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The map label for this gene is ptsI [H]

Identifier: 21243706

GI number: 21243706

Start: 3479594

End: 3481324

Strand: Direct

Name: ptsI [H]

Synonym: XAC2979

Alternate gene names: 21243706

Gene position: 3479594-3481324 (Clockwise)

Preceding gene: 21243705

Following gene: 21243707

Centisome position: 67.23

GC content: 67.48

Gene sequence:

>1731_bases
ATGAGCCTGCGCCTGCGCGGACATGGCGCTGCACGCGGCAACGCGCTTGGCCGCGCGCGCGTGCGCCAGAGCCATACCCT
GGAAGTGGCCGAACAACGCGTGCCCGCCAATCAGGTGGAAACGCAGCTGCAGCGCCTGCATGCCGCCATCGCGTCGGCAC
GGGTCGAAATGCAGCAGCTGCGCGACCGCCTGCACGGCGCACTGGCGCGCGAGGTCGGCGAATTTCTCGAGCTGCATGCA
TTGCTGCTTGACGACCCCGAGCTGCTGCAAGGCCTGGATGAGCTGATCCGCACCCACCGTTACAGCGCCGACTATGCCTT
GCGTGTGCAGCGCGACCGCCTGGCGGCGGTGTTCGACGGGATGGAGGATGCGTACCTCAAGAGCCGCATGGACGATCTGG
ATCACGTGATCGGCCGCATCCATGCGTTCCTGCACAAGCGCGCACCCGACCTCAAGGGGGTGGCCGGCGAGATCCTGGTG
TGCGACAACGTGGCACCTTCCGAGCTGGCGCAGCTGCAGGCGCAAGGCGTGGTCGGCATCGTCACCACCGCCGGCAGCAC
GCTGTCGCACAGCGCGATCCTGGCGCGCAGCCTGCACCTGCCGTTGGTGGTAGGCGTCAGCGACGCGCTGCAGCGCATCG
AAGATGGCGATGTGTTGATCGTCGATGCCGGCTCGGGCCAGGTGATCGTCGACCCCAAGCCCGAACATCTGCGCGACTAC
CGTGAGCGCCTGCGCGCGCTGGCCAAGGAACAGCGCGAGCTGGGCCGGCTACGCTCCAAGCCCACGCGCACGCGCGACAA
CGTCGATATCACCCTGCTGGCCAACGCCGAATCGCTGGAAGACGTGGCCAACGCGCATGCGCTCGGGGCCAGCGGCCTGG
GCTTGTATCGCACCGAATTCCTGTTCCTGCAGCGCAACGAGCTGCCGGACGAAGACGAACAGTTCCACACCTACCGCGAC
ACCGTGCTGGGCATGAGCGGCCGCCCGGTCACCATCCGCACCCTGGACCTGGGCGCCGACAAGGCCGACCGCACCGGTCT
GACACTCAGCGATGAGGACAACCCGGCGCTGGGTCTGCGCGGTGTGCGCCTGTCGCTGGCGCGGCCGGCGGTGGCGCAGG
CGCAATTGCGCGCGATCCTGCGGGCGTCCGGCTACGGGCCGGTGCGCATCCTGGTGCCGATGGTCAGCGGCCGCGAAGAA
ATCCTGCAGTTGCGCAAGCAGTTGAAGAAGCTCACCGCGCAGCTGCGCGAGGAAGGCCACGAGATCGCCGAGCACATTCC
GCTGGGCGCGATGATCGAAGTGCCGGCTGCGGCATTGGCGCTGGACTGCTTCATCGACGATATCGATTTCCTGTCCATCG
GCACCAACGACCTGGTGCAGTACCTGCTGGCGGTGGATCGCAACAACGAGGCGTTGGGCGAGCTGTACTCGCCCCTGCAT
CCCGCCGTCCTCCGACTGATCGCGCAGGTCATCGCCACCGGGCGCACCTACGGCAAGCCGGTCGCGGTGTGCGGCGAAAT
CGCCGGCGATCCGCGCTTCGTGCCGATGCTGCTGGCGTTGGGGCTCACCGAGTTCAGCCTGCATCCGGCCACCTTGCTGG
AAGTGCGCCGCGCGGTGCGCGACACCCATCTGGGCGACTTGCGCGTGCACGGCGAAAAATTGCTGCGCGCCCGCGATCGC
AAGGGCATCGAAAAATGGCTGACTGGCCATGCGCAGGGGTTGCTGCGGTAA

Upstream 100 bases:

>100_bases
CTGGTGGACCTGTTCGAGCGTCGTTTCGACGAGGACAGCTGAGCATGCGCGGCGTCGGGGCCGTTGCGACCGACACGGCG
CCGCCGCGCCAGGGACGCGC

Downstream 100 bases:

>100_bases
TTGCGGCACGCACCCGGCCCGGCAGCGCGGCCGATGCTTCAAGACCTGCGTTTCCTTCAAAACGGCGTTCGACACGATTT
CGCAGCCCTGCGGCTGGCCC

Product: phosphotransferase system enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MSLRLRGHGAARGNALGRARVRQSHTLEVAEQRVPANQVETQLQRLHAAIASARVEMQQLRDRLHGALAREVGEFLELHA
LLLDDPELLQGLDELIRTHRYSADYALRVQRDRLAAVFDGMEDAYLKSRMDDLDHVIGRIHAFLHKRAPDLKGVAGEILV
CDNVAPSELAQLQAQGVVGIVTTAGSTLSHSAILARSLHLPLVVGVSDALQRIEDGDVLIVDAGSGQVIVDPKPEHLRDY
RERLRALAKEQRELGRLRSKPTRTRDNVDITLLANAESLEDVANAHALGASGLGLYRTEFLFLQRNELPDEDEQFHTYRD
TVLGMSGRPVTIRTLDLGADKADRTGLTLSDEDNPALGLRGVRLSLARPAVAQAQLRAILRASGYGPVRILVPMVSGREE
ILQLRKQLKKLTAQLREEGHEIAEHIPLGAMIEVPAAALALDCFIDDIDFLSIGTNDLVQYLLAVDRNNEALGELYSPLH
PAVLRLIAQVIATGRTYGKPVAVCGEIAGDPRFVPMLLALGLTEFSLHPATLLEVRRAVRDTHLGDLRVHGEKLLRARDR
KGIEKWLTGHAQGLLR

Sequences:

>Translated_576_residues
MSLRLRGHGAARGNALGRARVRQSHTLEVAEQRVPANQVETQLQRLHAAIASARVEMQQLRDRLHGALAREVGEFLELHA
LLLDDPELLQGLDELIRTHRYSADYALRVQRDRLAAVFDGMEDAYLKSRMDDLDHVIGRIHAFLHKRAPDLKGVAGEILV
CDNVAPSELAQLQAQGVVGIVTTAGSTLSHSAILARSLHLPLVVGVSDALQRIEDGDVLIVDAGSGQVIVDPKPEHLRDY
RERLRALAKEQRELGRLRSKPTRTRDNVDITLLANAESLEDVANAHALGASGLGLYRTEFLFLQRNELPDEDEQFHTYRD
TVLGMSGRPVTIRTLDLGADKADRTGLTLSDEDNPALGLRGVRLSLARPAVAQAQLRAILRASGYGPVRILVPMVSGREE
ILQLRKQLKKLTAQLREEGHEIAEHIPLGAMIEVPAAALALDCFIDDIDFLSIGTNDLVQYLLAVDRNNEALGELYSPLH
PAVLRLIAQVIATGRTYGKPVAVCGEIAGDPRFVPMLLALGLTEFSLHPATLLEVRRAVRDTHLGDLRVHGEKLLRARDR
KGIEKWLTGHAQGLLR
>Mature_575_residues
SLRLRGHGAARGNALGRARVRQSHTLEVAEQRVPANQVETQLQRLHAAIASARVEMQQLRDRLHGALAREVGEFLELHAL
LLDDPELLQGLDELIRTHRYSADYALRVQRDRLAAVFDGMEDAYLKSRMDDLDHVIGRIHAFLHKRAPDLKGVAGEILVC
DNVAPSELAQLQAQGVVGIVTTAGSTLSHSAILARSLHLPLVVGVSDALQRIEDGDVLIVDAGSGQVIVDPKPEHLRDYR
ERLRALAKEQRELGRLRSKPTRTRDNVDITLLANAESLEDVANAHALGASGLGLYRTEFLFLQRNELPDEDEQFHTYRDT
VLGMSGRPVTIRTLDLGADKADRTGLTLSDEDNPALGLRGVRLSLARPAVAQAQLRAILRASGYGPVRILVPMVSGREEI
LQLRKQLKKLTAQLREEGHEIAEHIPLGAMIEVPAAALALDCFIDDIDFLSIGTNDLVQYLLAVDRNNEALGELYSPLHP
AVLRLIAQVIATGRTYGKPVAVCGEIAGDPRFVPMLLALGLTEFSLHPATLLEVRRAVRDTHLGDLRVHGEKLLRARDRK
GIEKWLTGHAQGLLR

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=553, Percent_Identity=36.8896925858951, Blast_Score=355, Evalue=5e-99,
Organism=Escherichia coli, GI1788726, Length=574, Percent_Identity=32.2299651567944, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1789193, Length=541, Percent_Identity=34.0110905730129, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI48994992, Length=414, Percent_Identity=35.2657004830918, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1787994, Length=421, Percent_Identity=25.41567695962, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI226510935, Length=188, Percent_Identity=29.2553191489362, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63413; Mature: 63282

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRLRGHGAARGNALGRARVRQSHTLEVAEQRVPANQVETQLQRLHAAIASARVEMQQL
CCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RDRLHGALAREVGEFLELHALLLDDPELLQGLDELIRTHRYSADYALRVQRDRLAAVFDG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCC
MEDAYLKSRMDDLDHVIGRIHAFLHKRAPDLKGVAGEILVCDNVAPSELAQLQAQGVVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCEEE
VTTAGSTLSHSAILARSLHLPLVVGVSDALQRIEDGDVLIVDAGSGQVIVDPKPEHLRDY
EEECCCHHHHHHHHHHHCCCCEEECHHHHHHHCCCCCEEEEECCCCEEEECCCHHHHHHH
RERLRALAKEQRELGRLRSKPTRTRDNVDITLLANAESLEDVANAHALGASGLGLYRTEF
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCHHHHHE
LFLQRNELPDEDEQFHTYRDTVLGMSGRPVTIRTLDLGADKADRTGLTLSDEDNPALGLR
EEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEECCCCCCCCCCC
GVRLSLARPAVAQAQLRAILRASGYGPVRILVPMVSGREEILQLRKQLKKLTAQLREEGH
CEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
EIAEHIPLGAMIEVPAAALALDCFIDDIDFLSIGTNDLVQYLLAVDRNNEALGELYSPLH
HHHHHCCCCCHHHCCHHHHHHHHHHCCCHHEECCHHHHHHHHHHHCCCCHHHHHHHCCCC
PAVLRLIAQVIATGRTYGKPVAVCGEIAGDPRFVPMLLALGLTEFSLHPATLLEVRRAVR
HHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHH
DTHLGDLRVHGEKLLRARDRKGIEKWLTGHAQGLLR
HCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLRLRGHGAARGNALGRARVRQSHTLEVAEQRVPANQVETQLQRLHAAIASARVEMQQL
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RDRLHGALAREVGEFLELHALLLDDPELLQGLDELIRTHRYSADYALRVQRDRLAAVFDG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCC
MEDAYLKSRMDDLDHVIGRIHAFLHKRAPDLKGVAGEILVCDNVAPSELAQLQAQGVVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCEEE
VTTAGSTLSHSAILARSLHLPLVVGVSDALQRIEDGDVLIVDAGSGQVIVDPKPEHLRDY
EEECCCHHHHHHHHHHHCCCCEEECHHHHHHHCCCCCEEEEECCCCEEEECCCHHHHHHH
RERLRALAKEQRELGRLRSKPTRTRDNVDITLLANAESLEDVANAHALGASGLGLYRTEF
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCHHHHHE
LFLQRNELPDEDEQFHTYRDTVLGMSGRPVTIRTLDLGADKADRTGLTLSDEDNPALGLR
EEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEECCCCCCCCCCC
GVRLSLARPAVAQAQLRAILRASGYGPVRILVPMVSGREEILQLRKQLKKLTAQLREEGH
CEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
EIAEHIPLGAMIEVPAAALALDCFIDDIDFLSIGTNDLVQYLLAVDRNNEALGELYSPLH
HHHHHCCCCCHHHCCHHHHHHHHHHCCCHHEECCHHHHHHHHHHHCCCCHHHHHHHCCCC
PAVLRLIAQVIATGRTYGKPVAVCGEIAGDPRFVPMLLALGLTEFSLHPATLLEVRRAVR
HHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHH
DTHLGDLRVHGEKLLRARDRKGIEKWLTGHAQGLLR
HCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]