Click here to switch to the map view.

The map label for this gene is metB [H]

Identifier: 21243766

GI number: 21243766

Start: 3555585

End: 3556802

Strand: Reverse

Name: metB [H]

Synonym: XAC3039

Alternate gene names: 21243766

Gene position: 3556802-3555585 (Counterclockwise)

Preceding gene: 21243767

Following gene: 77748690

Centisome position: 68.72

GC content: 64.94

Gene sequence:

>1218_bases
ATGAGCTTTCGTGACCCCGCCGACGCCCCCTGTACTGCTGCCACTGCCGCCGTGCGTGCCGGTATCGATCGCGACACCGC
CTACGGTGCGGTGACCCCGCCGATCGTGGTGTCGTCGAATTTCTCGTTCGATGGTTTCGGCAACAAGCGCCAGTACGACT
ACACCCGCAGCGGTAATCCGACCCGCGACCTGTTAGGCGAAGCGCTGGCCGAACTGGAAGGCGGCGCGGGCGGCGTGATC
ACCTCCACCGGCATGGGCGCGATCAATCTGGTGCTCAATGCGGTGCTGCAGCCCGGCGATACGCTGGTGGTGCCGCACGA
TGCGTACGGCGGCAGCTGGCGCCTGTTCAATGCGTTGGCCAAGAAGGGCCACTTCGCGCTGGTCACCGCTGACTTGACCG
ATCCGCGCTCGCTGGCCGATGCATTGGCGCAGTCGCCCAAACTGGTGCTGATCGAAACCCCGTCCAACCCGCTGCTGCGC
ATCACCGATCTGCGCTTCGTCATCGAAGCTGCAAAGAAGGTCGGCGCACTGACGGTGGTGGACAACACCTTCTTGTCGCC
GGCATTGCAGAAGCCGCTGGATTTCGGTGCCGATCTGGTCCTGCATTCCACCACCAAATACATCAACGGCCATAGCGATG
TGGTGGGCGGTGCGGTGGTGGCACGCGATGCCCAGCTGCATCAGCAACTGGTGTGGTGGGCCAATGCGTTGGGCCTGACC
GGCTCGCCGTTCGATGCCTTCCTCACCTTGCGCGGTCTGCGCACGCTGGATGCCCGGCTGCGCGTGCATCAGGAAAACGC
CGATGCGATCGCCGCGCTGCTGGATGGGCATGCGGCGGTGAATCAGGTGTATTTCCCGGGTCTGGCCTCGCATCCGGGCC
ACGCACTGGCCGCGCGCCAGCAGAAGGGCTTCGGGGCGATGATGAGTTTCGAACTGGAAGGCGGCGAAGCGGCCGTGCGT
GCGTTCGTCGATGGCTTGCGTTACTTCACCCTGGCCGAATCGTTGGGCGGCGTGGAAAGCCTGATCGCGCATCCGGCCTC
GATGACGCACGCGGCAATGACCGCCGAAGCGCGTGCGGCAGCCGGCATTTCCGATGGCTTGCTGCGCTTGTCGATCGGGA
TCGAATCGGCCGAAGATCTGCTGGCCGATCTGCGCGCCGGTCTGGCCCGTGCCGAAGCGACCCTGACTAACGCCAGCCGC
AAACAGGTCGACGCGTGA

Upstream 100 bases:

>100_bases
GCGTGTTGCGCTCGCCGTACGGGCACGACGCCTTCCTGAAAGAAATCGATCGCATCGACGCAATCCTCACTACCGCCCTT
CGCAGCACCGGAGAAACCCC

Downstream 100 bases:

>100_bases
GCAATGTCCTGCCCGTTTCGCGCCGCGCTCCGGTCGCTGCAGTGACGCCGACGCCGCGGCTGGCCTTGCTGGGGACCGGC
ACCGTGGGGCGCGCTTTCGT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSFRDPADAPCTAATAAVRAGIDRDTAYGAVTPPIVVSSNFSFDGFGNKRQYDYTRSGNPTRDLLGEALAELEGGAGGVI
TSTGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALAKKGHFALVTADLTDPRSLADALAQSPKLVLIETPSNPLLR
ITDLRFVIEAAKKVGALTVVDNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAQLHQQLVWWANALGLT
GSPFDAFLTLRGLRTLDARLRVHQENADAIAALLDGHAAVNQVYFPGLASHPGHALAARQQKGFGAMMSFELEGGEAAVR
AFVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAAAGISDGLLRLSIGIESAEDLLADLRAGLARAEATLTNASR
KQVDA

Sequences:

>Translated_405_residues
MSFRDPADAPCTAATAAVRAGIDRDTAYGAVTPPIVVSSNFSFDGFGNKRQYDYTRSGNPTRDLLGEALAELEGGAGGVI
TSTGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALAKKGHFALVTADLTDPRSLADALAQSPKLVLIETPSNPLLR
ITDLRFVIEAAKKVGALTVVDNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAQLHQQLVWWANALGLT
GSPFDAFLTLRGLRTLDARLRVHQENADAIAALLDGHAAVNQVYFPGLASHPGHALAARQQKGFGAMMSFELEGGEAAVR
AFVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAAAGISDGLLRLSIGIESAEDLLADLRAGLARAEATLTNASR
KQVDA
>Mature_404_residues
SFRDPADAPCTAATAAVRAGIDRDTAYGAVTPPIVVSSNFSFDGFGNKRQYDYTRSGNPTRDLLGEALAELEGGAGGVIT
STGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALAKKGHFALVTADLTDPRSLADALAQSPKLVLIETPSNPLLRI
TDLRFVIEAAKKVGALTVVDNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAQLHQQLVWWANALGLTG
SPFDAFLTLRGLRTLDARLRVHQENADAIAALLDGHAAVNQVYFPGLASHPGHALAARQQKGFGAMMSFELEGGEAAVRA
FVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAAAGISDGLLRLSIGIESAEDLLADLRAGLARAEATLTNASRK
QVDA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=362, Percent_Identity=40.6077348066298, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI299473759, Length=362, Percent_Identity=38.3977900552486, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI25453487, Length=361, Percent_Identity=36.5650969529086, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1790375, Length=375, Percent_Identity=59.4666666666667, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=33.3333333333333, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17538055, Length=365, Percent_Identity=44.6575342465753, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI17560056, Length=348, Percent_Identity=45.1149425287356, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=35.4054054054054, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319307, Length=387, Percent_Identity=42.6356589147287, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6321254, Length=403, Percent_Identity=32.7543424317618, Blast_Score=196, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6321904, Length=398, Percent_Identity=29.1457286432161, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=27.1889400921659, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6321170, Length=325, Percent_Identity=29.5384615384615, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323557, Length=251, Percent_Identity=27.4900398406374, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322970, Length=281, Percent_Identity=25.6227758007117, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922554, Length=394, Percent_Identity=40.6091370558376, Blast_Score=292, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 42471; Mature: 42339

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRDPADAPCTAATAAVRAGIDRDTAYGAVTPPIVVSSNFSFDGFGNKRQYDYTRSGNP
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
TRDLLGEALAELEGGAGGVITSTGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALA
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
KKGHFALVTADLTDPRSLADALAQSPKLVLIETPSNPLLRITDLRFVIEAAKKVGALTVV
CCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHHHHHCCEEEEE
DNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAQLHQQLVWWANALGLT
CCCHHCHHHHCHHHCCCCEEEECHHHHCCCCCCHHCCHHEEHHHHHHHHHHHHHHHCCCC
GSPFDAFLTLRGLRTLDARLRVHQENADAIAALLDGHAAVNQVYFPGLASHPGHALAARQ
CCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCHHHHHHEECCCCCCCCCCHHHHHH
QKGFGAMMSFELEGGEAAVRAFVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAA
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHH
AGISDGLLRLSIGIESAEDLLADLRAGLARAEATLTNASRKQVDA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
SFRDPADAPCTAATAAVRAGIDRDTAYGAVTPPIVVSSNFSFDGFGNKRQYDYTRSGNP
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
TRDLLGEALAELEGGAGGVITSTGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALA
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
KKGHFALVTADLTDPRSLADALAQSPKLVLIETPSNPLLRITDLRFVIEAAKKVGALTVV
CCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHHHHHCCEEEEE
DNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAQLHQQLVWWANALGLT
CCCHHCHHHHCHHHCCCCEEEECHHHHCCCCCCHHCCHHEEHHHHHHHHHHHHHHHCCCC
GSPFDAFLTLRGLRTLDARLRVHQENADAIAALLDGHAAVNQVYFPGLASHPGHALAARQ
CCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCHHHHHHEECCCCCCCCCCHHHHHH
QKGFGAMMSFELEGGEAAVRAFVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAA
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHH
AGISDGLLRLSIGIESAEDLLADLRAGLARAEATLTNASRKQVDA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]