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The map label for this gene is hemL

Identifier: 21244145

GI number: 21244145

Start: 4024628

End: 4025917

Strand: Direct

Name: hemL

Synonym: XAC3420

Alternate gene names: 21244145

Gene position: 4024628-4025917 (Clockwise)

Preceding gene: 21244142

Following gene: 21244146

Centisome position: 77.76

GC content: 67.36

Gene sequence:

>1290_bases
ATGAACCATTCCCGCTCCCACGCCCTGTTCGCCCAGGCGCAGACCTTGCTACCCGGCGGCGTCAACTCGCCGGTACGGGC
GTTCAAGTCGGTCGGCGGCGAGCCATTCTTCGTGGCGCGCGCCGATGGCCCATATCTGTTCGATGTCGACGACAACCGCT
ACATCGACTACGTGGGCTCGTGGGGGCCGATGATTGCCGGGCACAACCACCCCGCCGTGCGCGAAGCGGTCGAGCAGTCG
ATCCGCAACGGGCTCTCGTTCGGTGCACCGTGCGCAGCCGAAGTAGCGATGGCGCAGACCATCACCCGGCTGGTGCCCTC
CTGCGAGATGGTGCGCATGGTCAACTCCGGCACCGAAGCGACGCTGTCGGCGGTACGCCTGGCGCGTGGCGCCACCGGGC
GCAATCGCATCATCAAGTTCGAAGGCTGTTATCACGGCCATGGCGACTCGTTTCTGGTCAAGGCCGGCAGCGGCATGCTG
ACCCTGGGCGTACCGACCTCGCCCGGCGTACCCGCAGGGCTGAGCGAGCTGACCGCCACGCTGAGCTTCAACGACTTCGA
GGGCGCCACCGCGCTGTTCGACGAGATTGGTGCGGAGGTGGCGGCGGTGATCATCGAGCCGGTGGTCGGCAACGCCAACT
GCATTCCGCCGCAGGCCGGCTATCTGCAGCATCTGCGCACGCTGTGCACGCGGCACGGCGCGTTGCTGATCTTCGACGAA
GTGATGACCGGCTTCCGCGTCGCGCTCGGTGGTGCGCAGGCGCATTACGGCGTCACCCCGGACCTGACCACCTTCGGCAA
GATCATCGGCGGCGGCATGCCGGTGGGCGCCTACGGCGGCCGCCGCGACTTGATGGAGCAGGTTGCTCCGGCCGGGCCGA
TCTACCAGGCCGGCACCTTGTCGGGCAACCCGGTGGCCATGGCTGCAGGCCTGGCGATGCTGGAGCTGGTGCAGGAGCCG
GGCTTCCACACGCGCTTGAGCGAGGCCACCAGCATGCTGTGCGAAGGCCTGGAAGATGCTGCGCGCGCAGCCGGCATCGC
CGTCACCACCAACCAGGTTGGCGGCATGTTCGGGCTGTTCTTCACCGACGACATCGTGGAGAGCTACGCACAGGCCACGG
CGTGCGACATCACCACCTTCAACCGATTCTTTCACGCGATGCTGCAGCAGGGCGTGTACCTGGCGCCCTCGGCGTACGAA
GCCGGCTTCATGTCCAGTGCGCACGACGAGGCGGTGATCGACGCCACGCTGGCGGCAGCGCGCGAGGCATTTGCGGAAGT
GGCGCGCTGA

Upstream 100 bases:

>100_bases
CTGCGGGTAGGCGCGCGAGGCCGCAGGCCTGACAATGGCGGACGCGATGCCCGCTGCCGGGCGTCGATGCCCCCAATCGG
TACTCCTCAAGGATCTGCGC

Downstream 100 bases:

>100_bases
TCGGCTGGGAATCGGGAATGGGGAGTTGGGAATCGGTGAGCAGTTAGCGCGCGGCCTTGAGGGTTTGCGGTGCGCGTGAT
CGTGCCGCACTTGCGGTTGC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNHSRSHALFAQAQTLLPGGVNSPVRAFKSVGGEPFFVARADGPYLFDVDDNRYIDYVGSWGPMIAGHNHPAVREAVEQS
IRNGLSFGAPCAAEVAMAQTITRLVPSCEMVRMVNSGTEATLSAVRLARGATGRNRIIKFEGCYHGHGDSFLVKAGSGML
TLGVPTSPGVPAGLSELTATLSFNDFEGATALFDEIGAEVAAVIIEPVVGNANCIPPQAGYLQHLRTLCTRHGALLIFDE
VMTGFRVALGGAQAHYGVTPDLTTFGKIIGGGMPVGAYGGRRDLMEQVAPAGPIYQAGTLSGNPVAMAAGLAMLELVQEP
GFHTRLSEATSMLCEGLEDAARAAGIAVTTNQVGGMFGLFFTDDIVESYAQATACDITTFNRFFHAMLQQGVYLAPSAYE
AGFMSSAHDEAVIDATLAAAREAFAEVAR

Sequences:

>Translated_429_residues
MNHSRSHALFAQAQTLLPGGVNSPVRAFKSVGGEPFFVARADGPYLFDVDDNRYIDYVGSWGPMIAGHNHPAVREAVEQS
IRNGLSFGAPCAAEVAMAQTITRLVPSCEMVRMVNSGTEATLSAVRLARGATGRNRIIKFEGCYHGHGDSFLVKAGSGML
TLGVPTSPGVPAGLSELTATLSFNDFEGATALFDEIGAEVAAVIIEPVVGNANCIPPQAGYLQHLRTLCTRHGALLIFDE
VMTGFRVALGGAQAHYGVTPDLTTFGKIIGGGMPVGAYGGRRDLMEQVAPAGPIYQAGTLSGNPVAMAAGLAMLELVQEP
GFHTRLSEATSMLCEGLEDAARAAGIAVTTNQVGGMFGLFFTDDIVESYAQATACDITTFNRFFHAMLQQGVYLAPSAYE
AGFMSSAHDEAVIDATLAAAREAFAEVAR
>Mature_429_residues
MNHSRSHALFAQAQTLLPGGVNSPVRAFKSVGGEPFFVARADGPYLFDVDDNRYIDYVGSWGPMIAGHNHPAVREAVEQS
IRNGLSFGAPCAAEVAMAQTITRLVPSCEMVRMVNSGTEATLSAVRLARGATGRNRIIKFEGCYHGHGDSFLVKAGSGML
TLGVPTSPGVPAGLSELTATLSFNDFEGATALFDEIGAEVAAVIIEPVVGNANCIPPQAGYLQHLRTLCTRHGALLIFDE
VMTGFRVALGGAQAHYGVTPDLTTFGKIIGGGMPVGAYGGRRDLMEQVAPAGPIYQAGTLSGNPVAMAAGLAMLELVQEP
GFHTRLSEATSMLCEGLEDAARAAGIAVTTNQVGGMFGLFFTDDIVESYAQATACDITTFNRFFHAMLQQGVYLAPSAYE
AGFMSSAHDEAVIDATLAAAREAFAEVAR

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=297, Percent_Identity=29.2929292929293, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI37574042, Length=367, Percent_Identity=26.4305177111717, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI226442705, Length=366, Percent_Identity=25.6830601092896, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI226442709, Length=334, Percent_Identity=26.6467065868263, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI284507298, Length=215, Percent_Identity=31.1627906976744, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=62.8235294117647, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI1789016, Length=345, Percent_Identity=32.1739130434783, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788044, Length=339, Percent_Identity=32.4483775811209, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1787560, Length=345, Percent_Identity=31.5942028985507, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI145693181, Length=333, Percent_Identity=30.3303303303303, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789759, Length=297, Percent_Identity=29.2929292929293, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786991, Length=383, Percent_Identity=28.4595300261097, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71992977, Length=364, Percent_Identity=27.7472527472527, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=350, Percent_Identity=26, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI25144274, Length=199, Percent_Identity=28.643216080402, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=343, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=363, Percent_Identity=23.9669421487603, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21357415, Length=293, Percent_Identity=26.962457337884, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI161085790, Length=365, Percent_Identity=25.7534246575342, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574759, Length=365, Percent_Identity=25.7534246575342, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_XANAC (Q8PH40)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643727.1
- ProteinModelPortal:   Q8PH40
- SMR:   Q8PH40
- GeneID:   1157491
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3420
- NMPDR:   fig|190486.1.peg.3371
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- BioCyc:   XAXO190486:XAC3420-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45045; Mature: 45045

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSRSHALFAQAQTLLPGGVNSPVRAFKSVGGEPFFVARADGPYLFDVDDNRYIDYVGS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCCEEEEECC
WGPMIAGHNHPAVREAVEQSIRNGLSFGAPCAAEVAMAQTITRLVPSCEMVRMVNSGTEA
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCHH
TLSAVRLARGATGRNRIIKFEGCYHGHGDSFLVKAGSGMLTLGVPTSPGVPAGLSELTAT
HHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCCCCHHHHEEE
LSFNDFEGATALFDEIGAEVAAVIIEPVVGNANCIPPQAGYLQHLRTLCTRHGALLIFDE
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHH
VMTGFRVALGGAQAHYGVTPDLTTFGKIIGGGMPVGAYGGRRDLMEQVAPAGPIYQAGTL
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCC
SGNPVAMAAGLAMLELVQEPGFHTRLSEATSMLCEGLEDAARAAGIAVTTNQVGGMFGLF
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEHHH
FTDDIVESYAQATACDITTFNRFFHAMLQQGVYLAPSAYEAGFMSSAHDEAVIDATLAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHCCCCCCCCHHHHHHHHHHH
REAFAEVAR
HHHHHHHCC
>Mature Secondary Structure
MNHSRSHALFAQAQTLLPGGVNSPVRAFKSVGGEPFFVARADGPYLFDVDDNRYIDYVGS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCCEEEEECC
WGPMIAGHNHPAVREAVEQSIRNGLSFGAPCAAEVAMAQTITRLVPSCEMVRMVNSGTEA
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCHH
TLSAVRLARGATGRNRIIKFEGCYHGHGDSFLVKAGSGMLTLGVPTSPGVPAGLSELTAT
HHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCCCCHHHHEEE
LSFNDFEGATALFDEIGAEVAAVIIEPVVGNANCIPPQAGYLQHLRTLCTRHGALLIFDE
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHH
VMTGFRVALGGAQAHYGVTPDLTTFGKIIGGGMPVGAYGGRRDLMEQVAPAGPIYQAGTL
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCC
SGNPVAMAAGLAMLELVQEPGFHTRLSEATSMLCEGLEDAARAAGIAVTTNQVGGMFGLF
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEHHH
FTDDIVESYAQATACDITTFNRFFHAMLQQGVYLAPSAYEAGFMSSAHDEAVIDATLAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHCCCCCCCCHHHHHHHHHHH
REAFAEVAR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217