSLYEPDTNIAIGTAYLRQLLNTYGLPYLTIAAYNAGPGPTGRWQSQRPNFEPDFWIE
TISYKETRDYVARVLAFSVIYDWRLNGDMLPLSDRLMGKLVDKRLKPVCTPGQAANNAAQD
>Mature_700_residues
TRCHLHARACSTHCACKRRRPSPSRAKDAYSLRRSGVLGYHRAMTYAPRLATVFICLLCLGSTALHAQTLDPQLTAMRDA
IAAAERGQTDAGQLAALSRHPLYGWLEFAALKRSIDTVSNAQAQAFLQRYAGQPVAESFRNTWLPAVARRQDWNTLLANW
KSTDNAGLRCAQLTARLATGKADAQWSRDALTLWRNGKALPDACDAVVAGLESRGELTPALRWERVEAAADAQQPAVMRT
AARGLPAADLTLAIDYAAFLDKIHPRALGWPKTERSRRIAVDGLAKLAKSDPDAAEQQLPQLASALGFSQAQRGQVLYQI
ALWTVASYLPDSAHRLNAVPDASYDERLHEWRAREAMSRGDWPAALAAIRKMPTSQRSDSRWQYFEARLAEKTGAAAAAQ
PLYRAAAQSPTFHGFLAADRLQQPYRLCPWEPNDSAQAKAAVARDPALVRAIALFQIDRPGWAVAEWNDAATRFDDTQRR
LAVEVASDNGWFDRAVFALGKQPDEQRLYALRFPLHHDDSIRREAAKNAIDPAWVAAEIRAESIFNPRARSPANAMGLMQ
VLPGTGAAVAKGIALPGYAGASSLYEPDTNIAIGTAYLRQLLNTYGLPYLTIAAYNAGPGPTGRWQSQRPNFEPDFWIET
ISYKETRDYVARVLAFSVIYDWRLNGDMLPLSDRLMGKLVDKRLKPVCTPGQAANNAAQD

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=635, Percent_Identity=28.3464566929134, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI87082191, Length=113, Percent_Identity=38.0530973451327, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 77218; Mature: 77087

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRCHLHARACSTHCACKRRRPSPSRAKDAYSLRRSGVLGYHRAMTYAPRLATVFICLLC
CCCEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LGSTALHAQTLDPQLTAMRDAIAAAERGQTDAGQLAALSRHPLYGWLEFAALKRSIDTVS
HCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
NAQAQAFLQRYAGQPVAESFRNTWLPAVARRQDWNTLLANWKSTDNAGLRCAQLTARLAT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHC
GKADAQWSRDALTLWRNGKALPDACDAVVAGLESRGELTPALRWERVEAAADAQQPAVMR
CCCCCHHCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHH
TAARGLPAADLTLAIDYAAFLDKIHPRALGWPKTERSRRIAVDGLAKLAKSDPDAAEQQL
HHHCCCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCEEHHHHHHHHHCCCCCHHHHHH
PQLASALGFSQAQRGQVLYQIALWTVASYLPDSAHRLNAVPDASYDERLHEWRAREAMSR
HHHHHHHCHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHC
GDWPAALAAIRKMPTSQRSDSRWQYFEARLAEKTGAAAAAQPLYRAAAQSPTFHGFLAAD
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHH
RLQQPYRLCPWEPNDSAQAKAAVARDPALVRAIALFQIDRPGWAVAEWNDAATRFDDTQR
HHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHEECCCCCEEEECCCHHHHHHHHHH
RLAVEVASDNGWFDRAVFALGKQPDEQRLYALRFPLHHDDSIRREAAKNAIDPAWVAAEI
EEEEEEECCCCCHHHHHHHHCCCCCHHCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHH
RAESIFNPRARSPANAMGLMQVLPGTGAAVAKGIALPGYAGASSLYEPDTNIAIGTAYLR
HHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHH
QLLNTYGLPYLTIAAYNAGPGPTGRWQSQRPNFEPDFWIETISYKETRDYVARVLAFSVI
HHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHH
YDWRLNGDMLPLSDRLMGKLVDKRLKPVCTPGQAANNAAQD
HEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRCHLHARACSTHCACKRRRPSPSRAKDAYSLRRSGVLGYHRAMTYAPRLATVFICLLC
CCEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LGSTALHAQTLDPQLTAMRDAIAAAERGQTDAGQLAALSRHPLYGWLEFAALKRSIDTVS
HCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
NAQAQAFLQRYAGQPVAESFRNTWLPAVARRQDWNTLLANWKSTDNAGLRCAQLTARLAT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHC
GKADAQWSRDALTLWRNGKALPDACDAVVAGLESRGELTPALRWERVEAAADAQQPAVMR
CCCCCHHCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHH
TAARGLPAADLTLAIDYAAFLDKIHPRALGWPKTERSRRIAVDGLAKLAKSDPDAAEQQL
HHHCCCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCEEHHHHHHHHHCCCCCHHHHHH
PQLASALGFSQAQRGQVLYQIALWTVASYLPDSAHRLNAVPDASYDERLHEWRAREAMSR
HHHHHHHCHHHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHC
GDWPAALAAIRKMPTSQRSDSRWQYFEARLAEKTGAAAAAQPLYRAAAQSPTFHGFLAAD
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHH
RLQQPYRLCPWEPNDSAQAKAAVARDPALVRAIALFQIDRPGWAVAEWNDAATRFDDTQR
HHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHEECCCCCEEEECCCHHHHHHHHHH
RLAVEVASDNGWFDRAVFALGKQPDEQRLYALRFPLHHDDSIRREAAKNAIDPAWVAAEI
EEEEEEECCCCCHHHHHHHHCCCCCHHCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHH
RAESIFNPRARSPANAMGLMQVLPGTGAAVAKGIALPGYAGASSLYEPDTNIAIGTAYLR
HHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHH
QLLNTYGLPYLTIAAYNAGPGPTGRWQSQRPNFEPDFWIETISYKETRDYVARVLAFSVI
HHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHH
YDWRLNGDMLPLSDRLMGKLVDKRLKPVCTPGQAANNAAQD
HEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]