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The map label for this gene is mscL

Identifier: 21244033

GI number: 21244033

Start: 3886063

End: 3886494

Strand: Direct

Name: mscL

Synonym: XAC3308

Alternate gene names: 21244033

Gene position: 3886063-3886494 (Clockwise)

Preceding gene: 21244032

Following gene: 21244034

Centisome position: 75.08

GC content: 59.95

Gene sequence:

>432_bases
ATGGGAATGGTCAGCGAATTCAAGCAATTCGCAATGCGCGGCAATGTCATCGACCTGGCGGTCGGTGTGGTCATTGGTGC
GGCGTTCGGCAAGATCGTCACCGCGCTGGTGGAGAAAATCATCATGCCGCCGATCGGTTGGGCCATCGGCAATGTGGATT
TTTCGCGCCTGGCGTGGGTGCTCAAGCCGGCCGGCGTCGATGCCACCGGCAAGGAGATCCCAGCCGTTGCCATTGGCTAC
GGCGATTTCATCAACACCGTCGTGCAGTTCCTGATCATCGCCTTCGCAATCTTTCTGGTGGTCAAGTTGATCAACCGCGT
GACCCATCGCAAGCCGGACGCGCCGAAAGGCCCGAGCGAAGAAGTGCTGTTGCTGCGCGAGATTCGCGACGCGCTCAAGA
ACGACACGCTCAAGCCGCCGGGCGCGCTATAA

Upstream 100 bases:

>100_bases
GGAGAACGTCGCCGAGCGCCACGGCATCATCGCGGGCTGACATCGCACCGGCTACGATCGGGCTGCGGCAGTTGGTCCGC
GACCGCAGTTGGAGTGCACT

Downstream 100 bases:

>100_bases
GCGGGCACAACCAACTGGCGCATCCTGTGGCGCTTCTTCGCGCAGAACAATGACCTTTCGCACGCAGACGAGGGACCTCG
CGCTGATAAGCTCGGGGTCC

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MGMVSEFKQFAMRGNVIDLAVGVVIGAAFGKIVTALVEKIIMPPIGWAIGNVDFSRLAWVLKPAGVDATGKEIPAVAIGY
GDFINTVVQFLIIAFAIFLVVKLINRVTHRKPDAPKGPSEEVLLLREIRDALKNDTLKPPGAL

Sequences:

>Translated_143_residues
MGMVSEFKQFAMRGNVIDLAVGVVIGAAFGKIVTALVEKIIMPPIGWAIGNVDFSRLAWVLKPAGVDATGKEIPAVAIGY
GDFINTVVQFLIIAFAIFLVVKLINRVTHRKPDAPKGPSEEVLLLREIRDALKNDTLKPPGAL
>Mature_142_residues
GMVSEFKQFAMRGNVIDLAVGVVIGAAFGKIVTALVEKIIMPPIGWAIGNVDFSRLAWVLKPAGVDATGKEIPAVAIGYG
DFINTVVQFLIIAFAIFLVVKLINRVTHRKPDAPKGPSEEVLLLREIRDALKNDTLKPPGAL

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=138, Percent_Identity=55.0724637681159, Blast_Score=144, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_XANAC (Q8PHE9)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643615.1
- ProteinModelPortal:   Q8PHE9
- SMR:   Q8PHE9
- GeneID:   1157379
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3308
- HOGENOM:   HBG727604
- OMA:   IQLLTEI
- ProtClustDB:   PRK00567
- BioCyc:   XAXO190486:XAC3308-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15393; Mature: 15261

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11308a08)-; HASH(0x124a1d7c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMVSEFKQFAMRGNVIDLAVGVVIGAAFGKIVTALVEKIIMPPIGWAIGNVDFSRLAWV
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHH
LKPAGVDATGKEIPAVAIGYGDFINTVVQFLIIAFAIFLVVKLINRVTHRKPDAPKGPSE
HCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
EVLLLREIRDALKNDTLKPPGAL
HHHHHHHHHHHHHCCCCCCCCCH
>Mature Secondary Structure 
GMVSEFKQFAMRGNVIDLAVGVVIGAAFGKIVTALVEKIIMPPIGWAIGNVDFSRLAWV
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHH
LKPAGVDATGKEIPAVAIGYGDFINTVVQFLIIAFAIFLVVKLINRVTHRKPDAPKGPSE
HCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
EVLLLREIRDALKNDTLKPPGAL
HHHHHHHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12024217