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The map label for this gene is salR [H]

Identifier: 21244035

GI number: 21244035

Start: 3888389

End: 3889441

Strand: Direct

Name: salR [H]

Synonym: XAC3310

Alternate gene names: 21244035

Gene position: 3888389-3889441 (Clockwise)

Preceding gene: 21244034

Following gene: 21244038

Centisome position: 75.13

GC content: 66.29

Gene sequence:

>1053_bases
ATGGTGGCCAAGCGGCAACGCAGTGCGAACAATGCGGCGACGTTGTTGGATGTCGCCAAACATGCCGGCGTCTCGCCGAT
GACCGCTTCGCGTGTGATCAACCGTCACCCGCATGTGGGCGAAGCGATGCGCGCGCGCGTGGAGGCCTCGGTACAGGCAT
TGGGCTACCGGCCCAATCTCGCCGGCCGTTCGTTGCGGACCAGCGGGCTGCTGCGTATCGGCGTGCTGTACAGCAACCCG
AGCGCCGCGTACCTCAATCAATTCATGCTCGGCCTGCTCGAGCAGAGCAGCCTCACCGGCAGCCAGGTCATGGTGGAGAA
GTGCGGCGCCATCCGCAGCCAGCGAGCAGCCACCGAGCGCCTGCTCGCTGCCGGCGTCGACGGGGTGATCCTGCCACCGC
CGTTATGCGACTCGCGTCAGACCATCACCGAACTCGATGCGCGCGGCATTCCGGTCGTGGCGGTGGCCAGTGGCGCACCG
ATGGCGCAAATCAGCTCGGTGCGGATCGACGACTACCAGGCTGCGCGCGCCATCGTCGACCACCTGATCGAGCTCGGGCA
TCGCCGTATTGCGTTGATCAAGGGCGACCCGAAACACACCCCGAGCGTACTGCGCGCAAATGGCTATCTCGACAGCCTGC
GCGCTGCCGGCTTATCGGTCGCAGATGGCTTGATCGCCGAGGGCTTGTTCACCTATCACTCCGGGCTCCTCGCCGCGCGC
AGCCTGTTGACGCAGCCGCAGCCAGCCACTGCGATCTTCTGCAGCAACGACGACATGGCGGCCGCAGCGGTCGCGGTCGC
GCATGGATTGGGCTTGCGCGTGCCCGAAGATGTCTCCATCGCCGGCTTCGACGATACGCCGGTGGCCACCACCATCTGGC
CCGAGCTCACCACCGTGCATCAACCGGTCACCGCCATGGGGCGCGCTGCTGTGTCACTGCTGGCCGACGCGGTGCGTCAA
CGCCGCAAGGGCGACACCGCACCCGGCGTGCATCAGGTGATGAAATACACGCTGGTCAAGCGTGGCTCGACGGCAGGGCC
GTCGAAGACATAG

Upstream 100 bases:

>100_bases
CTGGTACTGCGTGATGTGATTGGTCGGTTGCTTTTCGTTGTCGCTTCACACCTCGAACCTATAGCGCGCATTCGCTCTTA
CCCCAGGATGAGCACACACA

Downstream 100 bases:

>100_bases
CGCGACAGCGCACCATCGCCATCCCGGTTCGGCACAGGCGCACCATCTCCATCTGCGCGCACGCTGCGCCGTCGCAGCCA
ACCGCCGCCCTTTCAACAGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MVAKRQRSANNAATLLDVAKHAGVSPMTASRVINRHPHVGEAMRARVEASVQALGYRPNLAGRSLRTSGLLRIGVLYSNP
SAAYLNQFMLGLLEQSSLTGSQVMVEKCGAIRSQRAATERLLAAGVDGVILPPPLCDSRQTITELDARGIPVVAVASGAP
MAQISSVRIDDYQAARAIVDHLIELGHRRIALIKGDPKHTPSVLRANGYLDSLRAAGLSVADGLIAEGLFTYHSGLLAAR
SLLTQPQPATAIFCSNDDMAAAAVAVAHGLGLRVPEDVSIAGFDDTPVATTIWPELTTVHQPVTAMGRAAVSLLADAVRQ
RRKGDTAPGVHQVMKYTLVKRGSTAGPSKT

Sequences:

>Translated_350_residues
MVAKRQRSANNAATLLDVAKHAGVSPMTASRVINRHPHVGEAMRARVEASVQALGYRPNLAGRSLRTSGLLRIGVLYSNP
SAAYLNQFMLGLLEQSSLTGSQVMVEKCGAIRSQRAATERLLAAGVDGVILPPPLCDSRQTITELDARGIPVVAVASGAP
MAQISSVRIDDYQAARAIVDHLIELGHRRIALIKGDPKHTPSVLRANGYLDSLRAAGLSVADGLIAEGLFTYHSGLLAAR
SLLTQPQPATAIFCSNDDMAAAAVAVAHGLGLRVPEDVSIAGFDDTPVATTIWPELTTVHQPVTAMGRAAVSLLADAVRQ
RRKGDTAPGVHQVMKYTLVKRGSTAGPSKT
>Mature_350_residues
MVAKRQRSANNAATLLDVAKHAGVSPMTASRVINRHPHVGEAMRARVEASVQALGYRPNLAGRSLRTSGLLRIGVLYSNP
SAAYLNQFMLGLLEQSSLTGSQVMVEKCGAIRSQRAATERLLAAGVDGVILPPPLCDSRQTITELDARGIPVVAVASGAP
MAQISSVRIDDYQAARAIVDHLIELGHRRIALIKGDPKHTPSVLRANGYLDSLRAAGLSVADGLIAEGLFTYHSGLLAAR
SLLTQPQPATAIFCSNDDMAAAAVAVAHGLGLRVPEDVSIAGFDDTPVATTIWPELTTVHQPVTAMGRAAVSLLADAVRQ
RRKGDTAPGVHQVMKYTLVKRGSTAGPSKT

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=347, Percent_Identity=34.0057636887608, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787948, Length=349, Percent_Identity=32.6647564469914, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1790194, Length=313, Percent_Identity=33.5463258785943, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1789068, Length=339, Percent_Identity=30.3834808259587, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1786540, Length=341, Percent_Identity=32.5513196480938, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789202, Length=338, Percent_Identity=28.9940828402367, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI48994940, Length=331, Percent_Identity=30.2114803625378, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1787580, Length=308, Percent_Identity=25.6493506493506, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788474, Length=338, Percent_Identity=28.698224852071, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790715, Length=312, Percent_Identity=26.9230769230769, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787906, Length=285, Percent_Identity=28.4210526315789, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789456, Length=320, Percent_Identity=26.25, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36875; Mature: 36875

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAKRQRSANNAATLLDVAKHAGVSPMTASRVINRHPHVGEAMRARVEASVQALGYRPNL
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
AGRSLRTSGLLRIGVLYSNPSAAYLNQFMLGLLEQSSLTGSQVMVEKCGAIRSQRAATER
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LLAAGVDGVILPPPLCDSRQTITELDARGIPVVAVASGAPMAQISSVRIDDYQAARAIVD
HHHHCCCCCEECCCCCCCCHHHHHHCCCCCCEEEEECCCCHHHHHCEECCHHHHHHHHHH
HLIELGHRRIALIKGDPKHTPSVLRANGYLDSLRAAGLSVADGLIAEGLFTYHSGLLAAR
HHHHCCCCEEEEEECCCCCCCHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SLLTQPQPATAIFCSNDDMAAAAVAVAHGLGLRVPEDVSIAGFDDTPVATTIWPELTTVH
HHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEECCHHHHHH
QPVTAMGRAAVSLLADAVRQRRKGDTAPGVHQVMKYTLVKRGSTAGPSKT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVAKRQRSANNAATLLDVAKHAGVSPMTASRVINRHPHVGEAMRARVEASVQALGYRPNL
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
AGRSLRTSGLLRIGVLYSNPSAAYLNQFMLGLLEQSSLTGSQVMVEKCGAIRSQRAATER
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LLAAGVDGVILPPPLCDSRQTITELDARGIPVVAVASGAPMAQISSVRIDDYQAARAIVD
HHHHCCCCCEECCCCCCCCHHHHHHCCCCCCEEEEECCCCHHHHHCEECCHHHHHHHHHH
HLIELGHRRIALIKGDPKHTPSVLRANGYLDSLRAAGLSVADGLIAEGLFTYHSGLLAAR
HHHHCCCCEEEEEECCCCCCCHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SLLTQPQPATAIFCSNDDMAAAAVAVAHGLGLRVPEDVSIAGFDDTPVATTIWPELTTVH
HHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEECCHHHHHH
QPVTAMGRAAVSLLADAVRQRRKGDTAPGVHQVMKYTLVKRGSTAGPSKT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA